Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof

The technology of colloidal gold and detection card is applied in the field of immunocolloidal gold detection card of bisphenol A and its preparation, and achieves the effects of simple operation, easy popularization and use, and fast detection speed.

Active Publication Date: 2013-04-03
JIANGSU WISE SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of the above situation, in order to overcome the defects of the prior art, the purpose of the present invention is to provide a bisphenol A immune colloidal gold detection card and a preparation method thereof, which can effectively solve the problem of detecting the industrial compound environmental hormone bisphenol A quickly and easily. The problem

Method used

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  • Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof
  • Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof
  • Colloidal gold immunochromatographic card for detecting bisphenol A (BPA) and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] figure 1 It is the structural diagram of the immune colloidal gold detection card of bisphenol A of the present invention, figure 2 It is the cross-sectional structure diagram of the test strip in the immune colloidal gold detection card of bisphenol A of the present invention: among the figure 9 is a PVC glue board; 7 is a sample pad; 8 is a colloidal gold binding pad, and the colloidal gold binding pad is coated with Bisphenol A monoclonal antibody colloidal gold marker; 10 is the coated membrane, that is, nitrocellulose membrane, which is coated with bisphenol A-BSA and rabbit anti-mouse IgG; 11 is absorbent cotton, which is made of absorbent cotton Made of materials such as filter paper.

[0026] A sample pad 7 and a colloidal gold bonding pad 8 are adhered to one end (sample end) of the PVC rubber plate 9 , and the sample pad 7 is lapped on the colloidal gold bonding pad 8 .

[0027] Adhesive coating film 10 in the middle of PVC rubber plate 9. The rabbit anti-...

Embodiment 2

[0030] The preparation of bisphenol A colloidal gold detection card is realized by the following steps:

[0031] 1. Monoclonal antibody: After the hybridoma cell line was conventionally cultured and passaged in vitro, bisphenol A-BSA was injected into the peritoneal cavity of BALB / C mice, the ascites was collected, and the monoclonal antibody was purified by affinity chromatography;

[0032] 2. Preparation of colloidal gold solution: Take a 1 L Erlenmeyer flask, add 495 mL of ultrapure water, and then add 1% chloroauric acid (HAuCl 4 ·3H 2 O) 5 mL, prepared into 500 mL 0.01% chloroauric acid aqueous solution, heated to boil, then added 1% trisodium citrate (Na 3 C 6 h 5 o 7 2H 2 O) Solution 5-7 mL, continue to stir and heat, when the color of the solution turns completely transparent purple red, stop heating after maintaining for 5 minutes, add water to the original volume, cool to room temperature, and store at 2-8°C for later use;

[0033] 3. Antibody pretreatm...

Embodiment 3

[0041] Minimum detection limit test

[0042] Bisphenol A minimum detection limit test

[0043] Accurately weigh 0.500 g of bisphenol A standard substance, dissolve it in ultrapure water and dilute to 100 mL, mark it as A 1 solution, take A 1 Add 1 mL of the solution to a volumetric flask and add ultrapure water to 1000 mL to form A 2 solution, take A 2 Add 1 mL of the solution to a volumetric flask and add ultrapure water to 1000 mL to form A 3 solution, then A 3 The concentration of the solution is 5 ng / mL (5 ppb). take A 3 Put 1 mL of the solution in 4 test tubes, and label them 1~4 respectively, add 9 mL, 4 mL, 1 mL, and 0 mL of ultrapure water to the test tubes respectively, and prepare the bisphenol A standard solution with concentrations of 0.5 ng / mL, 1 ng / mL, 2.5 ng / mL, 5 ng / mL; take another empty test tube, add 5 mL ultrapure water, and mark it as No. 5 test tube. Take the liquid in the above test tubes 1-5 as samples and add them dropwise to the bisphenol A t...

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Abstract

The invention relates to a colloidal gold immunochromatographic card for detecting BPA and a preparation method of the colloidal gold, which belongs to the field of industrial compound environmental hormone detection technology. The test strip in a case of the colloidal gold immunochromatographic card is composed of a PVC slab, a sample cushion, a colloidal gold combination cushion, a coating film, and a water absorption cotton, wherein a colloidal gold film is a glass fiber film containing BPA monoclonal antibody; the coating film is a cellulose nitrate film and is provided with a T line and a C line; the T line is coated with a BPA protein conjugate; and the C line is coated with a rabbit antimouse IgG antibody. The colloidal gold immunochromatographic card provided by the invention is effective in detecting BPA quickly, and is simple, convenient, quick, and accurate.

Description

technical field [0001] The invention relates to the technical field of detection of industrial compound environmental hormones, in particular to an immune colloidal gold detection card for bisphenol A and a preparation method thereof. Background technique [0002] Bisphenol A (Bisphenol A, abbreviated as BPA) is a known endocrine disruptor (environmental hormone), an important chemical raw material, also known as polycarbonate in industry, mainly used in the production of epoxy resin, polycarbonate, Monomer of polysulfone resin; it can also be used as raw material for phenolic resin and plastic resin and antioxidant and stabilizer for rubber, plastic, pesticide, paint, ink, etc.; used in tableware, food packaging materials, plastic products, anti-corrosion coatings , Water transmission and distribution and water storage materials are widely used. Every year, 27 million tons of plastics containing BPA are produced worldwide. [0003] Bisphenol A can cause endocrine disor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74
Inventor 杜道林洪霞张祯薛永来
Owner JIANGSU WISE SCI & TECH DEV
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