Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform

A technology of 3D printing and manufacturing method, applied in the field of PCR biochip research, can solve the problems of unstable amplification results, large consumption of reagents, cumbersome operation, etc., and achieve precise control of droplet volume, large dynamic range, and cost reduction. Effect

Active Publication Date: 2013-04-03
INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
View PDF4 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although conventional PCR technology has been widely used in life science research and related fields, and has been continuously improved during the applic

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform
  • Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform
  • Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0033] The manufacturing method of the ink-jet digital PCR chip of the present invention is as follows: first prepare a clean substrate; then use the 3D printing platform to control the nozzle to quantitatively perform array printing of the PCR reaction reagent and sealing oil on the surface of the substrate respectively , forming an arrayed liquid-in-oil droplet structure; finally sealed and fixed to form a packaged digital PCR reaction chip.

[0034] see figure 1 , figure 2 with image 3 As shown, the present invention provides a method for manufacturing a digital PCR chip based on a substrate with a microwell array and a 3D printing platform (first embodiment). Specific steps are as follows:

[0035] Step 1: Take a substrate 10 with a microhole array structure 11, the material of the substrate 10 is any non-biologically toxic material such as quartz glass, silicon wafer, silicon dioxide wafer, optical fiber panel or organic sheet material. The specific preparation meth...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for manufacturing a digital PCR (polymerase chain reaction) chip based on a 3D (three-dimensional) printing platform. The method comprises the steps as follows: step one, a substrate with a micropore array structure is adopted; step two, the substrate is subjected to surface treatment; step three, the substrate is placed on the 3D printing platform; step four, by means of a plurality of nozzles on the 3D printing platform, the substrate is subjected to quantitive jet printing in oil drop and liquid drop manners alternately, so that a liquid drop in oil array structure is formed in a micropore on the surface of the substrate; step five, the micropore array structure on the substrate is filled with excess oil; step six, a transparent cover sheet is adopted; step seven, the transparent cover sheet is fixed on the substrate for covering, so as to finish the preparation. According to the method for manufacturing the PCR chip based on the 3D printing platform, a 3D printing technology is adopted to build a picoliter magnitude reaction system and achieve an amplified reaction in the system, therefore, the efficiency is high, the cost is low, and the maximum possibility is provided for the digital PCR productization.

Description

technical field [0001] The invention relates to the field of PCR biochip research, in particular to a method for manufacturing a digital PCR chip based on a 3D printing platform. Background technique [0002] When carrying out biological research and actual detection, sometimes the amount of the DNA sample to be tested is small or the concentration of the DNA molecule to be tested extracted from the sample is low, in order to obtain better results, it is usually necessary to aggregate the amount of the sample molecule first Enzyme chain reaction (Polymerase Chain Reaction, PCR). Although conventional PCR technology has been widely used in life science research and related fields, and has been continuously improved during the application process, there are still shortcomings such as time-consuming, cumbersome operation, large consumption of reagents, and unstable amplification results. . Therefore, people have been looking for a faster and easier PCR method. [0003] Digit...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/00
Inventor 孙英男魏清泉俞育德周晓光韩伟静
Owner INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap