Nucleic acid expanding gene chip hybridization intellectualization and inspecting instrument thereof

Abstract

The invention belongs to the field of biochip detection, and particularly relates to a nucleic acid amplification gene chip hybridization intelligent parallel detection method and a detection device thereof, which are characterized in that: a temperature cycler (3), a hybridization instrument (4), a three-dimensional transfer manipulator (5), detection mechanism (7), computer (8); Position sensor and photoelectric converter are provided with in described detection mechanism; Described position sensor and photoelectric converter are electrically connected with computer (8); When detecting, first Send the PCR chip into the temperature control area; after the PCR cycle process is completed, move the PCR chip to the hybridization area; after the hybridization process is completed, clean it, and then perform detection after cleaning; after the computer processes the detection data, it gives process result.

Description

technical field [0001] The invention belongs to the field of biochip detection, and in particular relates to a nucleic acid amplification gene chip hybridization intelligent parallel detection method and a detection device thereof. Background technique [0002] The Human Genome (Sequencing) Project (Humen genome project HGP) is one of the greatest feats in the history of human natural science. With the gradual implementation of the Human Genome Project and the rapid development of molecular biology-related disciplines, HGP has completed the production of genetic maps ahead of schedule, and has determined 800,000 cDNA fragments (ESTs), equivalent to 450,000 genes, accounting for the total human genes. about 50%. Complete sequence analysis is expected to be completed in 2003. More and more animal, plant, and microbial genome sequences have been determined, and genetic sequence data is growing rapidly at an unprecedented rate. However, how to study the ...

AI Technical Summary

Problems solved by technology

However, due to the defects of false positives and complicated operation in PCR, there are many disadvantages in clinical application.

The reason is due to: 1. The unstable factors of many experimental conditions in the PCR process lead to the false amplification of PCR

2. The judgment of PCR products is to use electrophoresis to judge whether a nucleic acid fragment of a specific length is obtained, and its specific sequence cannot be determined

3. The PCR process is cumbersome and easily polluted

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