Microfluidic PCR (Polymerase Chain Reaction) chip fluorescence fluid detection device based on CCD (Charge Coupled Device) image sensor

Abstract

The invention relates to a microfluidic PCR (Polymerase Chain Reaction) chip fluorescence fluid detection device based on a CCD (Charge Coupled Device) image sensor. The device is composed of a spatial three-dimensional micro-channel, a CCD image sensor, a monochromatic LED (Light Emitting Diode) light source, a microprocessor and a liquid crystal display device, and the device is mainly used for carrying out fluorescence detection of a PCR amplification reaction in a spatial range and integrating signal generation, signal collection and transmission and signal processing and recognition parts in signal detection to achieve integration, automatic detection and control of a miniature microfluidic real-time fluorescence PCR detection system, thereby avoiding optical path transmission of an optical path composed of various optical devices and optical fibers, simplifying the detection structure and facilitating the integration of the system.

Description

technical field [0001] The invention relates to a fluorescent signal detection device for microfluidic reagents in biological PCR chips, which belongs to the technical field of CCD image sensors, and in particular to a fluorescent fluid detection device for microfluidic PCR chips based on CCD image sensors. Background technique [0002] Polymerase chain reaction (PCR) is a nucleic acid amplification technology that simulates the natural DNA replication process in vitro, that is, asexual cell molecular cloning technology. Its principle is similar to the replication of natural DNA. A method of sexual DNA. The PCR microfluidic biochip integrates the functions of sampling, dilution, reagent addition, reaction, separation and detection into one chip. Its scientific and advanced nature is reflected in the two aspects of structural miniaturization and functional integration. There are four basic points in biochip technology: chip preparation, sample preparation, biochemical reacti...

AI Technical Summary

Problems solved by technology

Due to the large volume of the component itself and the use of separate components, a supporting optical path device is required, resulting in a large volume of the entire fluorescence detection device;

[0005] 2. Since the optical path composed of various optical devices and optical fibers is required for the transmission of excitation light and the collection of reflected light, not only the structure is complex and it is difficult to achieve integ

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