PCR chip micro-system and method for preparing the same
A chain reaction and polymerase technology, applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactor/fermenter combination, etc., can solve problems such as large systems and complex operations
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[0032] The PCR chip microsystem of the present invention includes a polymerase chain reaction chip and a thermal cycle system for DNA amplification. , The liquid outlet is connected with each reaction pool through the flow channel. A reaction pool with a volume of several thousand nanoliters can be designed on a chip of a few square centimeters, and different PCR reactions can be carried out in the reaction pool by using the theory of oligonucleotide polymorphism of biomolecules. The number of the above-mentioned reaction pools is determined according to the needs. The volume of the reaction pool is between nanoliters (nL) and milliliters (mL). The reaction pools can be in the shape of square, rectangle, circle, trapezoid, etc. Semicircle, trapezoid and other shapes.
[0033]The thermal cycle system includes a micro-heater, a micro-sensor and a temperature control system. The micro-heater and the micro-sensor can be resistive materials such as Pt, Al, and Au, and are prepared...
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