Bacillus methylotrophicus UTM401 and applications thereof

A technology of bacillus and methyl nutrition, applied in the application, bacteria, fungicides and other directions, to achieve the effect of low production cost, excellent performance and inhibition of plant pathogenic bacteria

Active Publication Date: 2012-10-03
大地绿源环保科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this microorganism has the characteristics of degrading starch and its application in the fermentation treatment of organic waste, and the preparation of crop growth-promoting functional biological fertilizers has not been publicly reported so far.

Method used

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  • Bacillus methylotrophicus UTM401 and applications thereof
  • Bacillus methylotrophicus UTM401 and applications thereof
  • Bacillus methylotrophicus UTM401 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Isolation and Identification of Methylotrophic Bacillus UTM401

[0033] Weigh 10g of sludge decomposed compost sample (collected from the compost fermentation workshop of Dadi Lvyuan Environmental Protection Technology (Beijing) Co., Ltd.) and place it in a 100ml sterile water 250ml triangular flask with 10 small glass beads, and shake it at a constant temperature at 40°C Shake on the bed for 1 hour, then let it stand for 30 minutes, in the ultra-clean bench, absorb 1ml of the supernatant, and transfer it to 100ml of sterilized acclimation medium (3g of beef extract, 3g of yeast extract, 8g of peptone, chloride Sodium 3g, soluble starch 10g, 1000ml distilled water), enrichment and acclimatization culture for 3 days on a constant temperature shaker at 40°C with a rotation speed of 160r / min. In the same way, draw 1ml of the enrichment solution again, and subculture for another 3 generations under the same conditions.

[0034] Take 1mL of the acclimatized bacter...

Embodiment 2

[0037] The preparation of embodiment 2 strain UTM401 fermented seed liquid (1)

[0038] Transfer the slant surface of the production strain UTM401 of the present invention stored at 4°C to the NA medium plate, cultivate it at 30°C until it is thick, and then use a sterile glass spatula to transfer all of it to a 5000ml triangle containing 1000ml LB liquid medium In the bottle, culture on a shaker at 37°C with a rotation speed of 160r / min for 2 days. At this time, the OD 600 2.202, stop the cultivation, this is the primary fermentation seed liquid.

[0039] The primary fermentation seed liquid was inoculated into a fermentation seed tank containing LB liquid medium at 5% of the volume ratio of the liquid medium for fermentation. The fermentation conditions are temperature 37°C, intermittent stirring and ventilation, stirring once every 1 hour, stirring for 5 minutes each time, stirring speed 150r / min, ventilation once every half an hour, 5 minutes each time, ventilation rate 1...

Embodiment 3

[0041] The preparation of embodiment 3 strain UTM401 fermented seed liquid (2)

[0042] Transfer the slant surface of the production strain UTM401 of the present invention stored at 4°C to the NA medium plate, cultivate it at 30°C until it is thick, and then use a sterile glass scraper to transfer all of it to a 5000ml triangle containing 2000ml LB liquid medium In the bottle, culture on a shaker at 30°C with a rotation speed of 150r / min for 3 days. At this time, the OD 600 It is 2.084, stop cultivating, this is the primary fermentation seed liquid.

[0043] The primary fermentation seed liquid was inoculated into the fermentation seed tank containing LB liquid medium according to 10% of the volume ratio of the liquid medium to carry out fermentation culture. Fermentation conditions are temperature 30°C, intermittent stirring and ventilation, stirring once every 1 hour, stirring for 10 minutes each time, stirring speed 120r / min, ventilation once every half an hour, 5 minutes ...

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Abstract

The invention provides bacillus methylotrophicus UTM401 and applications thereof. The biological collection number of the bacillus methylotrophicus UTM401 is CGMCC No.5927. The bio-fertilizer can be prepared by inoculating the fermentation seed liquid of the strain UTM401 into an organic waste bank and fermenting. By adopting the strain UTM401 to prepare the bio-fertilizer, the cost is low, the quality is stable, and the fermentation period can be shortened; moreover, the bacteria are increased quickly again in solid material, and a stable bacterial colony system is formed with a fermentation material carrier; and high-density colonization of the plant rhizosphere can be realized, and the effects of suppressing phytopathogen and harmful microorganisms of rhizosphere, promoting the plant growth and improving the crop yield are realized. The strain UTM401 and the bio-fertilizer prepared from the fermentation organic waste thereof are green and environment-friendly and pollution-free, and have good performance and relatively high economic and social benefits.

Description

technical field [0001] The invention relates to the field of biological fertilizers, in particular to a methylotrophic bacillus strain UTM401 and its use for preparing biological fertilizers. Background technique [0002] Biological fertilizers use the properties of microorganisms to provide nitrogen nutrients needed for crop growth or increase the availability of refractory mineral nutrients in the soil, thereby promoting the absorption and growth of nutrients by crops, and improving their yield and quality. Biological fertilizers are irreplaceable in the development of ecological agriculture. The use of organic waste to prepare biological fertilizers can not only treat organic waste and solve environmental problems, but also realize the resource utilization of organic waste. It is a recognized, low-cost, and effective way to recycle resources efficiently. [0003] At present, the biological fertilizers on the market are either due to the degradation of the production stra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/02A01P1/00A01P3/00A01P21/00C12R1/07
Inventor 刘永跃周顺桂许宜北汪涌
Owner 大地绿源环保科技(北京)有限公司
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