Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof

A bio-organic fertilizer and fermentation liquid technology, applied in the field of agricultural biology, can solve problems such as low microbial content of products, failure to meet product quality requirements, single function of strains, etc., achieve good control effect, improve utilization rate, and improve fermentation level Effect

Inactive Publication Date: 2009-06-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such products are still in the stage of market promotion, and there are still many problems in all links from product production to field application, especially in terms of product technology content, microorganisms are the technical core of products, and the screening and compou...

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  • Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Use a strain of Bacillus subtilis A that has no antagonistic effect on related strains to ferment alone, and its fermentation broth is mixed with 15% of Bacillus pumilus fermentation broth and 15% of Pseudomonas fluorescens fermentation broth at a ratio of 70% to prepare a fermentation broth combination The mixture is fully absorbed and mixed in the prepared organic fertilizer according to the ratio of 150-250mL fermentation broth composition / 1000g organic fertilizer to make functional bio-organic fertilizer.

[0026] The functional bio-organic fertilizer obtained in this example has antagonistic and growth-promoting effects on various plant soil-borne diseases.

Embodiment 2

[0028] 1. Separate fermentation of functional bacteria

[0029] Bacillus subtilis A (Bacillus subtilis) 1.1630, Bacillus subtilis B (Bacillus subtilis 1.1468), Bacillus pumilus 1.937 and Pseudomonas fluorescens GIM1.49 were purchased from Guangdong Institute of Microbiology Microorganism Collection Center.

[0030] The fermentation conditions of each strain are as follows:

[0031] 1) Bacillus subtilis A (Bacillus subtilis 1.1630)

[0032] Fermentation medium: starch 0.5% sucrose 0.8% bean cake powder 0.3% K 2 HPO 4· 3H 2 O 0.2% MgSO 4· 7H 2 O 0.06% Ammonium Sulfate 0.04% Calcium Carbonate 0.2% Yeast Extract 0.03% Culture Condition: pH 7.0~7.5 Temperature 30~31℃ Time 30~36h

[0033] 2) Bacillus subtilis B (Bacillus subtilis 1.1468)

[0034] Fermentation medium: starch 0.5% sucrose 0.3% bean cake powder 0.1% K 2 HPO 4· 3H 2 O 0.1% MgSO 4· 7H 2 O 0.1% Ammonium Sulfate 0.08% Calcium Carbonate 0.2% Yeast Extract 0.1% Culture Condition: pH 7.2~7.5 Temperature 30~34℃ Ti...

Embodiment 3

[0047] The organic fertilizer that embodiment 2 makes is used for following experiment:

[0048] 1. Application effect of functional bio-organic fertilizer on pepper

[0049] In this experiment, peppers were cultivated in field greenhouses, and the disease occurred for many years.

[0050] Fertilizer dosage and usage: 100kg / mu, used as base fertilizer when transplanting pepper seedlings (converted to 20g / plant).

[0051] Results: Most of the untreated control peppers withered and had no yield, while the treatment treated with bio-organic fertilizer basically grew normally without any disease.

[0052] 2. Application effect of functional bio-organic fertilizer on potted tomatoes:

[0053] This experiment is a potted plant in a net room, and the soil selected for the potted plant is vegetable garden soil with perennial disease.

[0054] Fertilizer dosage and usage: 14.3g / kg soil, base application when transplanting seedlings.

[0055] Results: 1) After 40 days of inoculation...

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Abstract

The invention discloses a fermentation liquor composition used for preparing microbial organic fertilizer, which comprises the components based on the weight percentage: 60-70% of bacillus subtilis fermentation liquor, 15-20 % of bacillus pumilus fermentation liquor and 15-20 % of pseudomonas fluorescens fermentation liquor. The invention also provides a preparation method of the fermentation liquor composition, which comprises: bacillus subtilis, bacillus pumilus and the pseudomonas fluorescens are respectively fermented separately, and then the obtained fermentation liquors are mixed. The strain of the fermentation liquor composition has optimal combination, so as to realize versatility. The selected strain not only has effect of growth promotion, but also has antagonism effect for multiple plant soil-borne diseases. The bacillus subtilis and the pseudomonas fluorescens in the product are plant rhizosphere growth-promoting bacteria which can stimulate and induce the systemic resistance of the crops, improve the self immunity and enhance disease resistance.

Description

technical field [0001] The invention relates to a fermented liquid composition for preparing bio-organic fertilizer, a preparation method and application thereof, and belongs to the field of agricultural biotechnology. Background technique [0002] Bio-organic fertilizer refers to a type of fertilizer that combines the effects of microbial fertilizers and organic fertilizers, which is composed of specific functional microorganisms and organic materials that are mainly derived from animal and plant residues and have been harmlessly treated and decomposed. Compared with ordinary organic fertilizers, the technical content of bio-organic fertilizer production is relatively high. In addition to adding biological agents to promote the decomposition and decomposition of organic materials in the process of decomposing, in order to achieve the purpose of directional decomposing, etc., the products also need Microorganisms with specific functions are added to enhance the effect of the...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/125C12R1/08C12R1/39
Inventor 肖相政廖宗文
Owner SOUTH CHINA AGRI UNIV
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