Method for preparing liquid organic fertilizer through fermentation of aquatic product waste

A technology of aquatic product leftovers and organic fertilizers, applied in the direction of organic fertilizers, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of large-scale application of liquid organic fertilizers, high power consumption and labor costs, and production costs Advanced problems, to achieve the effect of low cost, increased energy consumption, and reduced production costs

Inactive Publication Date: 2016-06-22
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic hydrolysis method needs to add expensive protease; the fermentation method needs to invest in the purchase of expensive fermentation equipment, and the power consumption and labor costs of the fermentation process are relatively high
Regardless of the enzymatic hydrolysis method or fermentation method, the production cost is very high, and the prepared liquid organic fertilizer is difficult to apply on a large scale

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: The thermostable facultative anaerobic bacteria Bacillusmethylotrophicus HL-4 capable of producing protease preserved on the slant was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h to prepare BacillusmethylotrophicusHL- 4 seed solutions for later use. The fresh tilapia leftovers are minced and set aside. Add 30 kg of ground tilapia scraps and 100 kg of water into the enzymolysis tank, and add 100 g of glucose, 820 g of calcium chloride, 750 g of ferrous chloride, 450 g of chloride Zinc and 750 grams of dipotassium hydrogen phosphate to obtain a fermentation medium; add 8 kilograms of Bacillus methylotrophicus HL-4 seed liquid to the fermentation medium in the enzymolysis tank; control the fermentation temperature at 45°C to 60°C during 0h to 20h, and control it after 20h The fermentation temperature is 60°C-75°C, the rotation speed is 50rpm-100rpm, and the fermentation time is 40h; after the fermenta...

Embodiment 2

[0024] Example 2: The thermostable facultative anaerobic bacteria Bacillusmethylotrophicus HL-4 capable of producing protease preserved on the slant was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h to prepare BacillusmethylotrophicusHL- 4 seed solutions for later use. Mince the head of the fresh vannamei shrimp and set aside. Add 35 kg of minced shrimp heads and 100 kg of water into the enzymatic hydrolysis tank, and add 50 grams of glucose, 530 grams of food-grade calcium chloride, 450 grams of ferrous chloride, and 350 grams of zinc chloride into the enzymatic hydrolysis tank and 650 grams of dipotassium hydrogen phosphate to obtain a fermentation medium; add 8 kilograms of Bacillus methylotrophicus HL-4 seed liquid to the fermentation medium in the enzymolysis tank; control the fermentation temperature at 45°C to 60°C from 0h to 20h, and control the fermentation after 20h The temperature is 60°C-75°C, the rotation...

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PUM

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Abstract

The invention discloses a method for preparing liquid organic fertilizer through fermentation of aquatic product waste. The method comprises main steps as follows: fresh aquatic product waste minced; the minced aquatic product waste is added to an enzymolysis tank and nutrients and water are added and mixed evenly to be used as a fermentation medium; the enzymolysis tank is inoculated with high-temperature-resistant facultative anaerobe methyl nutritional type Bacillus methylotrophicus HL-4 capable of producing protease for simultaneous enzymolysis and fermentation; a fermented broth is filtered for precipitation removal, and thus the liquid organic fertilizer can be prepared. Enzymolysis and fermentation processes can be finished in the enzymolysis tank simultaneously, equipment and operation are optimized, the production cost of the liquid organic fertilizer is significantly reduced, and the method has good application prospect.

Description

technical field [0001] The invention relates to a method for preparing liquid organic fertilizer by fermenting leftovers of aquatic products, belonging to the field of processing and utilization of aquatic products. Background technique [0002] Amino acid is the basic unit of protein and is an organic compound containing nitrogen. Amino acid can not only be directly absorbed by humans and animals, but also can be absorbed and utilized by plants. It is an excellent organic nutrient. Amino acid liquid fertilizer is a liquid fertilizer whose nutrient composition is mainly amino acid, so that plants can directly absorb and utilize it. As nutrients, amino acids can be absorbed by crops faster than inorganic nitrogen fertilizers, which can increase crop yield, improve quality, and reduce pesticide residues. [0003] Fish protein liquid fertilizer is an organic liquid fertilizer prepared by enzymatically degrading low-value fish and adding certain trace elements and active substa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C05F1/00C12R1/07
CPCC05F1/002C05F1/005C12P21/06Y02W30/40Y02A40/20
Inventor 洪鹏志刘唤明周春霞杨萍夏杏洲
Owner GUANGDONG OCEAN UNIVERSITY
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