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Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1

A methylotrophic and Bacillus technology, applied in botany equipment and methods, plant protection, plant growth regulators, etc., can solve problems such as endangering human health, destroying ecological balance, pesticide environmental pollution, etc., and achieve environmental pollution-free , no pesticide residue effect

Active Publication Date: 2016-05-11
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term and large-scale application of chemical pesticides leads to pesticide residues and environmental pollution, which eventually endangers human health and destroys the ecological balance. At the same time, it also makes pathogens highly resistant to various chemical fungicides

Method used

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  • Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1
  • Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1
  • Plant bacteriostasis method adopting bacillus methylotrophicus strain NKG-1

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0093] Preparation of the fermentation and culture filtrate of methylotrophic bacillus. The methylotrophic bacillus was inoculated into the LB medium at a ratio of 1:100, and oscillated at 37°C and 220r / min for 24h. Collect the fermentation broth, centrifuge (10000r / min, 4°C), filter and sterilize the supernatant with a 0.22 μm filter membrane to obtain a sterile fermentation broth.

[0094] Using the mycelial growth rate method to detect the effects of antagonistic Bacillus on tomato gray mold, tomato leaf mold, rice blast, pepper anthracnose, apple ring stalk, apple rot, cucumber anthracnose, wheat sheath blight, and gibberella wheat The influence of the test pathogenic fungi on the growth of the pathogenic fungus, asparagus stem rot, corn leaf spot, cotton wilt, grape black rot, tobacco brown spot, peach rhizopus soft rot, and cotton seedling blight.

[0095] Under aseptic conditions, take 10 mL of the prepared Bacillus aseptic fermentation broth and 90 mL of the melted PDA medi...

Embodiment 1

[0104] Example 1: Antibacterial method of grape black rot

[0105] Picking out the marginal hyphae cultured in opposition, microscopic observation found that the antibacterial effect of NKG-1 on grape black rot is mainly the effect on hyphae and spores. Various metabolites of NKG-1 (including antibacterial proteins, bacteriocins, antibiotics, various cell wall degrading enzymes, etc.) destroy the structure of the mycelial wall of pathogenic bacteria, causing hyphal enlargement, distortion, distortion, and disintegration of the mycelial wall. The spores constricted in the middle, the cell wall was dissolved, and the protoplasm leaked out.

[0106] The antibacterial effect of methylotrophic Bacillus NKG-1 on grape black rot is as follows Picture 10 Shown.

[0107] Table 2. The inhibitory effect of methylotrophic bacillus NKG-1 on the hyphae of grape black rot

[0108]

[0109] (1) Measurement results of the effect of the fermentation broth of methylotrophic Bacillus NKG-1 strain on th...

Embodiment 2

[0126] Example 2: Antibacterial method of asparagus stem rot

[0127] The antibacterial effect of methylotrophic Bacillus NKG-1 on asparagus stem rot bacteria is as follows Figure 17 Shown.

[0128] Table 5. The inhibitory effect of methylotrophic Bacillus NKG-1 on the hyphae of asparagus stem rot

[0129]

[0130] Effect of Methylotrophic Bacillus NKG-1 on Controlling Asparagus Stalk Rot in Greenhouse

[0131] The field experiment has 3 treatments: A. Biocontrol agent NKG-1500 times liquid; B. 25% metalaxyl wettable powder 600 times liquid; C. Blank control. 14m per cell 2 , There are 3 rows of isolation areas between the plots, and each treatment is arranged in a completely random block group, repeated 3 times, and a total of 9 plots are tested. Before the test, the plots were divided according to the test plan, and the isolation ditch was opened. The use amount of 15000mL 1×10 per hectare 7 cfu / mL biocontrol agent NKG-1. The biocontrol agent NKG-1500 times liquid is sprayed once...

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Abstract

The invention discloses a plant bacteriostasis method adopting the bacillus methylotrophicus strain NKG-1. Pesticide is prepared from bacillus methylotrophicus strain NKG-1 bacteria liquid, and the prepared pesticide methylotrophicus agentia is applied in the growing period of plants to inhibit germs on the plants. By the adoption of the plant bacteriostasis method adopting the strain, various pathogenic fungi and bacteria can be inhibited, a good control effect is realized, and therefore application prospects are broad.

Description

Technical field [0001] The invention relates to a plant antibacterial method implemented by using methylotrophic bacillus NKG-1, in particular to a plant antibacterial method implemented using methylotrophic bacillus (Bacillusmethylotrophicusstrain NKG-1), which can effectively inhibit pathogenic bacteria. Infestation. Background technique [0002] Plant diseases can cause serious economic losses to agricultural production. At present, pesticides are usually applied to control plant diseases and insect pests at various growth stages. Among them, chemical pesticides are mainly applied, which have the characteristics of convenient fertilization and quick effect. However, long-term large-scale application of chemical pesticides leads to pesticide residues and environmental pollution, which ultimately endangers human health and destroys the ecological balance. At the same time, pathogens have become severely resistant to various chemical fungicides. The use of living microorganisms ...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00A01P3/00A01G13/00
CPCA01G13/00A01N63/00
Inventor 葛蓓孛张克诚刘炳花刘艳赵文珺辛辛诺
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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