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Nucleic acid exparding gene chip hybridization intellectualization and inspecting instrument thereof

A technique for amplifying genes and chip hybridization, which is applied in biochemical equipment and methods, and microbial measurement/inspection. Positive results, effects of shortening PCR time

Inactive Publication Date: 2003-04-02
赵雨杰
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Problems solved by technology

However, due to defects such as false positives and complicated operation in PCR, there are many disadvantages in clinical application, because: 1. Instability factors of many test conditions in the PCR process lead to false amplification of PCR
2. The judgment of PCR products is to use electrophoresis to judge whether a nucleic acid fragment of a specific length is obtained, and its specific sequence cannot be determined
3. The PCR process is cumbersome and easily polluted

Method used

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  • Nucleic acid exparding gene chip hybridization intellectualization and inspecting instrument thereof
  • Nucleic acid exparding gene chip hybridization intellectualization and inspecting instrument thereof
  • Nucleic acid exparding gene chip hybridization intellectualization and inspecting instrument thereof

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Embodiment Construction

[0032] As shown in the figure, the nucleic acid amplification gene chip hybridization intelligent detection method is characterized by the following steps:

[0033] (A) Send the PCR chip into the temperature control area, and complete the denaturation, annealing, and extension processes of the PCR process according to the set temperature mode;

[0034] (B) After the PCR cycle process is completed, the PCR chip is moved to the hybridization area, and the temperature of the entire hybridization process is controlled according to the set temperature;

[0035] (C) After the hybridization process is completed, the PCR chip is cleaned by the cleaning device. After the cleaning is completed, the gene chip is sent into the detection area by the transmission device for detection. The position signal of the detection device and the position signal of the hybridization point of the PCR chip are determined by the sensor, The photoelectric converter is output to the computer, the analog si...

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Abstract

An intelligent parallel detection instrument for the hybridization of PCR chips is composed of temp cycling unit, hybridizing unit, 3D transfer manipulator, detecting unit and computer. Said detecting unit has position sensor and photoelectric converter. Its detection process includes such steps as introducing PCR chip to temp controlled region, performing PCR procedure, moving PCR chip to hybridizing region, performing hybridizing procedure, washing, testing and processing the tested result.

Description

technical field [0001] The invention belongs to the field of biochip detection, and in particular relates to a nucleic acid amplification gene chip hybridization intelligent parallel detection method and a detection device thereof. Background technique [0002] The Human Genome (Sequencing) Project (Humen genome project HGP) is one of the greatest feats in the history of human natural science. With the gradual implementation of the Human Genome Project and the rapid development of molecular biology-related disciplines, HGP has completed the production of genetic maps ahead of schedule, and has determined 800,000 cDNA fragments (ESTs), equivalent to 450,000 genes, accounting for the total human genes. about 50%. Complete sequence analysis is expected to be completed in 2003. More and more animal, plant, and microbial genome sequences have been determined, and genetic sequence data is growing rapidly at an unprecedented rate. However, how to study the func...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 赵雨杰段晓军何钦成肖玉平
Owner 赵雨杰
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