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A method and system for rapid y-str typing of male individuals

A Y-STR and individual technology, applied in the field of paternity testing, can solve problems such as the increase of time shadow bands, the difficulty of interpreting DNA typing results, and the loss of alleles

Active Publication Date: 2019-11-26
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when rapid PCR shortens the time, it will also bring some problems such as increased shadow bands, non-specific amplification, disruption of the balance between loci, or loss of alleles, etc., which will cause serious problems to the interpretation of DNA typing results. difficulty

Method used

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  • A method and system for rapid y-str typing of male individuals
  • A method and system for rapid y-str typing of male individuals
  • A method and system for rapid y-str typing of male individuals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1, verification of the accuracy of the method and system for rapidly obtaining male individual Y chromosome STR typing of the present invention

[0052] In this embodiment, 100 male individual blood samples are used. These samples are samples collected by the applicant from the Physical Evidence Identification Center of the Ministry of Public Security. The source of the individual is unknown, and the method and system of this application are used to type the Y chromosome STR, including:

[0053] 1) Utilize the DNA extraction system in the system of the present invention to extract the DNA of male individuals, 2) Utilize the composite detection system in the system of the present invention to obtain the typing results of the 16 Y chromosome STR loci of the DNA, the STR gene The loci are DYS458, DYS390, DYS438, DYS392, DYS393, DYS437, DYS385a / b, GATA_H4, DYS391, DYS447, DYS19, DYS448, DYS456, DYS635 and DYS439. 3) According to the genotypes of the 16 STR loci, ...

Embodiment 2

[0075] Embodiment 2 The present invention compares the system of male individual Y chromosome STR typing with the existing Y chromosome STR typing kit

[0076] 100 parts of DNA samples of Example 1 are carried out typing of 16 loci with the system of the present application, adopt existing Y chromosome STR typing kit DNATyper Y21 (being conventional detection system) to carry out the typing of above-mentioned sample Y chromosome locus simultaneously Typing, such as Figure 6 (the typing map of 16 loci obtained by the method and system of the present invention) and Figure 7 (The typing map of 16 loci obtained by using the DNATyperY21 kit) is shown.

[0077] With the system of the present invention, compared with the existing Y chromosome STR typing kit (compared, the DNA detection results obtained on the same locus are consistent, and the detection rate all reaches 100%. The results are shown in Table 3, and each group of experiments repeats the results Comprehensive analysi...

Embodiment 3

[0080] Embodiment 3 The sensitivity of the method and system result of the present invention

[0081] Get DNA standard product 9948 10ng, 5ng, 2.5ng, 1.25ng, 625pg, 313pg, 157pg, 78.5pg, 39.3pg and 19.7pg respectively, amplify and detect according to the method provided by the present invention, repeat 3 times in parallel, the result is as follows Figure 3-Figure 4 shown, where image 3 Shows the typing pattern of 16 loci of the standard DNA 9948 (10ng) obtained by the method and system of the present invention, Figure 4 The statistical results of the sensitivity test of the system of the present invention are shown.

[0082] Depend on Figure 4 It can be seen that the optimum DNA template amount of the inventive method is between 157pg~1.25ng, and when the template amount is 78.5pg or below, although complete STR typing can still be obtained, some allele peak heights are lower than 50RFU; When the amount of DNA template is 39.3pg or lower, alleles may be lost, and comple...

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Abstract

The invention provides method and system for Y-STR typing of an individual man. The method comprises the following steps: extracting the DNAs of the individual man to acquire the genotypes of 16 Y-chromosome STR gene loci of the DNAs, wherein the STR gene loci are DYS458, DYS390, DYS438, DYS392, DYS393, DYS437, DYS385a / b, GATA_H4, DYS391, DYS447, DYS19, DYS448, DYS456, DYS393, DYS635 and DYS439; and acquiring the Y-STR typing results of the individual man according to the 16 STR gene loci of the individual man. The invention also provides a system for rapid Y-STR typing of the individual man. The method and system provided by the invention can realize rapid, stable and high-quality Y-chromosome STR typing and apply the Y-STR typing results to rapid identification of patriarchal relatives, family checking and the like.

Description

technical field [0001] The present invention relates to a method and system for paternity identification, in particular to a method and system for rapid Y-STR typing of male individuals. Background technique [0002] Since Jeffreys in the United Kingdom first applied DNA fingerprinting to the paternity test of an immigration case in 1985, after nearly 30 years of development, DNA typing technology is not only used in biological evidence testing in routine cases, but also in some difficult and Handling of urgent cases, such as finding evidence during statutory detention or surveillance times or life-threatening international security issues. However, conventional detection methods require DNA extraction and PCR amplification before DNA analysis can be performed. This process takes 8-9 hours, of which PCR amplification takes 3-4 hours, which is the most time-consuming step in the inspection process. , In order to shorten the reaction time and improve the inspection efficiency...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858
CPCC12Q1/6858C12Q1/6888C12Q2600/156C12Q2600/16C12Q2531/113C12Q2537/143C12Q2525/151
Inventor 孙敬欧元李彩霞韩俊萍孙辉李万水叶健
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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