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Synthetic method of truffle and bacteriorhiza

A technology of mycorrhizae and seeds, applied in the biological field, can solve the problems such as no Indian truffles, and achieve the effect of optimizing the cultivation

Inactive Publication Date: 2008-12-24
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports and records on the synthetic method of Indian truffle (truffle) and Pinus huashanensis mycorrhiza in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Indian truffle (Chinese truffle) and Huashan pine mycorrhizal seedling synthesis method:

[0035] 1. Huashan pine seedling cultivation:

[0036] (1) Preparing species

[0037] Collect mature Huashan pine seeds in autumn and store them in the refrigerator at 4-8°C. The storage period does not exceed 1 year. Take out before use, 30% H 2 o 2 Soak for 2 hours, stir several times during the process → rinse several times with sterile water → discard floating seeds → soak in sterile water for 48 hours, remove → use mechanical means to slightly break the seeds → reuse 75% alcohol cotton gauze to wipe and disinfect , put in a petri dish for later use.

[0038] (2) Matrix

[0039] Mix vermiculite (not sieved) and perlite at a volume ratio of 1 / 1, add tap water to make the water content reach 30%, and sterilize under high pressure steam at 121-126°C for 1 hour; if it is newly produced vermiculite and perlite It can also be used directly without sterilization.

[0040] (3) S...

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PUM

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Abstract

The invention provides a method for synthesizing Chinese truffles and mycorhiza of China pinus montana. The steps related are as follows: filtration of China pinus montana seeds and truffle seed entities; culture of aseptic seedlings; preparation of microbial inoculum; arrangement, optimal selection and inoculation of culture substrates; culture of mycorrhizal seedlings; anatomization of mycorhiza forms; detection and confirmation of molecules and transplanting culture, and finally, the culture of truffles is realized.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and in particular relates to a synthesis method of Indian truffles (truffles sinensis) and pine mycorrhiza. Background technique: [0002] Truffles (truffles) belong to the ectomycorrhizal fungi (edible ectomycorrhizal fungi) that grow in the soil of pine, oak and other forests. Because they are rich in protein and other nutrients, special aphrodisiac compounds and human immune system regulating substances, they become Green and ecological functional food with high protein, low fat, and dietary therapy is popular in Europe and even the world. It is known as the "black diamond" in the kitchen and is the most expensive natural food in the world so far. my country exports more than 360 tons of Indian truffles (Chinese truffles) every year, and it has become a new type of wild mycorrhizal edible fungus for poverty alleviation and prosperity in mountainous forest areas. And more and more are generally w...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12R1/645
Inventor 刘培贵王向华于富强邓晓娟陈娟耿丽英田霄飞
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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