Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof
A bio-organic fertilizer and fermentation liquid technology, applied in the field of agricultural biology, can solve problems such as low microbial content of products, failure to meet product quality requirements, single function of strains, etc., achieve good control effect, improve utilization rate, and improve fermentation level Effect
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Embodiment 1
[0025] Use a strain of Bacillus subtilis A that has no antagonistic effect on related strains to ferment alone, and its fermentation broth is mixed with 15% of Bacillus pumilus fermentation broth and 15% of Pseudomonas fluorescens fermentation broth at a ratio of 70% to prepare a fermentation broth combination The mixture is fully absorbed and mixed in the prepared organic fertilizer according to the ratio of 150-250mL fermentation broth composition / 1000g organic fertilizer to make functional bio-organic fertilizer.
[0026] The functional bio-organic fertilizer obtained in this example has antagonistic and growth-promoting effects on various plant soil-borne diseases.
Embodiment 2
[0028] 1. Separate fermentation of functional bacteria
[0029] Bacillus subtilis A (Bacillus subtilis) 1.1630, Bacillus subtilis B (Bacillus subtilis 1.1468), Bacillus pumilus 1.937 and Pseudomonas fluorescens GIM1.49 were purchased from Guangdong Institute of Microbiology Microorganism Collection Center.
[0030] The fermentation conditions of each strain are as follows:
[0031] 1) Bacillus subtilis A (Bacillus subtilis 1.1630)
[0032] Fermentation medium: starch 0.5% sucrose 0.8% bean cake powder 0.3% K 2 HPO 4· 3H 2 O 0.2% MgSO 4· 7H 2 O 0.06% Ammonium Sulfate 0.04% Calcium Carbonate 0.2% Yeast Extract 0.03% Culture Condition: pH 7.0~7.5 Temperature 30~31℃ Time 30~36h
[0033] 2) Bacillus subtilis B (Bacillus subtilis 1.1468)
[0034] Fermentation medium: starch 0.5% sucrose 0.3% bean cake powder 0.1% K 2 HPO 4· 3H 2 O 0.1% MgSO 4· 7H 2 O 0.1% Ammonium Sulfate 0.08% Calcium Carbonate 0.2% Yeast Extract 0.1% Culture Condition: pH 7.2~7.5 Temperature 30~34℃ Ti...
Embodiment 3
[0047] The organic fertilizer that embodiment 2 makes is used for following experiment:
[0048] 1. Application effect of functional bio-organic fertilizer on pepper
[0049] In this experiment, peppers were cultivated in field greenhouses, and the disease occurred for many years.
[0050] Fertilizer dosage and usage: 100kg / mu, used as base fertilizer when transplanting pepper seedlings (converted to 20g / plant).
[0051] Results: Most of the untreated control peppers withered and had no yield, while the treatment treated with bio-organic fertilizer basically grew normally without any disease.
[0052] 2. Application effect of functional bio-organic fertilizer on potted tomatoes:
[0053] This experiment is a potted plant in a net room, and the soil selected for the potted plant is vegetable garden soil with perennial disease.
[0054] Fertilizer dosage and usage: 14.3g / kg soil, base application when transplanting seedlings.
[0055] Results: 1) After 40 days of inoculation...
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