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Wettable powder of bacillus methylotrophicus and preparation method thereof

A methylotrophic, wettable powder technology, applied in botany equipment and methods, biocides, disinfectants, etc., can solve the problems of inconvenient use and inability to store for a long time, achieve broad application prospects, facilitate long-term storage, good control effect

Active Publication Date: 2013-04-17
陕西恒田生物农业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the disadvantages that the fermentation products of methylotrophic bacillus can not be placed for a long time and are inconvenient and used, the present invention provides a wettable powder of methylotrophic bacillus

Method used

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  • Wettable powder of bacillus methylotrophicus and preparation method thereof
  • Wettable powder of bacillus methylotrophicus and preparation method thereof
  • Wettable powder of bacillus methylotrophicus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1 prepares the wettable powder of methylotrophic bacillus of the present invention

[0026] The formula is: 70 kg of the original powder of methylotrophic bacillus, 34 kg of light calcium carbonate, 2.5 kg of sodium dodecylbenzenesulfonate, 1 kg of potassium dihydrogen phosphate, 1.5 kg of dipotassium hydrogen phosphate, dispersant 3 kg of sodium methylbismethyl naphthalene sulfonate.

[0027] Preparation method: Mix the above raw materials evenly, control the processing temperature at about 25°C, pulverize in a jet mill, and pass through a 150-mesh sieve to obtain the product.

[0028] The former powder of described methylotrophic bacillus is prepared by the following method:

[0029] (1) Inoculate the methylotrophic bacillus with the preservation registration number of the present invention as CGMCC No.6793 into LB liquid culture medium shake flask, shake and cultivate to logarithmic growth phase; the LB liquid culture medium is yeast extract 5g , peptone...

Embodiment 2

[0033] Embodiment 2 The wettable powder of the methylotrophic bacillus of the present invention prevents and treats the test of citrus canker

[0034]For two consecutive years from 2010 to 2011, research on the control effect of the methylotrophic Bacillus wettable powder prepared in the above-mentioned Example 1 on citrus canker was carried out in Baoshan Town, Chenggu County, Shaanxi Province. The area of ​​the test orchard is 4hm2, the size is 2m×3m, the terrain is flat, the drainage and irrigation are convenient, the soil fertility is medium, and other management measures such as fertilizer, water and pest control are the same. Spray the medicine for the first time on the afternoon of August 31, 2010 and 2011. At this time, the autumn shoots have been drawn out by 5 to 8 cm. Use a sprayer to evenly spray the crown of the tree until the front and back of the leaves and the branchlets are soaked. On the afternoon of September 15th, the medicine was sprayed for the second tim...

Embodiment 3

[0043] Embodiment 3 The original powder and wettable powder of the methylotrophic bacillus of the present invention are used to prevent and treat citrus leaf canker

[0044] For two consecutive years from 2010 to 2011, research on the control effect of the methylotrophic bacillus powder and wettable powder prepared in the above embodiment 1 on citrus leaf canker was carried out in Baoshan Town, Chenggu County, Shaanxi Province. The area of ​​the test orchard is 4hm2, the size is 2m×3m, the terrain is flat, the drainage and irrigation are convenient, the soil fertility is medium, and other management measures such as fertilizer, water and pest control are the same. Spray the medicine for the first time on the afternoon of August 31, 2010 and 2011. At this time, the autumn shoots have been drawn out by 5 to 8 cm. Use a sprayer to evenly spray the crown of the tree until the front and back of the leaves and the branchlets are soaked. On the afternoon of September 15th, the medici...

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Abstract

The invention relates to a wettable powder of bacillus methylotrophicus. The wettable powder is composed of raw powder of bacillus methylotrophicus that is prepared with a specific method, light calcium carbonate, sodium dodecyl benzene sulfonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate and 2-3 parts by weight of a dispersant methylene dimethyl naphthalene sodium sulfonate. Furthermore, the invention relates to a use of the wettable powder in controlling citrus diseases, in particular citrus canker.

Description

technical field [0001] The present invention relates to biopesticides, in particular to a wettable powder with the fermentation product of methylotrophic bacillus as an active ingredient, and a preparation method for the wettable powder, in particular to the wettable powder for preventing and treating citrus ulcer disease application. technical background [0002] Citrus canker is a worldwide disease caused by rod-shaped bacteria that endangers the growth of citrus. It is the subject of plant quarantine at home and abroad. It was confused with citrus scab in the early stage. It was not described as 2 distinct diseases until 1913, and the causative bacteria were isolated and identified in 1915. The disease originated in Asia, and it is most common in Asian countries, so it is also called Asian disease. The disease is distributed in Guangdong, Guangxi, Fujian, Taiwan, Jiangxi, Zhejiang, Jiangsu, Hunan, Hubei, Guizhou, Sichuan, Shaanxi and other provinces in China. The patho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01N25/14A01N25/22A01P1/00
Inventor 唐满仓沈小英马玉明安德荣
Owner 陕西恒田生物农业有限公司
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