A strain of Bacillus pumilus and its application

A technology of Bacillus pumilus and bacteria, which is applied in the application field of microbial technology and achieves the effect of significant antagonism

Active Publication Date: 2022-06-28
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are certain limitations in the prevention and control of these two diseases.

Method used

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  • A strain of Bacillus pumilus and its application
  • A strain of Bacillus pumilus and its application
  • A strain of Bacillus pumilus and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1, the acquisition of Bacillus pumilus 154-2

[0043] 1. Soil source

[0044] Soil of East Wheat Field in Wanglaoba Village, Yuanzhou District, Guyuan City, Ningxia

[0045] 2. Screening of strains

[0046] (1) Soil sample collection

[0047] Z-shaped 5-point sampling method for soil sample collection: collect 200g soil samples at each point, mix the soil samples evenly, and put 200g of soil samples into a sterilization bag by quartering method as a soil sample. Three soil samples were collected from each plot as replicates. Record the time, place and type of sampling. The collected soil samples were stored in a refrigerator at 4°C for bacterial isolation.

[0048] (2) Isolation of bacteria

[0049] Plate dilution method: Weigh 10 g of soil sample into a conical flask, add 90 mL of sterile water, then shake at 200 rpm and 28 °C in a shaker, take it out after 20 min and let it stand at room temperature for 10 min to prepare a soil bacteria suspension stock ...

Embodiment 2

[0058] Example 2 Identification of 16S rRNA gene of Bacillus pumilus 154-2

[0059] Extracting the genomic DNA of strain 154-2,

[0060] Using primers: 27F 5'-AGAGTTTGATCCTGGCTCAG-3' and 1492R5'-TACGGCTACCTTGTTACGACTT-3', using the extracted DNA as a template, PCR amplification was performed to obtain the target fragment. The PCR reaction system is:

[0061] Table 1 Taq polymerase chain reaction system

[0062]

[0063]

[0064] The basic conditions of PCR reaction were: pre-denaturation at 94°C for 3 min, denaturation at 94°C for 30s, annealing at 56°C for 30s, extension at 72°C for 90s (1 kb / min), pre-extension at 72°C for 8 min, and storage at 4°C for a total of 30 cycles. After the reaction, the PCR products were tested by 1% agarose gel electrophoresis, and the results were detected and recorded by a gel imager. The gel electrophoresis results of the 16S rRNA gene were as follows: image 3 shown, image 3 1 represents the Marker; 2 represents the product of the 16...

Embodiment 3

[0067] Example 3 Physiological and biochemical identification of Bacillus pumilus 154-2

[0068] The physiological and biochemical characteristics of Bacillus pumilus 154-2 of the present invention are: can secrete β-galactosidase, 3-hydroxybutanone to produce acetomethyl carbinol and gelatinase; cannot hydrolyze arginine, lysine, ornithine Acid, sodium citrate and sodium thiosulfate, etc.; cannot oxidize glucose, mannitol, rhamnose and sucrose, etc.; can utilize 20 carbon sources such as L-arabinose, ribose, D-xylose, fructose and mannose Acid production, see Tables 2 and 3.

[0069] Table 2 Physiological and biochemical characteristics of strain 154-2 – enzyme activity, carbon source oxidation

[0070]

[0071]

[0072] +: positive reaction; -: negative reaction;

[0073] Table 3 Physiological and biochemical characteristics of strain 154-2 - acid production using carbon source

[0074]

[0075]

[0076] +: positive reaction; -: negative reaction; W: weak posi...

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Abstract

The invention relates to a strain of bacillus pumilus and its application. The Bacillus pumilus strain has been preserved in the China Center for Type Culture Collection on August 24, 2020. The preservation name is: Bacillus pumilus 154-2, and the preservation number is CCTCC M 2020446. The Bacillus pumilus of the present invention has a significant inhibitory effect on rice bacterial blight, and at the same time has a significant inhibitory effect on rice bacterial stripes, banana bacterial wilt, cowpea wilt, pepper spot fungus, walnut bacterial black spot and wheat red spot. Mildew bacteria also exhibit broad-spectrum antibacterial activity, which has important application prospects in biocontrol.

Description

technical field [0001] The invention belongs to the application field of microorganism technology, in particular to a strain of Bacillus pumilus and its application. Background technique [0002] Rice is one of the main food crops in my country, the planting area accounts for about 1 / 4 of the country's arable land, and the annual output accounts for about 1 / 2 of the country's total grain output (Abdullah AB., Ito S., Adhana K. Estimate of rice consumption in Asian countries and the world towards 2050. Proceedings for Workshop and Conference on Rice in the World at Stake [R]. 2006.), rice is also an important strategic resource for food security and sustainable agricultural development in my country. Two pathogenic species of Xanthomonas oryzae, X.oryaze pv.oryaze(Xoo) and X.oryaze pv.oryzicola(Xoc), can infect rice and cause bacterial leaf blight (BLB) and rice Bacterial leaf streak (BLS). The existence and development of these two diseases seriously affect the high and sta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P1/00C12R1/07
CPCA01N63/22
Inventor 邹丽芳黄梦桑李逸朗杨瑞环闫依超方园陈功友
Owner SHANGHAI JIAOTONG UNIV
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