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Application of mycorrhizal fungi in large-scale plantation of gold thread lotus

A technology of mycorrhizal fungi and clematis, which is applied in the field of bioengineering and can solve problems such as the shortage of clematis resources

Inactive Publication Date: 2007-05-16
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method of using mycorrhizal fungus to cultivate A. output and quality, to solve the problem of shortage of clematis resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Cultivation of Mycena anoectochila MA-1 (Mycena anoectochila)

[0035] After activating MA-1 strains from the slant test tube stored at low temperature once, transfer them to PDA plates, and culture them at a constant temperature of 25°C for 10 days. Use a φ8mm hole puncher to punch holes on the edge of the colonies in the plates to form bacterial sheets, respectively. Into the liquid culture medium with small fragments for fermentation. The composition of the liquid culture medium is calculated by weight percentage: 2.5% of glucose, 0.15% of potassium dihydrogen phosphate, 0.1% of dipotassium hydrogen phosphate, 0.15% of magnesium sulfate, and 3% of wheat bran (cooked juice). The pH of the culture medium is 5.6. 250ml of the culture medium is packed in a 500ml Erlenmeyer bottle, and the MO-1 fungus is inserted after autoclaving. The culture condition is that the shaker shakes at a speed of 120 rpm, and after 14 days of dark culture at 23°C, the bacteria and ferment...

Embodiment 2

[0047] 1. Dendrobium MD-1 (Mycena dendrobii), Osmunda osmundicola MO-1 (Mycena osmundicola), Orchid osmundicola MO-2 (Mycena orchidicola) and Osmunda osmundicola. Cultivation of Mycena anoectochila MA-1 (Mycena anoectochila)

[0048] After activating MD-1, MO-1, MO-2 and MA-1 from the slant test tube strains preserved at low temperature, they were respectively transferred to PDA plates, and cultured at a constant temperature of 25°C for 20 days. Punch holes at the edge of the colonies in the plate to form bacterial flakes, and insert small fragments into the liquid medium for fermentation and culture. The composition of the liquid culture medium is calculated by weight percentage: 3% of glucose, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate, and 3% of wheat bran (cooked juice). The pH of the culture medium is 6.0. 200ml of culture medium is packed in a 500ml Erlenmeyer bottle, and the above four kinds of mycorrhizal fungi are respectively inserted after a...

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PUM

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Abstract

The invention relates to a method for using fungus root fungi in the large cultivation of gold thread lotus, wherein used four fungus root fungis are small mushroom pieces, as Mycena dendrobii, Mycena osmundicol, Mycena orchidicola, and Mycena anoectochila. And the method comprises that first cultivating fungus root fungi and gold thread lotus; when transplating the gold thread lotus into large field, adding fungus root fungi and gold thread lotus to be planted together. The invention can reduce the grow period of gold thread lotus, improve yield and the content of effective component.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a technical method for using mycorrhizal fungi in large-area cultivation of clematis to shorten the production cycle of clematis and improve the yield and quality of clematis. Background technique [0002] Clematis is a precious and rare Chinese herbal medicine. It is a perennial herb of the family Orchidaceae (Anoectochilus Blume). It is mainly distributed in Zhejiang, Fujian, Jiangxi, Guangdong, Guangxi, Hainan, and Guizhou in China. , Yunnan, Tibet, Taiwan and other provinces and regions. Clematis clematis is a whole herb used as medicine, which has the effects of clearing away heat and cooling blood, expelling wind and dampness, strengthening the heart, lowering blood pressure, detoxifying, relieving pain, and antitussive. Arthritis, infantile convulsions, bruises, snakebites and many other diseases have good curative effects. In recent years, clematis ha...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H3/00C12N1/14A01G1/00A01G7/00
Inventor 郭顺星于雪梅高微微陈晓梅王春兰孟志霞肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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