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Pseudomonas fluorescens and application thereof in promoting growth of poplar

A technology of Pseudomonas fluorescens and poplar, applied in the field of biological fertilizers and microbial fertilizers, can solve the problems of screening and application research that have not been reported, and achieve the effects of excellent strain resources, strong dissolution effect, and promotion of growth and development

Active Publication Date: 2009-11-11
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the screening and application of high-efficiency phosphorus-solubilizing bacteria in the poplar rhizosphere

Method used

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  • Pseudomonas fluorescens and application thereof in promoting growth of poplar
  • Pseudomonas fluorescens and application thereof in promoting growth of poplar
  • Pseudomonas fluorescens and application thereof in promoting growth of poplar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: JW-JS1 Phosphorus Solubilization Ability Test:

[0019] Phosphate Solubilizing Medium A: Glucose 10g, Ca 3 (PO 4 ) 3 5g, MgCl 2 5g, KCL 0.2g, MgSO 4 ·7H 2 O 0.25g, (NH 4 ) 2 SO 4 0.1g, distilled water 1000mL, pH 7.0.

[0020] Phospholytic medium B: with Ca 10 (PO 4 ) 6 (OH) 2 Instead of Ca in phosphate-dissolving medium A 3 (PO 4 ) 3 , the other components and contents are the same.

[0021] Inoculate the activated JW-JS1 strain into NB medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2-7.4), shake culture at 30°C for 18-24h to make seed liquid, and take 0.5ml of seed solution were inoculated in 100mL Erlenmeyer flasks containing 50mL of phosphate-dissolving medium A and 50mL of phosphate-dissolving medium B, and the same volume of blank seed solution was used as the control (CK), and each treatment was repeated 3 times, 30 °C, 180r / min shaking culture for 72h, the fermentation broth was centr...

Embodiment 2

[0023] Example 2: JW-JS1 optimal phosphorus solution test:

[0024] Using NBRIP (International Institute of Botany Phosphate Growth Medium, formulated with Phosphorus Solubilizing Medium A) as the basic medium, change the C source, N source, different C / N and environmental conditions. The results show that the optimum conditions for phosphorus solubilization are as follows: in a 100mL Erlenmeyer flask, glucose is used as carbon source, ammonium sulfate is used as nitrogen source, C / N is 80 / 1-100 / 1, liquid volume is 20-30mL, initial pH value is 7.0 ~7.2, inoculum size 3%~4%, temperature 28℃~30℃.

Embodiment 3

[0025] Embodiment 3: JW-JS1 greenhouse pot test:

[0026] After activating JW-JS1, use an inoculation loop to pick a small amount of bacteria and inoculate it into a 100mL Erlenmeyer flask containing 50mL of NB medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2-7.4), 29 Cultivate with shaking at 180r / min for 72h. Centrifuge the fermentation broth (4°C, 6000r / min) for 5min, rinse the bacteria with sterile normal saline for 2 to 3 times, then adjust the bacterial suspension with sterile normal saline (7~8×10 8 cfu / mL) to make bacterial agent. NL-895 cutting seedlings and black poplar seedlings (60 days old) were inoculated respectively, and the same amount of sterile saline was used as the control, and the inoculation volume was 15 mL / plant for cutting seedlings and 5 mL / plant for seedlings. 6 replicates per treatment were placed in the greenhouse for unified management, the light was 12h / day, and watering was timely.

[0027]...

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Abstract

The invention discloses pseudomonas fluorescens separated from rooting zone soil and capable of dissolving difficultly soluble inorganic phosphorus in high efficiency. The pseudomonas fluorescens is classification-named as pseudomonas fluorescens JW-JS1 and has been stored in the China Center for Type Culture Collection, the storage No. is M 209027, and the storage data is Feb. 18, 2009. The invention also discloses application of the pseudomonas fluorescens in promoting growth of poplar. Under the condition of liquid shaking culture, JW-JS1 strains have stronger effect of dissolving tricalcium phosphate and hydroxyapatite; and the pseudomonas fluorescens JW-JS1 can be inoculated to cuttage seedlings of NL-895poplar and tree seedlings of eastern cottonwood. Results show that the pseudomonas fluorescens obviously promotes the growth of the poplar. Therefore, the invention provides a desirable strain resource for developing a phosphorus-dissolving bacteria fertilizer special for the poplar.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a Pseudomonas fluorescens that efficiently decomposes phosphorus in the rhizosphere of poplars and its application in promoting the growth of poplars. Background technique [0002] Phosphorus is one of the essential macronutrient elements that limit the growth and development of plants, but more than 95% of the phosphorus in the soil and the Ca in the soil 2+ , Fe 2+ , Fe 3+ 、Al 3+ and other metal ions combined to lose plant effectiveness. Most of the soluble phosphorus fertilizers applied to the soil are quickly fixed by specific adsorption and chemical precipitation, and the utilization rate in the current season is only 5% to 10%. In addition to the after-effects of crops, the utilization rate generally does not exceed 25%. Phosphate fertilizers accumulate in the soil as insoluble phosphates, which are diffic...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/39
Inventor 吴小芹刘辉叶建仁
Owner NANJING FORESTRY UNIV
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