Production method using food residues to prepare functional microorganism organic fertilizer
A technology of functional microorganisms and production methods, applied in the direction of microorganism-based methods, preparation of organic fertilizers, treatment of biological organic parts, etc., to achieve the effects of saving costs, promoting seed germination, and shortening time
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Embodiment 1
[0029] Collect fresh kitchen waste, separate solid and liquid to obtain solid waste and liquid waste, and pick out impurities such as broken glass and plastic in the solid waste.
[0030] 1. Preparation of Candida tropicalis bacterial liquid: Candida tropicalis (purchased from China Center for Type Culture Collection (CCTCC), its preservation number is: CCTCCAY92045) was obtained from Candida slant medium Pick one ring and inoculate it into the liquid Candida seed culture medium, culture at 28°C and 150 rpm for 24 hours with shaking, check the purity under the microscope, the strain is in the logarithmic growth phase, stop the culture, and obtain the first-grade strain of Candida tropicalis Then, according to the inoculation amount of 3% (v / v), it was inserted into the fermenter filled with Candida seed medium, the temperature was 28°C, the stirring speed was 250rpm, and the ventilation rate was 3L / (L·min). Time 24 hours, obtain Candida tropicalis bacterium liquid, described C...
Embodiment 2
[0041]Collect fresh kitchen waste, separate solid and liquid to obtain solid waste and liquid waste, and pick out impurities such as broken glass and plastic in the solid waste.
[0042] 1. Preparation of Candida tropicalis bacterial liquid: Pick a ring of Candida tropicalis from the Candida slant medium and inoculate it into the liquid Candida seed medium, at 30°C, 150rpm, shaking culture for 48 hours, microscopically check the purity, the strain is in the logarithmic growth phase, stop the culture, and obtain the first-class strain of Candida tropicalis; In the fermenter of the yeast seed culture medium, the temperature is 30°C, the stirring speed is 200rpm, the ventilation rate is 2L / (L·min), and the fermentation time is 24 hours, to obtain the Candida tropicalis bacteria liquid, the Candida tropicalis The slant medium contains glucose 5g / L, peptone 2g / L, yeast extract 1g / L, sodium chloride 0.5g / L, agar 20g / L, the balance is water, pH is 6.5, sterilization conditions: steri...
Embodiment 3
[0055] Collect fresh kitchen waste, separate solid and liquid to obtain solid waste and liquid waste, and pick out impurities such as broken glass and plastic in the solid waste.
[0056] 1. Preparation of Candida tropicalis bacterial liquid: Pick a ring of Candida tropicalis from the Candida slant medium and inoculate it into the liquid Candida seed medium, at 37°C, 200rpm, shaking culture for 12 hours, microscopically check the purity, the strain is in the logarithmic growth phase, stop the culture, and obtain the first-class strain of Candida tropicalis; In the fermentation tank of the yeast seed medium, the temperature is 35°C, the stirring speed is 300rpm, the ventilation rate is 4L / (L·min), and the fermentation time is 72 hours, to obtain the Candida tropicalis liquid, and the Candida slant The medium contains glucose 5g / L, peptone 2g / L, yeast extract 1g / L, sodium chloride 0.5g / L, agar 20g / L, the balance is water, pH is 7.5, sterilization conditions: sterilization at 115...
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