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PCR chip micro-system and method for preparing the same

A chain reaction and polymerase technology, applied in biochemical equipment and methods, enzymology/microbiology devices, bioreactor/fermenter combination, etc., can solve problems such as large systems and complex operations

Inactive Publication Date: 2006-07-19
PEKING UNIV
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Problems solved by technology

Traditional PCR is completed on a 96-well plate with a reaction chamber volume of tens to hundreds of microliters. After PCR, the DNA sample needs to go through electrophoresis, fluorescence detection, data analysis and other processes, which takes about several hours, and the entire The system is huge and the operation is complicated

Method used

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  • PCR chip micro-system and method for preparing the same
  • PCR chip micro-system and method for preparing the same
  • PCR chip micro-system and method for preparing the same

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Embodiment Construction

[0032] The PCR chip microsystem of the present invention includes a polymerase chain reaction chip and a thermal cycle system for DNA amplification. , The liquid outlet is connected with each reaction pool through the flow channel. A reaction pool with a volume of several thousand nanoliters can be designed on a chip of a few square centimeters, and different PCR reactions can be carried out in the reaction pool by using the theory of oligonucleotide polymorphism of biomolecules. The number of the above-mentioned reaction pools is determined according to the needs. The volume of the reaction pool is between nanoliters (nL) and milliliters (mL). The reaction pools can be in the shape of square, rectangle, circle, trapezoid, etc. Semicircle, trapezoid and other shapes.

[0033]The thermal cycle system includes a micro-heater, a micro-sensor and a temperature control system. The micro-heater and the micro-sensor can be resistive materials such as Pt, Al, and Au, and are prepared...

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Abstract

The portable micro PCR chip system prepared by MEMS or micro-model technique in bio-micro EMC system field comprises some trap-type reaction ponds for DNA amplification connected to liquid inlet / outlet by flow passages, an integrated micro-heater and sensor for amplifying DNA by the temperature cycle control system, a micro-pump and micro-valve on pond inlet to lead the DNA initiator and ponds insulation, and maybe a fluorescence excitation source, a detector and a fluorescence signal detection and analysis system for on-line detection on PCR product. This chip system provides a high-flux and fast DNA amplification means with low cost and well reliability.

Description

technical field [0001] The invention relates to the technical field of micro-biological chip systems, in particular to the design and manufacturing method of a polymerase chain reaction chip microsystem. Background technique [0002] Polymerase chain reaction (PCR) technology is a common technique for amplifying DNA, and it is also an indispensable means for micro / trace level detection of biomolecules. PCR is a three-temperature cycle process of denaturation, annealing, and extension at a specific temperature. Every time a temperature cycle is completed, the number of DNA molecules doubles, that is, the DNA doubles in 2 n The format increases, and after 20-30 temperature cycles, the DNA will be amplified millions of times. Traditional PCR is completed on a 96-well plate with a reaction chamber volume of tens to hundreds of microliters. The DNA sample after PCR needs to go through electrophoresis, fluorescence detection, data analysis and other processes, which takes about s...

Claims

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Application Information

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IPC IPC(8): C12P19/34C12M1/00C12Q1/68
Inventor 于晓梅李修函张大成李婷
Owner PEKING UNIV
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