Biocontrol microorganism of wide spectrum antagonistic plant pathogenic fungi and application thereof
A technology of plant pathogenic fungi and biocontrol microorganisms, applied in the direction of plant growth regulators, applications, biocides, etc., to achieve low cost and high control efficiency
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[0015] 1. The cultivation of Mo-Bm and the preparation of bacterial agent in the present invention
[0016] Mo-Bm strain test tube slant seed culture: put the thalline of this bacterial strain on the slant of beef extract peptone solid medium (beef extract 5g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, agar 20g, pH 7.2-7.4), Obtain slant seeds after culturing at 15-40°C for 1-5 days, and the suitable culturing condition is culturing at 28°C for 2 days.
[0017] Liquid culture of Mo-Bm strain liquid seeds: inoculate the slanted seeds into the beef extract peptone liquid medium (beef extract peptone solid medium without adding agar) in the triangular flask, shake the table under the condition of 15-40°C and 50-350rpm Culture for 2-7 days. The optimum culture conditions are 28°C, 200rpm and culture 3.
[0018] Mo-Bm bacterial strain fermenter is cultivated in large quantities: liquid seed is inserted into the PDB in the fermenter by the ratio of 1-5% (V / V) (peeled potato 200...
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