Method and system for individual recognition and paternity identification of unknown sample

A technology of paternity identification and material inspection, which is applied in the direction of biochemical equipment and methods, microbiological determination/inspection, etc. It can solve the problems of being unable to meet the requirements of forensic DNA rapid inspection and on-site inspection capabilities, shortening the detection time, etc.

Active Publication Date: 2014-11-05
INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing STR typing technology is limited by various factors (including the state of the sample material, primers, amplification sites, amplification conditions, and the amplification system used, etc.), and the PCR process alone takes more than 3 months. In the past few hours, scholars have conducted research on shortening the PCR amplification time by fast DNA polymerase, fast thermal cycler, microfluidic chip technology, etc., but none of them have achieved the ideal effect of shortening the detection time.
With the increase of emergencies, the existing detection system cannot meet the requirements of public security for the rapid detection and on-site detection of forensic DNA in actual combat

Method used

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  • Method and system for individual recognition and paternity identification of unknown sample
  • Method and system for individual recognition and paternity identification of unknown sample
  • Method and system for individual recognition and paternity identification of unknown sample

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Example 1. Verification of the accuracy of the method and system for individual identification and paternity identification of unknown samples of the present invention

[0059] In this example, the unknown samples are 120 fresh human anticoagulants (60 males, 60 females), these samples are samples collected by the applicant from the Zhongguancun Community Hospital in Beijing, and their individual sources are known , but in the implementation process of Example 1 of the present application, it is assumed that its individual origin is unknown, and the method and system of the present application are used to carry out individual identification and parental identification, including:

[0060] 1) Utilize the DNA extraction system in the system of the present invention to extract the DNA of the unknown test material, 2) Use the composite detection system in the system of the present invention to obtain the DNA including 14 autosomal STR loci and 2 Y chromosome loci , and the ...

Embodiment 2

[0082] Example 2 Comparison between the system of the present invention for individual identification and paternity identification of unknown samples and the existing kit for individual identification

[0083] The 120 DNA samples of Example 1 were used for the typing of 17 loci (the typing process was the same as Example 1, except that the number of cycles in the amplification process was 28), and the existing DNATyper15 TMThe kit (that is, the conventional detection system) performs typing on the autosomal locus of the above sample (using the parameters of the kit itself), and the typing result is used as a positive control for the autosomal locus, Yfiler TM The kit performs typing on the Y chromosome locus (using the parameters of the kit itself), the typing result is used as a positive control for the Y chromosome locus, and a blank reagent is set as a negative control, which is repeated 3 times in parallel.

[0084] DNA typing map and DNATyper15 obtained by the system of ...

Embodiment 3

[0089] Example 3 Sensitivity of the results of the method and system of the present invention

[0090] Take 2ng, 1ng, 0.75ng, 0.5ng, 0.25ng, and 0.125ng of standard DNA samples respectively, carry out amplification and detection according to the method provided by the present invention, and repeat three times in parallel, the results are as follows Figure 5 shown.

[0091] The results are shown in Table 4. It can be seen that the optimal DNA template amount of the method of the present invention is between 0.5 and 2 ng. For the sample material with a DNA concentration greater than 0.5 ng / μL, the detection rate can reach 100%, and the detection line is slightly lower. At the current detection level of STR detection kits (0.125ng) commonly used in the field of forensics, the amplification time of about 1 hour is significantly shorter than these conventional kits, which usually require an amplification time of about 3 hours.

[0092] Table 4 DNA detection results for sensitivit...

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Abstract

The invention provides a method and system for individual recognition and paternity identification of an unknown sample. The method includes: extracting the DNA of the unknown sample; acquiring the typing result of 17 loci contained by the DNA, i.e. 14 autosome STR loci, 2 Y-chromosome loci, and the sex determination locus Amelogenin, with the STR loci being D6S1043, D21S11, D7S820, CSF1PO, D2S1338, D3S1358, D13S317, D8S1179, D16S539, Penta E, D5S818, vWA, D18S51 and FGA, the Y-STR locus being DYS448, and the Y-chromosome insertion/deletion site being M134; and carrying out individual recognition and paternity identification according to the genotypes of the 17 loci of the unknown sample. The invention also provides the system for individual recognition and paternity identification of the unknown sample. The scheme involved in the invention can realize simultaneous detection of autosome STR loci and Y-chromosome loci, and also can shorten the detection time, thus improving the efficiency of individual recognition.

Description

technical field [0001] The present invention relates to a method and system for individual identification and paternity identification, in particular to a method and system for individual identification and paternity identification for unknown test materials. Background technique [0002] STR typing technology has become the core of the second generation of forensic DNA typing technology since the 1990s due to its advantages of good specificity, high detection sensitivity, and multi-site amplification. However, the existing STR typing technology is limited by various factors (including the state of the test material, primers, amplification sites, amplification conditions, amplification system used, etc.), and the PCR process alone takes more than three times. In the past few hours, some scholars have conducted researches on shortening PCR amplification time, such as rapid DNA polymerase, rapid thermal cycler, and microfluidic chip technology, but none of them have achieved t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/156
Inventor 李彩霞孙敬韩俊萍赵兴春刘鹏
Owner INST OF FORENSIC SCI OF MIN OF PUBLIC SECURITY
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