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Method for producing edible fungus mycorrhizal seedling of mycorhiza

An edible fungus and production method technology, applied in the directions of botanical equipment and methods, applications, fertilizer mixtures, etc., can solve the problems of high cost, cumbersome operation process, low efficiency, etc., and achieve high efficiency, simple and effective operation, and remarkable economy. effect of benefit

Inactive Publication Date: 2009-12-09
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide an improved production method of mycorrhizal edible fungus mycorrhizal seedlings, which can overcome the disadvantages of complicated operation process, high cost and low efficiency in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0022] The cultivation of embodiment 2 Tricholoma matsutake mycorrhizal seedlings

[0023] (1) Mycelium culture

[0024] The strains used in this experiment came from Yunnan. The mother plant was isolated from mycorrhizal tissue, and the modified MMN agar medium (Marx, 1969) (where wort juice and beef juice + peptone were removed, FeCl 3 (1% solution) to 2-3ml). Cultivate in a culture room (20±2°C), transfer tubes for 2 cultivations, and then apply or store in a refrigerator at 3±2°C for later use. Put the above-mentioned strains into the modified MMN liquid medium, put them on a shaker (120-130 rpm) and continue to cultivate for 15 days, then they will meet the cultivation requirements, and then they can be used to inoculate host plants.

[0025] (2) Preparation of ectomycorrhizal synthetic substrate (substrate 1)

[0026] Sterilize sepiolite ore powder (200 mesh, containing 25% sepiolite), humus soil (commercially available), and perlite (commercially available) at a tem...

Embodiment 3

[0032] The cultivation of embodiment 3 delicious boletus and masson pine mycorrhizal seedlings

[0033] (1) Mycelium culture

[0034] The mother plant was isolated from the fruiting body tissue purchased from the Yunnan market, and the modified MMN agar medium (Marx, 1969) (wherein wort juice and beef juice + peptone were removed, FeCl 3 (1% solution) added to 2~3ml) cultured in the culture room (20±5°C), subcultured once every 1~2 months, transferred to the tube for 2 cultures, and then applied or placed in 3±2°C Store in the refrigerator for later use. Put the above strains into the modified MMN liquid medium, put them on a shaker (120-130 rpm) and continue to cultivate for 7 days, then they will meet the cultivation requirements, and then they can be used to inoculate host plants.

[0035] (2) Preparation of ectomycorrhizal synthetic matrix

[0036] Sterilize sepiolite ore powder (200 mesh, containing 25% sepiolite), humus soil (commercially available), and perlite (comm...

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Abstract

The invention relates to a method for producing the edible fungus mycorrhizal seedling of a mycorhiza. The method is characterized by comprising the following steps: the mycelium of a target mycorrhizal fungus and the mycorhiza of a non-competitive mixed fungus are synthesized into a culture medium, are uniformly mixed, are spread in a container and are cultured under a condition which is suitable for the growth of hypha, wherein the surface of the container is disinfected by a potassium permanganate solution or sterilized under high temperature and high pressure, and the lower part of the container is spread with a layer of non-competitive mixed fungi or media sterilized under high temperature and high pressure; and a sterile germinated host plant seed of which the surface is disinfected and the culture medium culturing the target hypha are mixed and cultured under a proper ecological condition until the mycorhiza of a tree seedling root is formed. The synthesis experiment of the edible fungus mycorrhizal seedlings of various mycorhiza shows that the method has simple and effective operation and high efficiency, is very suitable for popularization and application in mass production, can be used for producing mycorrhizal seedlings at any time in one year and has obvious economic benefits.

Description

Technical field: [0001] The present invention relates to the technical field of edible fungi, specifically a method for producing mycorrhizal seedlings for the purpose of producing mycorrhizal edible fungus fruiting bodies; 1. A method for producing mycorrhizal seedlings for the purpose of improving plant resistance. Background technique: [0002] At present, the edible fruiting bodies of most of mycorrhizal edible fungi are well-known edible fungi, such as Tricholoma matsutake, Tubermelanosporum Vitt., Tuber magnatum, pine milk Mushroom (Lactarius deliciosus), Russet Lactaria (L.hatsudake), Delicious Boletus (Boletus edulis), Orange Capped Amanita (Amatanita caesarea), Chinese Truffle (Tuber sinense K.Tao et Liu), Indian Chunk Tuberindicum Cooke et Massee, Cantharellus cibarius Fr., Thelephora ganbajun Zang, Clavulinopsis miyabeana (Ito.) Ito., Suillus luteus ( L.: Fr.) Gray), Suillusgreviller (KL.) sing.), Russula virescens (schaeff.) Fr.), Amanita caesarea (Scop: Fr. )...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G3/00
Inventor 付绍春谭琦谭著明尚晓冬
Owner SHANGHAI ACAD OF AGRI SCI
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