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Antibacterial agent special for potatoes as well as preparation method thereof

An anti-bacteria and potato technology, applied in the agricultural and biological fields, can solve the problems of improper control, great influence on potato quality, and intolerant storage of diseased potatoes.

Active Publication Date: 2018-06-08
SHENZHEN BATIAN ECOTYPIC ENG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After the occurrence of scab, although the lesion is limited to the cortex, the diseased potato is not resistant to storage and has an ugly appearance, which has a great impact on the quality of the potato, resulting in a decline in commodity value and serious economic losses. Generally, the production can be reduced by 10%-30%. Block even cut production by more than 40%
Due to its characteristics of being transmitted by water and other means, improper or untimely control will cause greater catastrophic losses

Method used

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  • Antibacterial agent special for potatoes as well as preparation method thereof
  • Antibacterial agent special for potatoes as well as preparation method thereof
  • Antibacterial agent special for potatoes as well as preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] S1. Strain activation: Bacillus subtilis, methylotrophic Bacillus, Bacillus sporogenes, and Paecilomyces lilacinus microbial strains were respectively connected to the slope, and the medium was glucose 30g / L, starch 15g / L, and peptone 23g / L, NaCl 10g / L, KH 2 PO 4 0.5g / L, the rest is distilled water; pH7.0. Cultivate for 30 hours, then add each to sterile water and shake for 30 minutes, count with a hemocytometer after being uniform, so that the effective number of viable bacteria per ml of various bacteria is greater than 1×10 11 One, get the bacterial suspension.

[0025] S2. Seed tank fermentation: sterilize the first-level seed tank, put it into the medium and then sterilize it, insert the bacterial suspension, and pass it into sterile air for cultivation to obtain the first-level seed tank fermentation liquid; second-level seed tank sterilization , put into the culture medium and then sterilize, connect the first-level seed tank fermentation liquid to the second...

Embodiment 2

[0029] S1. Strain activation: eight species of Bacillus subtilis, methylotrophic Bacillus, Bacillus lateralis, Bacillus amyloliquefaciens, Bacillus licheniformis, Bacillus megaterium, Paecilomyces lilacinus, Paenibacillus jelly-like The microbial strains were connected to the slope respectively, and the medium was glucose 30g / L, starch 15g / L, peptone 23g / L, NaCl 10g / L, KH 2 PO 4 0.5g / L, the rest is distilled water; pH7.0. Cultivate for 48 hours, then add each to sterile water and shake for 25 minutes, count with a hemocytometer after being uniform, so that the effective number of viable bacteria per ml of various bacteria is greater than 1×10 11 One, get the bacterial suspension.

[0030] S2. Seed tank fermentation: sterilize the first-level seed tank, put it into the medium and then sterilize it, insert the bacterial suspension, and pass it into sterile air for cultivation to obtain the first-level seed tank fermentation liquid; second-level seed tank sterilization , put i...

Embodiment 3

[0034] S1. Strain activation: Bacillus subtilis, methylotrophic Bacillus, Bacillus sporogenes, and Paecilomyces lilacinus microbial strains were respectively connected to the slope, and the medium was glucose 30g / L, starch 15g / L, and peptone 23g / L, NaCl 10g / L, KH 2 PO 40.5g / L, the rest is distilled water; pH7.0. Cultivate for 42 hours, then add each into sterile water and shake for 30 minutes, count with a hemocytometer after being uniform, so that the effective number of viable bacteria per ml of various bacteria is greater than 1×10 11 One, get the bacterial suspension.

[0035] S2. Seed tank fermentation: sterilize the first-level seed tank, put it into the medium and then sterilize it, insert the bacterial suspension, and pass it into sterile air for cultivation to obtain the first-level seed tank fermentation liquid; second-level seed tank sterilization , put into the culture medium and then sterilize, connect the first-level seed tank fermentation liquid to the secon...

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Abstract

The invention provides an antibacterial agent special for potatoes. The antibacterial agent comprises bacillus subtilis and paecilomyces lilacinus; the weight ratio of the bacillus subtilis to the paecilomyces lilacinus is 6-9 to 1; the bacillus subtilis at least comprises three of bacillus laterosporus, bacillus methylotrophicus, bacillus licheniformis, bacillus megatherium, bacillus subtilis, paenibacillus mucilaginosus and bacillus amyloliquefaciens. The antibacterial agent special for the potatoes, which is prepared by the invention, can reasonably utilize interaction among microorganisms,has high adaptability, high stability and high pathogen antagonism, can efficiently antagonize pathogenic bacteria of potato early blight and sore coffee diseases, can stimulate plant growth and canimprove crop quality so as to fulfill the yield-increasing and high-yield aims.

Description

technical field [0001] The invention belongs to the fields of agriculture and biotechnology, and in particular relates to a special potato antibacterial agent, which is suitable for potato early blight and potato scab. Background technique [0002] Potatoes are one of the most important crops in my country, second only to rice, wheat and corn, and are the fourth largest crops in my country. Potatoes are grown in a wide range of areas, and diseases are relatively complex, among which potato early blight and potato scab caused by a variety of plant pathogenic Streptomyces are the most common and common diseases of potatoes. [0003] Potato blight bacteria not only harm potatoes, but also harm eggplant, pepper, tomato and so on. Early blight mainly damages the leaves, and also damages the petioles and stems when it is severe. When the leaves are infected, the lesions are off-white to dark brown, round or nearly round, and sometimes the development and expansion of the lesions...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A01N63/04A01N63/00A01P3/00A01P1/00C12R1/07C12R1/79C12R1/10C12R1/11C12R1/125
CPCA01N63/00A01N63/30C12N1/14C12N1/20
Inventor 张敏李艳萍邓进超罗嘉骏王宗抗邓宝元吴冬
Owner SHENZHEN BATIAN ECOTYPIC ENG
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