Glass capillary minitype polymerase chain reaction (PCR) system and preparation method thereof

Abstract

The invention discloses a glass capillary minitype polymerase chain reaction (PCR) system and a preparation method thereof. The PCR system comprises a chip base, a PCR chip and a fan. The PCR chip is composed of glass capillary and a bottom plate. The glass capillary is internally provided with a PCR reaction chamber. A heating electrode is arranged on the lower surface of the bottom plate, a temperature measuring electrode and a deep groove are formed in the upper surface of the bottom plate, the glass capillary is embedded into the deep groove, and the deep groove is filled with heat conduction resin. The PCR chip reaction chamber is the glass capillary and can be used for achieving the PCR chip which is quick in temperature rising and reducing and temperature uniformity and high in amplification efficiency. The consumable item in the glass capillary minitype PCR chip is the glass capillary, the bottom plate with the heating electrode, the temperature measuring electrode and the deep groove can be used for multiple times, cross contamination is avoided, and the use cost of the chip is greatly reduced.

Description

Technical field [0001] The invention relates to a polymerase chain reaction (PCR) system, in particular to a glass capillary micro PCR system and a preparation method thereof. Background technique [0002] Polymerase chain reaction (PCR) is a molecular biology technique used to amplify and amplify specific DNA fragments. It is a special DNA replication in vitro that can greatly increase trace amounts of DNA. The idea was first proposed by the American scientist K.B. Mullis in 1983, who invented the polymerase chain reaction in 1985, which meant the real birth of PCR technology. After more than 30 years of development, PCR technology has been widely used in fields such as health analysis and disease diagnosis, agriculture, ecological environment monitoring, and criminal case analysis. [0003] The PCR chip based on MEMS microfluidic technology miniaturizes the traditional PCR reaction device, thereby greatly reducing the volume, power consumption, and price of the device, reducing ...

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Problems solved by technology

[0005] However, the integrated microfluidic chip disclosed in the above patent is a non-reusable device, resulting in relatively high cost

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