Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function

A capillary electrophoresis, microfluidic chip technology, applied in enzymology/microbiology devices, biochemical cleaning devices, bioreactors/fermenters for specific purposes, etc., can solve the cost of multiple DNA detection reagents and instruments, unfavorable instruments Miniaturization and portability, consumption and other issues, to achieve the effect of facilitating miniaturization and portability, easy integration, and reducing the cost of reagents and instruments

Inactive Publication Date: 2013-01-30
张影频
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  • Application Information

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Problems solved by technology

[0023] The purpose of the present invention is to provide a microfluidic chip device integrating continuous flow PCR and capillary electrophoresis functions, which solves the problem of continuous flow PCR amplifi

Method used

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  • Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function
  • Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function
  • Micro fluidic chip apparatus by integrating continuous flow PCR and capillary electrophoresis function

Examples

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Embodiment 1

[0051]Embodiment 1 CF-PCR-CE functional integrated chip and its preparation

[0052] 1. CF-PCR-CE chip structure

[0053] Utilizing the good optical properties of glass and the low-cost characteristics of PDMS to facilitate the formation of micro-channels, a micro-flow chip with a glass-PDMS structure is fabricated. Because temperature is required for performing PCR, either the heater and the temperature sensor are integrated on the chip, or the heater and the temperature sensor are separated from the chip. The structure separating the heater and temperature sensor from the chip is shown in the figure. Using soft lithography technology, the number of micro flow circulation and separation channels with optional amplification number can be fabricated on the upper PDMS cover sheet, and the lead-in and lead-out through holes are made by drilling technology, and the bonding technology is used to bond with the lower glass substrate. Together they make a microfluidic channel chip. ...

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Abstract

The invention discloses a micro fluidic chip apparatus by integrating a continuous flow PCR and a capillary electrophoresis function, which comprises a continuous flow type PCR chip and a capillary electrophoresis chip, and is characterized in that an output channel of the continuous flow type PCR chip is directly connected in a sample pool of the capillary electrophoresis chip, a sample introduction channel and a separating channel are provided on the capillary electrophoresis chip, the sample introduction channel and the separating channel present an intersect shape, the top of the sample introduction channel is the sample pool and a sample waste liquid pool, and the top of the separating channel is a buffer liquid pool and a waste liquid pool. The apparatus provided by the invention can enhance the automation degree and whole process operation speed for DNA detection, the operation step is reduced, and the apparatus is convenient for miniaturization and portable performance of the apparatus.

Description

technical field [0001] The invention belongs to the technical field of molecular biological detection, and in particular relates to a microfluidic chip device integrating the functions of continuous flow PCR and capillary electrophoresis. Background technique [0002] Polymerase chain reaction (PCR), also known as cell-free molecular cloning or specific DNA sequence in vitro primer-directed enzymatic amplification technology, is a rapid in vitro DNA amplification technology, as a main means of amplification and replication, It has been applied in a wide range of fields such as medical diagnosis, gene analysis, and forensic identification, and has gradually become one of the core technologies for nucleic acid analysis and amplification, and has had a profound impact on gene-related research. [0003] PCR technology manipulates the replication of DNA molecules by regulating the activity of polymerase at certain sites in the DNA. This reaction has the outstanding advantages of ...

Claims

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Application Information

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IPC IPC(8): C12M1/00C12M1/34
Inventor 杨楠艾洪新何越臧伯玮
Owner 张影频
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