Biocontrol microorganism of wide spectrum antagonistic plant pathogenic fungi and application thereof

A technology of plant pathogenic fungi and biocontrol microorganisms, applied in the direction of plant growth regulators, applications, biocides, etc., to achieve low cost and high control efficiency

Inactive Publication Date: 2011-07-13
YUNNAN UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The antagonistic properties of this microorganism against p

Method used

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Embodiment Construction

[0015] 1. The cultivation of Mo-Bm and the preparation of bacterial agent in the present invention

[0016] Mo-Bm strain test tube slant seed culture: put the thalline of this bacterial strain on the slant of beef extract peptone solid medium (beef extract 5g, peptone 10g, NaCl 5g, agar 20g, water 1000mL, agar 20g, pH 7.2-7.4), Obtain slant seeds after culturing at 15-40°C for 1-5 days, and the suitable culturing condition is culturing at 28°C for 2 days.

[0017] Liquid culture of Mo-Bm strain liquid seeds: inoculate the slanted seeds into the beef extract peptone liquid medium (beef extract peptone solid medium without adding agar) in the triangular flask, shake the table under the condition of 15-40°C and 50-350rpm Culture for 2-7 days. The optimum culture conditions are 28°C, 200rpm and culture 3.

[0018] Mo-Bm bacterial strain fermenter is cultivated in large quantities: liquid seed is inserted into the PDB in the fermenter by the ratio of 1-5% (V / V) (peeled potato 200...

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Abstract

The invention relates to a biocontrol microorganism of wide spectrum antagonistic plant pathogenic fungi and application thereof, belonging to the technical field of microbial pesticides. The biocontrol microorganism is a Bacillus methylotrophicus Mo-Bm strain preserved in a CGMCC (China General Microbiological Culture Collection Center) on 12th October 2010 with a preservation number of CGMCC No.4207. A 16S rRNA gene sequence (with a sequence number of HQ325853 in a GeneBank database) of the Mo-Bm strain is a main characteristic basis for identifying the strain; and the Mo-Bm strain can resist 0.5-2 percent (V/V) carbinol, and is a secondary characteristic basis. The microorganism and metabolins thereof are prepared into a Mo-Bm wettable powder biocontrol anti-bacteria agent together with pesticide conventional absorption carriers and aids. The microorganism has a control effect of above 70 percent on tobacco black shank, cruciferclubroot and pseudo-ginseng root rot. The biocontrol microorganism has the characteristics of low cost, high control effect, no residue and safety to people and livestock, and is an efficient biological pesticide for controlling fungus diseases during organic agriculture planting.

Description

Technical field: [0001] The invention relates to a biocontrol microorganism capable of broad-spectrum antagonizing plant pathogenic fungi and its application, belonging to the technical field of microbial pesticides. Background technique: [0002] The harm of plant pathogenic fungi to planting agriculture is immeasurable. There are 28 types of tobacco fungal diseases found in China, and the economic loss caused by them accounts for 37.8% of the tobacco diseases, among which the tobacco black shank disease caused by the invasion of Phytophthoranicotianae is the most serious (Zhu Mingliang, Lei Liping, 2008). As the main production area of ​​my country's famous and precious Chinese medicinal material Panax notoginseng, Yunnan accounts for more than 90% of the country's output. In 2009, the income of Panax notoginseng agriculture was 1.69 billion yuan. The annual incidence of root rot of Panax notoginseng caused by Fusarium spp. and other pathogenic bacteria is generally 5% to...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00
Inventor 莫明和林碧莲黄英周峰
Owner YUNNAN UNIV
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