Micro-droplet type PCR (polymerase chain reaction) chip and manufacture method thereof

Abstract

The invention provides a micro-droplet type PCR (polymerase chain reaction) chip and a manufacture method thereof. The upper layer of the chip is a PDMS (polydimethylsiloxane) imaging cover plate formed by connecting a DNA (deoxyribonucleic acid) target fragment with a primer injection port, a single phase drop forming microstructure, a PCR reaction enzyme and an mRNA (messenger ribonucleic acid) substrate injection port, an oil phase reagent injection port, a drop straight line channel, a cross hybrid channel, a micro drop buffer channel, a micro drop PCR reaction chamber and a micro drop PCR waste liquid chamber, the lower layer of the chip is a glass substrate, and the upper layer and the lower layer are bonded and packaged to form a complete micro drop PCR chip. The manufacture method of the chip comprises the steps of firstly, manufacturing a mask plate; secondly, molding; thirdly, pouring a die; and fourthly, taking a figure. The PDMS figure at the upper layer of the chip adopts a multilayer filter structure, and the formed micro drop has a uniform size, stable grain diameter and strong controllability. Compared with traditional PCR chips, the micro drop PCR chip manufactured by the method has great advantages in hybrid effect, reaction efficiency, and high throughput.

Description

technical field [0001] The invention relates to the field of biomedical application instruments, in particular to a micro-droplet PCR chip and a manufacturing method thereof. Background technique [0002] Polymerase chain reaction (Polymerase Chain Reaction, PCR), or cell-free cloning technique (Free bacteria cloning technique), as an important technology in molecular biology and genetic engineering, it is a selective amplification method under the guidance of primers. The method for increasing DNA and RNA fragments has outstanding advantages such as specificity, sensitivity, high yield, rapidity, simplicity, good repeatability, and easy automation. The PCR amplification instrument used in bioengineering at this stage can continuously amplify the required target gene fragments multiple times, and can replicate to a number of millions of times. It is widely used in the purpose of obtaining target genes or gene fragments. Life sciences, medical engineering, genetics, disease ...

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Problems solved by technology

Therefore, this flow effect can be used to carry out biochemical experiments, but the experiment in the microchannel has its own shortcomings, such as the poor mixing effect of the reagents in the microchamber, the large demand for samples, and the low sensitivity of the analysis. The process is caused by uneven mixing due to different fluid flow rates in the mixing channel, etc.

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