Large-scale propagation technology for mycorrhiza fungi inoculum

A technology of mycorrhizal fungi and arbuscular mycorrhizal fungi, applied in horticulture, botanical equipment and methods, gardening tools/equipment, etc., can solve the problem of large initial dosage of bacterial agents, inconvenient transportation and low bacterial agent production efficiency, etc. problems, to achieve the effect of easy real-time adjustment, convenient detection and large-scale production, and small footprint

Active Publication Date: 2013-06-05
INST OF SOIL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pot culture method is the main method for the propagation of AMF bacteria agents. The advantages are simple operation and reliable method. The disadvantages are that open management is easy to pollute, the culture period is long, the output of propagules is small, and it takes up a lot of space, which is not easy to transport and carry.
The traditional AMF bacterial agent production adopts the ratio of bacterial agent:sand:soil=1:1:1 to plant host plants for propagation. The initial amount of bacterial agent is large, and the production efficiency of the bacterial agent is low, which is difficult to meet the market demand.

Method used

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  • Large-scale propagation technology for mycorrhiza fungi inoculum
  • Large-scale propagation technology for mycorrhiza fungi inoculum
  • Large-scale propagation technology for mycorrhiza fungi inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] step 1:

[0030] Bacteria agent: select the number of spores ≥ 37.3 / cm 3 Glomus mosseae inoculant.

[0031] The sterilized soil is sandy loam soil after sterilization operation: the soil is taken from the surface layer (0-20cm) of cultivated soil without industrial and mining enterprises and pollution-free in the surrounding 20 kilometers. The soil used meets the first-class standard of "Soil Environmental Quality Standard" (GB65618-1995): cadmium ≤ 0.20mg / kg, mercury ≤ 0.15mg / kg, arsenic ≤ 15mg / kg, copper ≤ 35mg / kg, lead ≤ 35mg / kg , chromium≤90mg / kg, zinc≤100mg / kg, nickel≤40mg / kg, HCH≤0.05mg / kg, DDT≤0.05mg / kg. The soil fertility indicators used are: pH6.75, organic matter 1.45%, EC0.067mS / cm, available nitrogen 120mg / kg, Olsen-P17.7mg / kg, available potassium 129mg / kg, exchangeable calcium 1370mg / kg, exchangeable magnesium 164mg / kg, available boron 1.23mg / kg, available iron 5.28mg / kg, available manganese 25.6mg / kg, available copper 1.77mg / kg, available zinc 3.13mg / kg, ...

Embodiment 2

[0047] step 1:

[0048] Bacteria: select the number of spores to be 44.3 / cm 3 Glomus intraradices fungus.

[0049] The sterilized soil is sandy loam soil after sterilization operation: the soil is taken from the surface layer (0-20cm) of cultivated soil without industrial and mining enterprises and pollution-free in the surrounding 20 kilometers. The soil used meets the first-class standard of "Soil Environmental Quality Standard" (GB65618-1995): cadmium ≤ 0.20mg / kg, mercury ≤ 0.15mg / kg, arsenic ≤ 15mg / kg, copper ≤ 35mg / kg, lead ≤ 35mg / kg , chromium≤90mg / kg, zinc≤100mg / kg, nickel≤40mg / kg, HCH≤0.05mg / kg, DDT≤0.05mg / kg. The soil fertility indicators used are: pH6.75, organic matter 1.45%, EC0.067mS / cm, available nitrogen 120mg / kg, Olsen-P17.7mg / kg, available potassium 129mg / kg, exchangeable calcium 1370mg / kg, exchangeable magnesium 164mg / kg, available boron 1.23mg / kg, available iron 5.28mg / kg, available manganese 25.6mg / kg, available copper 1.77mg / kg, available zinc 3.13mg / kg...

Embodiment 3

[0065] step 1:

[0066] Bacteria agent: select the number of spores ≥ 37.3 / cm 3 The number of fungi and spores of Glomusmosseae was 44.3 / cm 3 The Glomus intraradices fungal agent, the mass ratio of the Glomus intraradices fungal agent and the Glomus intraradices fungal agent is 1:1.

[0067] The sterilized soil is sandy loam soil after sterilization operation: the soil is taken from the surface layer (0-20cm) of cultivated soil without industrial and mining enterprises and pollution-free in the surrounding 20 kilometers. The soil used meets the first-class standard of "Soil Environmental Quality Standard" (GB65618-1995): cadmium ≤ 0.20mg / kg, mercury ≤ 0.15mg / kg, arsenic ≤ 15mg / kg, copper ≤ 35mg / kg, lead ≤ 35mg / kg , chromium≤90mg / kg, zinc≤100mg / kg, nickel≤40mg / kg, HCH≤0.05mg / kg, DDT≤0.05mg / kg. The soil fertility indicators used are: pH6.75, organic matter 1.45%, EC0.067mS / cm, available nitrogen 120mg / kg, Olsen-P17.7mg / kg, available potassium 129mg / kg, exchangeable calcium 13...

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Abstract

The invention discloses a high-efficient large-scale propagation technology for mycorrhiza fungi inoculum, and belongs to the field of bio-fertilizer productions. The high-efficient large-scale propagation technology suitable for mycorrhiza fungi includes material selection, production process, quality control and product formation. Materials for the technology are easy to obtain, operation is easy and convenient and cost is low. Spore density of the propagated mycorrhiza fungi inoculum can reach more than fifty per cubic centimeter, and can even reach more than one hundred per cubic centimeter, and propagation efficiency is more than four times higher as compared with conventional propagation technology efficiency. The device adopted in the technology is high in flexibility, easy to be regulated in real time and small in occupation. Detection of product quality of the mycorrhiza fungi and large-scale production thereof are facilitated. An AMF (abuscular mycorrhizal fungi) propagation technology, high-efficient and prone to large-scale production, formed in the technology, is beneficial to application of the AMF in agricultural production, and new approaches for sustainable development for agriculture are provided.

Description

technical field [0001] The invention belongs to the technical field of biological fertilizer production, and in particular relates to a large-scale propagation technology of mycorrhizal fungal agents. Background technique [0002] (1) Under the pressure of energy crisis, resource shortage, environmental pollution and other pressures, the commercialization of mycorrhizal fungi products has attracted much attention [0003] Biological fertilizers play an irreplaceable role in improving crop quality, protecting the ecological environment and sustainable development of agriculture. Especially under the pressure of energy crisis, shortage of resources, and environmental pollution, the research and application of biofertilizers have attracted more attention from countries all over the world than ever before. [0004] Arbuscular mycorrhizal fungi (AMF) play a key role in improving plant stress and disease resistance and maintaining plant health. Arbuscular mycorrhizal fungi are a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G7/00
Inventor 梁林洲沈仁芳董晓英田凤娇
Owner INST OF SOIL SCI CHINESE ACAD OF SCI
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