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Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform

A technology of 3D printing and manufacturing method, applied in the field of PCR biochip research, can solve the problems of unstable amplification results, large consumption of reagents, cumbersome operation, etc., and achieve precise control of droplet volume, large dynamic range, and cost reduction. Effect

Active Publication Date: 2013-04-03
INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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Problems solved by technology

Although conventional PCR technology has been widely used in life science research and related fields, and has been continuously improved during the application process, there are still shortcomings such as time-consuming, cumbersome operation, large consumption of reagents, and unstable amplification results.

Method used

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  • Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform
  • Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform
  • Method for manufacturing digital PCR (polymerase chain reaction) chip based on 3D (three-dimensional) printing platform

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Embodiment Construction

[0033] The manufacturing method of the ink-jet digital PCR chip of the present invention is as follows: first prepare a clean substrate; then use the 3D printing platform to control the nozzle to quantitatively perform array printing of the PCR reaction reagent and sealing oil on the surface of the substrate respectively , forming an arrayed liquid-in-oil droplet structure; finally sealed and fixed to form a packaged digital PCR reaction chip.

[0034] see figure 1 , figure 2 with image 3 As shown, the present invention provides a method for manufacturing a digital PCR chip based on a substrate with a microwell array and a 3D printing platform (first embodiment). Specific steps are as follows:

[0035] Step 1: Take a substrate 10 with a microhole array structure 11, the material of the substrate 10 is any non-biologically toxic material such as quartz glass, silicon wafer, silicon dioxide wafer, optical fiber panel or organic sheet material. The specific preparation meth...

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Abstract

The invention discloses a method for manufacturing a digital PCR (polymerase chain reaction) chip based on a 3D (three-dimensional) printing platform. The method comprises the steps as follows: step one, a substrate with a micropore array structure is adopted; step two, the substrate is subjected to surface treatment; step three, the substrate is placed on the 3D printing platform; step four, by means of a plurality of nozzles on the 3D printing platform, the substrate is subjected to quantitive jet printing in oil drop and liquid drop manners alternately, so that a liquid drop in oil array structure is formed in a micropore on the surface of the substrate; step five, the micropore array structure on the substrate is filled with excess oil; step six, a transparent cover sheet is adopted; step seven, the transparent cover sheet is fixed on the substrate for covering, so as to finish the preparation. According to the method for manufacturing the PCR chip based on the 3D printing platform, a 3D printing technology is adopted to build a picoliter magnitude reaction system and achieve an amplified reaction in the system, therefore, the efficiency is high, the cost is low, and the maximum possibility is provided for the digital PCR productization.

Description

technical field [0001] The invention relates to the field of PCR biochip research, in particular to a method for manufacturing a digital PCR chip based on a 3D printing platform. Background technique [0002] When carrying out biological research and actual detection, sometimes the amount of the DNA sample to be tested is small or the concentration of the DNA molecule to be tested extracted from the sample is low, in order to obtain better results, it is usually necessary to aggregate the amount of the sample molecule first Enzyme chain reaction (Polymerase Chain Reaction, PCR). Although conventional PCR technology has been widely used in life science research and related fields, and has been continuously improved during the application process, there are still shortcomings such as time-consuming, cumbersome operation, large consumption of reagents, and unstable amplification results. . Therefore, people have been looking for a faster and easier PCR method. [0003] Digit...

Claims

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Application Information

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IPC IPC(8): C12M1/00
Inventor 孙英男魏清泉俞育德周晓光韩伟静
Owner INST OF SEMICONDUCTORS - CHINESE ACAD OF SCI
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