Bacillus methylotrophicus and application thereof on aspect of preventing and controlling fusarium graminearum

A methylotrophic and Bacillus technology, applied in the biological field, can solve the problems of increased difficulty in control and the lack of ideal high-resistant wheat varieties, etc., and achieve the effect of good application prospects

Inactive Publication Date: 2015-02-11
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] For many years, the control of wheat head blight has mainly relied on chemical pesticides, but with the environmental problems caused by chemical pesticides, coupled with years of drug use has led to the emergence of pathogenic bacteria resistant Fusarium gramin...

Method used

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  • Bacillus methylotrophicus and application thereof on aspect of preventing and controlling fusarium graminearum
  • Bacillus methylotrophicus and application thereof on aspect of preventing and controlling fusarium graminearum
  • Bacillus methylotrophicus and application thereof on aspect of preventing and controlling fusarium graminearum

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Experimental program
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Effect test

Embodiment 1

[0024] The isolation and identification of embodiment 1 bacteria

[0025] 1. Isolation of bacteria

[0026] (1) In June 2012, 10 ears of diseased wheat ears collected in Jiangsu were placed in 100 ml sterile distilled water for 15 minutes to prepare a bacterial suspension in an ultra-clean workbench, and then treated in a water bath at 80°C for 10 minutes.

[0027] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, The strains with different colors and transparency were streaked and purified on the plate, and the purified strains were applied to the confrontation culture experiment of Fusarium graminearum. The obtained strain with strong antagonistic ability to Fusarium graminearum was named JS20GA.

[0028] 2. Identification

[0029] (1) According to the method described...

Embodiment 2

[0039] Example 2 Antagonism of Methylotrophic Bacillus JS20GA to Fusarium graminearum

[0040] The antagonism of methylotrophic Bacillus JS20GA to Fusarium graminearum was detected by confrontation culture method.

[0041] 1. Use a hole puncher with a diameter of 5mm to pick out a plate from the edge of the cultured pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG1 colony, and pick the plate into the center of the PDA plate with a sterilized toothpick.

[0042] 2. Inoculate the same volume and concentration of JS20GA at a distance of 25mm from the center of the PDA plate with a four-dot dash line. Take the PDA plate inoculated only with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is any antibacterial effect bands formed. Do three parallels for each group. The results are shown in Table 1:

[0043] Table 1 The inhibitory effect of JS20GA on Fusarium graminearum after 5 days of culture

[004...

Embodiment 3

[0046] The inhibitory effect of embodiment 3JS20GA fermented liquid to Fusarium graminearum

[0047] The antagonism of methylotrophic Bacillus JS20GA to Fusarium graminearum was detected by confrontation culture method.

[0048] 1. Use a hole puncher with a diameter of 5mm to pick out a plate from the edge of the cultured pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG1 colony, and pick the plate into the center of the PDA plate with a sterilized toothpick.

[0049] 2. Inoculate JS20GA with the same volume and concentration at four points 25mm away from the center of the PDA plate. Take the PDA plate inoculated only with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is any antibacterial effect. bands formed. Do three parallels for each group. The results are shown in Table 2. It shows that JS20GA has a strong inhibitory effect on Fusarium graminearum.

[0050] Table 2 The inhibitory effect o...

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Abstract

The invention discloses Bacillus methylotrophicus and an application of the Bacillus methylotrophicus on the aspect of preventing and controlling fusarium graminearum. The preservation number of the Bacillus methylotrophicus disclosed by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 9500, and the nucleotide sequence of the Bacillus methylotrophicus is shown as SEQ ID No. 1. The bacterial strain is used for the application of biological prevention and control in plant diseases caused by the fusarium, wheat and corns serve as biological prevention and control materials for crop diseases; no matter new biological pesticide or biological prevention and control fungicide is developed, the bacterial strain has an excellent application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a methylotrophic bacillus and its application in preventing and treating Fusarium graminearum. Background technique [0002] Fusarium graminearum (Fusarium graminearum) is one of the important pathogens of gramineous crops, which can cause wheat head blight (Fusarium Head Blight, FHB). Fusarium graminearum is a major factor limiting wheat yield (Dubin, 1997) . my country's climatic conditions are very favorable for the occurrence of wheat head blight, and the perennial disease in the middle and lower reaches of the Yangtze River caused a yield loss of 10%-15%, and a production reduction of nearly 50% in a pandemic year. Due to the impact of global warming and changes in farming systems such as straw returning to the field and no-tillage cultivation, the occurrence area of ​​wheat head blight in my country has rapidly expanded from the Yangtze River Basin to Northwest and...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/205C12R2001/07
Inventor 赵月菊刘阳邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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