Dual colloidal gold test strip for detecting human bone metabolic biomarker and detection device applying same
A technology for colloidal gold test strips and metabolic markers, which is applied in the field of clinical medical testing, can solve the problems of long time-consuming detection technology, complicated operation, and high requirements for instruments and equipment, and achieves the effects of simple operation, accurate detection results, and improved detection sensitivity.
Inactive Publication Date: 2017-02-01
吕炜锋
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[0008] The purpose of the present invention is to address the deficiencies in the prior art, based on colloidal gold labeling technology and streptavidin-biotin amplification system, to provide a quantitative, fast, simple, high-sensitivity detection of human bone metabolism markers double colloid The gold test strip and the detection device using it can solve the problems of long time-consuming, complicated operation and high requirements for instruments and equipment in the existing detection technology
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[0025] The following description is only an illustration of the technical solutions claimed in the present invention, and is not any limitation to these technical solutions. The protection scope of the present invention shall be determined by the contents described in the appended claims.
[0026] Take human serum osteocalcin (OC), one of the markers of human bone metabolism, as an example. The present invention will be described in detail.
[0027] Monoclonal antibodies Anti-Osteocalcin antibody 1 (anti-OC mAb 1) and Anti-Osteocalcin antibody 2 (anti-OC mAb 2) targeting different sites of human serum osteocalcin were prepared in advance. Preparation of 41nm colloidal gold: Heat 100mL of 0.01% chloroauric acid solution to 95°C, quickly add 1mL of 1% trisodium citrate solution, and stir thoroughly. After the mixed solution turned red, continue heating for 15 min. Cool to room temperature after stopping heating, filter at 0.22 μm, make up the nano-gold solution to 100 mL with...
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The invention belongs to the field of clinical laboratory techniques and in particular relates to a colloidal gold test strip for quantitatively detecting human bone metabolic biomarker and a detection device applying same. The test strip provided by the invention comprises a sample pad, a glass fiber mat coated with gold-labeled streptavidin, a glass fiber mat coated with a gold-labeled antibody, a nitrocellulose membrane and an absorbent pad which are sequentially adhered with one another on a bottom plate in a staggered manner. A quality control line pre-enveloped with a goat anti-rat IgG antibody is formed in the nitrocellulose membrane; a detection line parallel to the quality control line is formed in the nitrocellulose membrane; an antibody capable of being specifically bound with a to-be-detected antigen human bone metabolic biomarker is coated on the detection line; the gold-labeled antibody is an antibody capable of being specifically bound with the to-be-detected antigen human bone metabolic biomarker. According to the test strip provided by the invention, the human bone metabolic biomarker can be quantitatively detected, the requirement on instruments is low, the time of clinically detecting the human bone metabolic biomarker is shortened, and the detection complexity and cost can be reduced.
Description
technical field [0001] The invention belongs to the field of clinical medical examination, and in particular relates to a colloidal gold test strip for quantitatively detecting human bone metabolism markers based on double colloidal gold probes and a detection device using the same. [0002] technical background [0003] The World Health Organization defines osteoporosis (Osteoporosis, OP) as low bone mineral content, changes in bone microstructure, easy to be destroyed, reduced bone toughness, and prone to fractures. There are more than 200 million patients with osteoporosis and its related complications and sequelae worldwide. The incidence rate of osteoporosis is increasing year by year. According to recent statistics, its global incidence rate ranks seventh among all chronic diseases. [0004] The most commonly used method for clinically diagnosing osteoporosis is bone mineral density (BMD) testing, and the most commonly used method is dual-energy X-ray absorptiometry (...
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IPC IPC(8): G01N33/543G01N33/558G01N33/577G01N33/68
CPCG01N33/558G01N33/54306G01N33/577G01N33/68G01N2800/108
Inventor 吕炜锋孙浩
Owner 吕炜锋
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