Blueberry mycorrhizal fungi (coprinus micaceus) and separation method and application thereof

A mycorrhizal fungus and blueberry technology, applied in the field of microorganisms, can solve problems such as soil damage, reduce the damage of pests, and improve the effect of resistance

Inactive Publication Date: 2011-10-19
LIAONING ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention relates to a kind of blueberry mycorrhizal fungus and its separation method and application. Its purpose is to solve the problem of soil damage

Method used

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  • Blueberry mycorrhizal fungi (coprinus micaceus) and separation method and application thereof
  • Blueberry mycorrhizal fungi (coprinus micaceus) and separation method and application thereof
  • Blueberry mycorrhizal fungi (coprinus micaceus) and separation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] A method for separating blueberry mycorrhizal fungi, which is characterized in that the mycorrhizal fungi are obtained from the rhizosphere of wild bilberry plants, and the steps are as follows:

[0085] (1) Washing: Take fresh and complete roots of healthy wild blueberries, rinse them with tap water for one hour, then rinse with sterile water 3 times, and transfer the roots to sterile glass plates for later use.

[0086] (2) Disinfection: Put the roots to be used in step (1) in 70% alcohol solution for 30 seconds, rinse with sterile water 3 times, and then disinfect with 0.1% mercury solution for 2 minutes, rinse 3 times, and then immediately put it into a 1% by mass sterile sodium sulfide aqueous solution for 2 minutes, rinse with sterile water 3 times, and absorb the sterile filter paper for use.

[0087] (3) Cultivation: Cut the roots to be used after disinfection in step (2) into 0.8cm-long sections, place them on a PDA medium plate, invert the culture at a constant tempe...

Embodiment 2

[0093] A method for separating blueberry mycorrhizal fungi, which is characterized in that the mycorrhizal fungi are obtained from the rhizosphere of wild bilberry plants, and the steps are as follows:

[0094] (1) Washing: Take fresh and complete roots of healthy wild blueberries, rinse them with tap water for one hour, then rinse with sterile water twice, and transfer the roots to a sterilized glass dish for later use.

[0095] (2) Disinfection: Put the roots to be used in step (1) in 70% alcohol solution for 20 seconds, rinse with sterile water 3 times, and then disinfect with 0.1% mercury solution for 1 min, rinse 3 times, and then immediately put it in a 1% by mass sterile sodium sulfide aqueous solution for 3 minutes, rinse with sterile water 3 times, and absorb dry with sterile filter paper for later use.

[0096] (3) Cultivation: Cut the roots to be used after disinfection in step (2) into 0.5cm-long sections, place them on a PDA medium plate, and invert the culture at a cons...

Embodiment 3

[0102] A method for separating blueberry mycorrhizal fungi, which is characterized in that the mycorrhizal fungi are obtained from the rhizosphere of wild bilberry plants, and the steps are as follows:

[0103] (1) Washing: Take fresh and complete roots of healthy wild blueberries, rinse them with tap water for one hour, then rinse with sterile water 4 times, and transfer the roots to a sterilized glass plate for later use.

[0104] (2) Disinfection: Put the roots to be used in step (1) into 70% alcohol solution for 40 seconds, rinse with sterile water 3 times, and then disinfect with 0.1% mercury solution for 3 minutes, rinse 3 times, and then immediately put it into 1% by mass sterile sodium sulfide aqueous solution for 1 min, rinse with sterile water 3 times, and absorb dry with sterile filter paper for later use.

[0105] (3) Cultivation: Cut the to-be-used roots in step (2) into 1 cm long sections, place them on a PDA medium plate, invert the culture at a constant temperature at...

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Abstract

The invention relates to a blueberry mycorrhizal fungi (coprinus micaceus) and a separation method and application thereof, belongs to the technical field of microbiology, and mainly relates to a mycorrhizal fungi which separated from a Changbai Mountain wild cowberry root system for blueberry inoculation and application of the mycorrhizal fungi at promoting the growth of blueberry and improving the yield of the blueberry. The blueberry mycorrhizal fungi is preserved in the Chinese General Microbiological Culture Collection Center, and the classification name is Coprinus micaceus. By the application of the mycorrhizal fungi, the growth of the blueberry root system can be promoted. The invention has the advantages that the yield, fruit setting rate and insect damage prevention function can be improved, and the like.

Description

1. Technical Field: [0001] The invention belongs to the technical field of microorganisms, and mainly relates to a plant mycorrhizal fungus isolated from the roots of blueberry plants of the genus Vaccinium and its isolation method and its application in promoting the growth of blueberries at various stages, preventing insect pests and preventing cold damage. 2. Background technology: [0002] Plant mycorrhizal fungus refers to the mutual symbiosis formed by plant roots and fungi, namely "mycorrhiza" (mycorrhizas), which is an extremely common life activity and ecological phenomenon. Frank first coined the term "mycorrhiza" in 1885, and it has been in a period of steady development for several years. Until the 1950s, mycorrhizae entered a period of rapid co-development with the aid of modern molecular biology and biochemical technology. In the past 30 years, with the continuous establishment of new methods and technologies, the research content has become more extensive, and the ...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P21/00A01P7/04C12R1/645
Inventor 陶承光杨涛肇莹肖军王娜龚娜陈珣王红贾东贝杨镇
Owner LIAONING ACAD OF AGRI SCI
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