The invention discloses a seed treatment method for increasing the germination rate and improving the disease resistance of soybean seeds. The seed treatment method comprises the specific steps of seed pretreatment, preparation of seed soaking liquid, seed soaking, disease resistance treatment and sterilization treatment. The seed pretreatment comprises the following steps: putting mature soybean seeds into acidic soaking liquid with the temperature of 20 to 30 DEG C for 50 to 60 minutes, taking out the seeds, putting the seeds on sand paper, rinsing the seeds with clean water after wax on the outer skins of the seeds fall off, screening the seeds, and removing flat particles; the preparation of the seed soaking liquid comprises the following steps: adding the following components in weight proportion into a common aqueous liquid: 20 to 24g / L ammonium nitrate, 0.4 to 0.6g / L sodium phthalate, 0.3 to 0.5g / L sodium humate, 2 to 4mg / L nutrition adding liquid and 0.04 to 0.06mg / L gibberellin, and uniformly mixing for later use. The seed soaking liquid is enriched with nutritional elements, so that the soybean seeds can get through the period of dormancy within shorter time, the speed that the soybean seeds are germinated into seedlings is increased, irradiation of infrared achieves a sterilization effect; the seeds are soaked in potassium permanganate or formalin, the disease resistance of the seeds is enhanced, so that the culture success rate of the soybeans is increased, and high germination rate is realized.