Application of mycorrhizal fungi in large-sclae plantation of dendrobium stem

A mycorrhizal fungus, a large-scale technology, applied in the field of bioengineering, can solve the problems of high cultivation survival rate, shortage of medicinal resources of Dendrobium, and high reproduction rate of Dendrobium

Inactive Publication Date: 2007-05-16
INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a kind of method of applying mycorrhizal fungi to cultivate Dendrobium in a large area. The method has a high reproduction rate of Dendrobium and a high survival rate of cultivation, can shorten the production cycle of cultivated Dendrobium, improve the output and quality of Dendrobium, and solve the problem of medicinal use of Dendrobium. The problem of shortage of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Cultivation of Osmunda osmundicola MO-1 (Mycena osmundicola)

[0039] After the MO-1 was activated once from the slant test tube strains preserved at low temperature, it was transferred to a PDA plate, and cultured at a constant temperature of 25°C for 18 days. The colony edge of the plate was punched with a φ8mm hole punch to form a bacterial sheet, respectively. Into the liquid culture medium with small fragments for fermentation. The composition of the liquid culture medium is calculated by weight percentage: 2.5% of glucose, 0.15% of potassium dihydrogen phosphate, 0.1% of dipotassium hydrogen phosphate, 0.15% of magnesium sulfate, and 3% of wheat bran (cooked juice). The pH of the medium is 5.6, and 250mL of medium is packed in a 500mL Erlenmeyer bottle, and the MO-1 fungus is inserted after autoclaving. The culture condition is that the shaker shakes at a speed of 120 rpm, and after 25 days of dark culture at 23°C, the bacteria and fermented products are crush...

Embodiment 2

[0051] 1. The cultivation of dendrobium MD-1 (Mycena dendrobii) and orchid mushroom MO-2 (Mycena orchidicola). In the PDA plate, culture at a constant temperature of 25°C for 10 days, use a φ8mm hole puncher to punch holes on the edge of the colony in the plate to form bacterial flakes, and insert small pieces into the liquid medium for fermentation and culture. The composition of the liquid culture medium is calculated by weight percentage: 3% of glucose, 0.3% of potassium dihydrogen phosphate, 0.15% of magnesium sulfate, and 3% of wheat bran (cooked juice). The pH of the medium is 6.0. 200mL of the medium is packed in a 500mL Erlenmeyer bottle, and MD-1 and MO-2 fungi are respectively inserted after autoclaving. The culture conditions were shaker shaking speed 100 rpm, dark culture at 25°C for 10 days, as the seed strains of MD-1 and MO-2 fungi.

[0052] The seed strains of MD-1 and MO-2 fungi cultured in liquid were inserted into solid medium for cultivation. The composit...

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Abstract

The invention relates to a method for using fungus root fungi in the large cultivation of gold thread lotus, wherein used four fungus root fungis are small mushroom pieces, as Mycena dendrobii, Mycena osmundicol, Mycena orchidicola, and Mycena anoectochila. And the method comprises that first cultivating fungus root fungi and gold thread lotus; when transplanting the gold thread lotus into large field, adding fungus root fungi and gold thread lotus to be planted together. The invention can reduce the grow period of gold thread lotus, improve yield and the content of effective component.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a practical technique for shortening the production cycle of dendrobium and improving the yield and quality of dendrobium by using mycorrhizal fungi in large-area cultivation of dendrobium. Background technique [0002] Dendrobium is a traditional precious Chinese medicine in my country. It has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, moistening the lungs and relieving cough. Pharmacological studies have shown that Dendrobium plants have the functions of enhancing the body's immunity, anti-tumor, anti-aging, and anti-platelet aggregation. At present, there are more than 30 kinds of Dendrobium plants for medicinal use in my country, which are mainly distributed in South China and Southwest China. The Pharmacopoeia of the People's Republic of China (2005 edition) contains Dendrobium nobile Lindl., Dendro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01H3/00C12N1/14A01G1/00A01G7/00
Inventor 郭顺星陈晓梅宋经元王春兰孟志霞肖培根
Owner INST OF MEDICINAL PLANT DEV CHINESE ACADEMY OF MEDICAL SCI
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