A method for preventing bacterial wilt and soil compaction using vegetable oil and vegetable oil conditioner

Abstract

The invention discloses a method for preventing and controlling tobacco bacterial wilt by using vegetable oil and preventing soil compaction and a vegetable oil conditioner thereof, belonging to the technical field of bacterial wilt disease prevention and control. The vegetable oil conditioner is used for prevention and control. The vegetable oil conditioner comprises the following raw materials: vegetable oil, eggshell powder, sodium citrate, whey protein, schizonepeta oil, peanut shell powder, L-methionine and riboflavin. The vegetable oil conditioner is added to prevent and control tobacco bacterial wilt, and the vegetable oil conditioner is used to change soil animal activity, so as to solve the problem of soil compaction and poor air permeability caused by long-term use of vegetable oil in preventing and controlling tobacco bacterial wilt.

Description

Technical Field

[0001] The invention relates to the technical field of bacterial wilt disease prevention and control, and in particular to a method for preventing bacterial wilt disease and soil compaction by using vegetable oil and a vegetable oil conditioner. Background Art

[0002] Soil animals mainly move in complex soil ecosystems and play an important role in improving the physical and chemical properties of soil. In agricultural ecosystems, soil animals are closely related to agricultural farming systems and management methods. Agricultural farming or soil treatment changes the physical and chemical properties of soil, and also changes the living environment of soil animals, which in turn leads to changes in the species, behavior, number, and physiological conditions of soil animals. Due to the mutual influence between soil and soil animals, various changes in soil animals will also bring about changes in the physical and chemical properties of soil.

[0003] At present, there are many kinds of animals in soil animals. The more common dominant groups of large soil animals are Coleoptera, Gastropoda, Hymenoptera, and Oligochaeta, which account for more than half of the large soil animal species, while Lepidoptera, Diplopoda, Arachnids, and Isopoda account for about 10%. In addition, other dominant groups include Nematoda and Acarina, which account for more than 90% of small and medium-sized soil animals. These soil animals live in the soil for a long time or most of their lives. They change the physical and chemical properties of the soil, such as ventilation conditions, nutrient cycles, and microbial activities, through life activities such as feeding, excretion, and digging, and play a key role in changing the properties of the soil. For example, the activities of common Hymenoptera insects such as ants and Oligochaeta insects such as earthworms will significantly affect the physical and chemical properties of the soil, soil organic matter, and changes in soil nutrient cycles. In addition to environmental factors such as soil conditions and climate, changes in animal activities are also caused by the addition of exogenous substances. The most common exogenous substances, such as the application of pesticides, will significantly change the physiological activity and behavior of soil organisms, leading to the realization of insecticide purposes. However, the application of pesticides will also lead to a significant reduction in the number and types of animals in the soil. Long-term application is not conducive to the natural regulation between the soil and soil animals, which in turn causes changes in the physical and chemical properties of the soil. In severe cases, it will cause problems such as a decrease in soil fertility and weakened production capacity.

[0004] Tobacco bacterial wilt is a bacterial soil-borne disease caused by Ralstonia solanacearum. A method of using vegetable oil to control tobacco bacterial wilt has been developed. However, in-depth research in subsequent experiments found that long-term application of vegetable oil to the soil to prevent and control bacterial wilt will cause soil compaction and poor soil permeability. Therefore, it is urgent to find a new control method that uses vegetable oil to control bacterial wilt but does not cause soil compaction. Summary of the invention

[0005] In view of this, the purpose of the present invention is to provide a method for preventing and controlling tobacco bacterial wilt and soil compaction using vegetable oil and a vegetable oil conditioner thereof, by adding a vegetable oil conditioner to prevent tobacco bacterial wilt and at the same time using the vegetable oil conditioner to change the activity of soil animals, so as to solve the problem of soil compaction and poor air permeability caused by the long-term use of vegetable oil to prevent and control tobacco bacterial wilt.

[0006] The present invention solves the above technical problems by the following technical means:

[0007] A vegetable oil conditioner comprises the following raw materials: vegetable oil, eggshell powder, sodium citrate, whey protein, wormwood oil, peanut shell powder, L-methionine and riboflavin.

[0008] Furthermore, the raw materials include the following parts by weight: 10-15 parts of vegetable oil, 10-15 parts of eggshell powder, 2-5 parts of sodium citrate, 8-10 parts of whey protein, 0.5-0.8 parts of neem oil, 25-35 parts of peanut shell powder, 2-6 parts of L-methionine, and 0.5-1 parts of riboflavin.

[0009] Furthermore, the total amount of the vegetable oil conditioner used is 0.05-0.1 L / nest.

[0010] Furthermore, the vegetable oil is one or more of corn oil, sunflower oil, peanut oil and rapeseed oil.

[0011] Further, the preparation method of the vegetable oil conditioner is as follows:

[0012] The whey protein, L-methionine, riboflavin and water are mixed evenly, and then dried and sieved eggshell powder and peanut powder are added, and stirred evenly. Then, vegetable oil, schizonepeta oil and sodium citrate are added, and then ultrasonic homogenization is performed to obtain a vegetable oil conditioner.

[0013] Experimental verification shows that vegetable oil can significantly reduce the incidence of tobacco bacterial wilt, and also has a preventive and therapeutic effect on already infected tobacco. However, the effect of use still has room for improvement. Therefore, the present invention has re-prepared a vegetable oil conditioner, which can significantly improve the preventive and therapeutic effect while solving the problems of soil compaction and poor air permeability caused by multiple applications of vegetable oil to prevent and treat bacterial wilt.

[0014] Vegetable oil conditioners add effective nutrients. Vegetable oil is used to prevent and treat bacterial wilt. Peanut shell powder and eggshell powder are added. They can not only be used as fertilizers, but also have a certain adsorption capacity and can store other ingredients in vegetable oil conditioners. The more important role of peanut shell powder and eggshell powder is to act as good natural insect attractants and food to attract soil animals to improve the physical and chemical properties of the soil around tobacco seedlings, such as improving soil porosity and air permeability. The vitality, activity and feeding behavior of soil animals are related to the activity of common enzymes in animals, such as acetylcholinesterase, superoxide dismutase, and catalase. For example, the activity of acetylcholinesterase affects the activity of animal synaptic transmission, which in turn affects the sensitivity of nerve impulse conduction. Activating acetylcholinesterase can promote animal insects to maintain high vitality and activity, but while stimulating animal insects, it is necessary to prevent the acute phase of the enzyme from being significantly inhibited to prevent irreversible damage or death to the animal insects. Therefore, the vegetable oil conditioner of the present invention is also added with other raw materials. When insects move or feed near the rhizomes of tobacco after root irrigation, the oil substances cause enzyme inhibition on the animals and insects. The addition of sodium citrate, whey protein, L-methionine, and riboflavin raw materials can prevent or reduce the acute inhibition of the schizonepeta oil or vegetable oil on the animals and insects, significantly reduce the killing activity on the animals and insects, and also positively stimulate and activate the enzyme activity in the body, improve its vitality or activity and other physiological activities, promote its improvement effect on the soil in the area, and then improve soil compaction, improve soil permeability and organic matter content, so as to achieve the purpose of controlling bacterial wilt without causing too much impact on soil properties.

[0015] Furthermore, after the eggshell powder and peanut powder are added, the water content is 20-30%.

[0016] Further, the method for using the vegetable oil conditioner comprises the following steps:

[0017] (1) Before transplanting tobacco seedlings, apply vegetable oil conditioner to the nest, stir the soil in the nest evenly, and expose it to the sun for 3-4 days;

[0018] (2) 30 days after transplanting the tobacco seedlings, apply vegetable oil conditioner around the tobacco seedlings again and maintain them normally;

[0019] (3) 50 days after transplanting the tobacco seedlings, apply vegetable oil conditioner around the tobacco seedlings again and maintain them normally.

[0020] Although vegetable oil fungicides solve the problem of soil compaction and poor air permeability caused by the long-term use of vegetable oil to prevent and control tobacco bacterial wilt by changing the activity of animals in the soil, it is also necessary to pay attention to strictly control the ratio of vegetable oil fungicide raw materials, as well as the application amount and application interval, to prevent animal poisoning due to excessive accumulation of oil substances.

[0021] Furthermore, the mass ratio of the application amount of the vegetable oil conditioner in step (1), step (2) and step (3) is 1:(0.5-1):(0.2-0.4).

[0022] However, the total amount of the vegetable oil conditioner used per litter is preferably 0.05-0.1 L / litre.

[0023] Furthermore, in the step (2), 30 days after the tobacco seedlings are transplanted, the temperature when the vegetable oil conditioner is applied is ≤30°C, and the optimum temperature is 25-28°C.

[0024] Furthermore, in step (3), 50 days after the tobacco seedlings are transplanted, the temperature when the vegetable oil conditioner is applied is ≤35°C, and the optimal temperature is 25-30°C.

[0025] When applying vegetable oil conditioner to transplanted tobacco seedlings, it is important to keep the vegetable oil conditioner 5-10 cm away from the main rhizome.

[0026] Beneficial effects:

[0027] The conditioner prepared by the present invention not only has a control effect on tobacco bacterial wilt, but also has a long control time. At the same time, the added vegetable oil conditioner changes the activity of soil animals, thereby solving the problem of soil compaction and poor air permeability caused by long-term use of vegetable oil for controlling tobacco bacterial wilt. That is, by affecting the activity of soil animals, the physical and chemical properties of the soil such as pH, organic matter, water content, soil bulk density and porosity are significantly improved, so that the soil is loose and porous, suitable for the growth of tobacco, and the economic benefit is improved. At the same time, the abuse of pesticides is avoided, green planting is realized, and new ideas are provided for the research and development of green pesticides. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 :The effect of different treatments on tobacco growth and development;

[0029] Figure 2 : Effects of different treatments on tobacco root growth. DETAILED DESCRIPTION

[0030] The present invention will be described in detail below with reference to specific embodiments and accompanying drawings:

[0031] According to the data in Table 1, weigh the raw materials to prepare the vegetable oil conditioner.

[0032] Table 1 (unit: g)

[0033]

[0034] Example 1: Preparation of vegetable oil-killing conditioner

[0035] The preparation steps of the experimental group are as follows:

[0036] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have passed through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 10 minutes to obtain a vegetable oil conditioner.

[0037] Comparison Group 1:

[0038] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have passed through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, wormwood oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 8 minutes to obtain a vegetable oil conditioner.

[0039] Comparison Group 2:

[0040] Add whey protein, L-methionine and riboflavin into 30 ml of water, stir evenly, then add vegetable oil, wormwood oil and sodium citrate, and homogenize in a 1000 W homogenizer for 10 minutes by ultrasonication to obtain a vegetable oil sterilizing conditioner.

[0041] Comparison group 3:

[0042] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have passed through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil and wormwood oil are added and ultrasonically homogenized in a 1000W homogenizer for 10 minutes to obtain a vegetable oil conditioner.

[0043] Comparison group 4:

[0044] L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil, and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 10 minutes to obtain a vegetable oil conditioner.

[0045] Comparison group 5:

[0046] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 9 minutes to obtain a vegetable oil conditioner.

[0047] Comparison group 6:

[0048] Whey protein and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil, and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 10 minutes to obtain a vegetable oil conditioner.

[0049] Comparison group 7:

[0050] Whey protein and L-methionine are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil, and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 10 minutes to obtain a vegetable oil conditioner.

[0051] Comparison Group 8:

[0052] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 11 minutes to obtain a vegetable oil conditioner.

[0053] Comparison group 9:

[0054] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 9 minutes to obtain a vegetable oil conditioner.

[0055] Comparison group 10:

[0056] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 15 minutes to obtain a vegetable oil conditioner.

[0057] Comparison group 11:

[0058] Whey protein, L-methionine and riboflavin are added to a certain amount of water, and after mixing evenly, dried eggshell powder and peanut powder that have been sieved through a 100-mesh sieve are added, and then an appropriate amount of water is added to stir the whole mixture evenly. At this time, the water content of the mixture is about 25%. Then, vegetable oil, wormwood oil and sodium citrate are added and ultrasonically homogenized in a 1000W homogenizer for 5 minutes to obtain a vegetable oil conditioner.

[0059] Example 2: Method of using vegetable oil conditioner

[0060] (1) About one week before transplanting tobacco seedlings, apply vegetable oil fungicide to the nest, stir the soil in the nest evenly, and expose it to the sun for 3-4 days;

[0061] (2) 30 days after the tobacco seedlings are transplanted, when the temperature is ≤30°C, and the optimal temperature is 25-28°C, apply the vegetable oil conditioner again to the soil around the tobacco seedlings. When applying the vegetable oil conditioner, keep it 5-10 cm away from the main rhizome and maintain normal management;

[0062] (3) 50 days after transplanting tobacco seedlings, when the temperature is ≤35℃, and the optimal temperature is 25-30℃, apply vegetable oil conditioner around the tobacco seedlings again. When applying the vegetable oil conditioner, keep it 5-10cm away from the main rhizome and maintain normal management.

[0063] The total amount of vegetable oil conditioner used in each nest is 0.05-0.1L / nest, preferably 0.08L / nest, and the mass ratio of the vegetable oil conditioner applied three times is 1:(0.5-1):(0.2-0.4), among which the mass ratio of 1:0.8:0.3 is the best.

[0064] 1. Field control experiment of tobacco bacterial wilt

[0065] 1. Experimental location: The control experiment was conducted in Qingmuchi Village, Baima Town, Heshun Town, Wulong District, Chongqing. Tobacco has been planted in this area for many years, and bacterial wilt often occurs. The disease situation in the whole area is roughly the same. The terrain is flat. The soil conditions are: pH 4.83, organic matter 8.65, total nitrogen 1.80g / kg, total phosphorus 1.16g / kg, total potassium 12.08g / kg, alkaline nitrogen 121.99mg / kg, available phosphorus 96.66mg / kg, and available potassium 125mg / kg. Water content 0.51, soil bulk density 1.02, porosity 38.54.

[0066] 2. Experimental materials: Tobacco variety is Yunyan 87, transplanted in late April. The agents used are the plant oil fungicides of Example 1, Comparative Group 1 to Comparative Group 11, the control is 20% thiophanate-methyl, rapeseed oil, and clear water is used as a blank control.

[0067] 3. Experimental design: The total dosage of Example 1 and comparison groups 1-11 was 0.08 L / nest, 20% thiophanate-methyl was diluted 600 times, the total dosage was 0.3 L / nest, the total dosage of rapeseed oil was 0.02 L / nest, and the total dosage of clean water was 0.2 L / nest.

[0068] Example 1 and Comparative Examples 1-11, rapeseed oil and clean water were applied according to the method of Example 2, for a total of three times, the first time before transplanting, the second time 30 days after transplanting, and the third time 50 days after transplanting, and the ratio of each application per nest was 1:0.8:0.3.

[0069] Thiophanate-copper was applied for the first time when the roots were established after transplanting, and for the second time on the 15th day after transplanting. The dosage for each application was 0.25L / nest.

[0070] The experiment was set up with 3 replicates, arranged in random blocks, and each plot had an area of ​​20m 2 , 30 tobacco plants, and protection rows are set up around the plot. No other pesticides are used to prevent and control corresponding diseases.

[0071] 4. Indicators and methods

[0072] The number of diseased plants in each plot was investigated and recorded at the peak of the disease. The disease grade was implemented according to GB / T23222-2008 standard, and the disease index, incidence rate and control effect were calculated respectively. The first incidence rate survey was conducted on August 10, and the second survey was conducted on August 20.

[0073] Incidence rate (%) = number of diseased plants / total number of plants investigated × 100;

[0074] Disease index = Σ(disease level × number of plants with that disease level) / (highest disease level × total number of plants investigated) × 100;

[0075] Relative control effect (%) = [(disease index of clean water control area - disease index of treated area) / disease index of clean water control area] × 100.

[0076] 5. Results and Analysis

[0077] The results obtained from the above tests are shown in Table 2.

[0078] Table 2

[0079]

[0080]

[0081] Analyzing the data in Table 2, we can see that:

[0082] 1. Comparing Example 1 with the clear water control group, the incidence rate in the first survey was reduced by 33.34%, the disease index was reduced by 22.86, and the relative prevention effect was 85.72. In the second survey, the incidence rate was reduced by 36.67%, the disease index was reduced by 27.14, and the relative prevention effect was 80.28, indicating that the conditioning agent disclosed in the present invention has good prevention and control effects, and the prevention and control effects are high in both surveys, and can be used for long-term prevention and control of tobacco bacterial wilt.

[0083] 2. Compared with the control effect of rapeseed oil or thiophanate-methyl in Example 1, the incidence rate and disease index in the first and second surveys were reduced, and the relative control effect was high, indicating that the vegetable oil in Example 1 has a synergistic effect in the control of bacterial wilt after being compounded with other disclosed raw materials.

[0084] 3. Compared with control groups 1 to 7, the incidence rates of Example 1 in the first and second surveys were significantly reduced, the disease index decreased significantly, and the relative prevention effect was good, indicating that the raw material components and proportions of the conditioning agent disclosed in the present invention are better, and can prevent and treat tobacco bacterial wilt for a longer period of time and maintain a good control effect.

[0085] 4. Compared with control groups 8 and 9, Example 1 differs only in the change in the dosage of wormwood oil. Compared with control groups 10 and 11, the only difference is the different ratios of wormwood oil and vegetable oil. The incidence rate and disease index of the two surveys of control groups 8-11 were increased, and the relative control effect was reduced, indicating that excessive wormwood oil or improper ratio can easily lead to a decrease in the control effect.

[0086] 2. Tobacco Growth and Development Regulation Experiment

[0087] During the experiment, the growth and root growth of tobacco were investigated 40 days after the last application. Tobacco plants were randomly selected in each plot to test the plant height, leaf area, root length, and root dry weight of tobacco. Three replicates were performed. The results are shown in Table 3. Figure 1and Figure 2 shown.

[0088] Table 3

[0089] deal with Plant height / cm <![CDATA[Leaf area / cm 2 > Root length / cm Root dry weight / g Example 1 102.35 1123.41 24.23 324.54 Comparison group 1 98.34 1023.23 20.75 286.25 Comparison group 2 97.54 986.54 21.25 275.34 Comparison group 3 96.23 1012.43 19.24 286.53 Comparison group 4 95.64 985.67 20.54 275.65 Comparison group 5 93.45 967.42 19.86 275.34 Comparison group 6 98.76 1012.34 19.21 265.87 Comparison group 7 94.54 956.87 18.67 286.53 Comparison group 8 96.34 954.68 20.54 278.91 Comparison group 9 93.32 987.25 19.45 284.32 Comparison group 10 95.65 968.36 20.56 275.65 Comparison group 11 96.54 994.36 19.67 267.61 Rapeseed Oil 94.52 953.23 20.34 281.23 Shimizu 88.34 925，65 18.43 265.41

[0090] According to Table 2 and Figure 1 , Figure 2 The results show that the growth of tobacco treated with Example 1 is better than that of other treatments, wherein the plant height, leaf area, root length and root dry weight are better than those of the water treatment, especially the plant height and root dry weight reach 102.35 cm and 324.54 g, wherein the number of lateral roots in the root system and the root length have obvious advantages, and each growth of Example 1 is significantly better than that of other comparative examples, indicating that the conditioning agent prepared by the present invention not only does not affect the growth of tobacco plants, but significantly promotes the growth and development of tobacco, which is beneficial to increasing income and production.

[0091] 3. Soil physical and chemical properties experiment

[0092] During the experiment, the initial values ​​of soil physical and chemical properties in the nest were tested once before the experiment, the second time was on the 60th day after transplanting, and the third time was 20 days after tobacco harvest.

[0093] The initial values ​​are: pH 4.81, organic matter 8.25, water content 0.50, soil bulk density 1.03, and porosity 35.85.

[0094] The results are shown in Table 4:

[0095] Table 4

[0096]

[0097]

[0098] 4. Soil Animal Activity Test

[0099] 1. Experimental animals: earthworms (Eisenia fetida), over 2 months old, weighing about 400-600 mg.

[0100] 2. Experimental materials: In the experiment, soil grown in Wulong tobacco area all year round (pH 4.85, organic matter 8.63, total nitrogen 1.81 g / kg, total phosphorus 1.16 g / kg, total potassium 12.02 g / kg, alkaline nitrogen 121.43 mg / kg, available phosphorus 94.24 mg / kg, available potassium 124.21 mg / kg, water content 0.53, soil bulk density 1.09, porosity 35.45) was used. Example 1, comparative groups 1-11 and rapeseed oil were added as additives to the artificial soil respectively. The addition amount of each group was artificial soil: additive = 500 g: 0.08 L, and the mixture was fully stirred. Clear water was used as a blank control. Ten healthy earthworms were added to each group, and three replicates were performed.

[0101] 3. Experimental conditions: Place healthy earthworms that have been cleansed for 24 hours into each group of experimental boxes and seal them with gauze to prevent the earthworms from escaping. Place the earthworms in an artificial climate box at a temperature of 25±1℃, a humidity of 80%±2%, an illumination intensity of 800lx, and a light-dark ratio of 14h:10h. After all the earthworms have entered the soil, add 2.0g of dry cow dung to the soil surface as food for the earthworms. Supplement soil moisture every 7 days for a period of 20 days of cultivation. In addition, investigate the weight of the earthworms, the number of deaths, and the difference in enzyme activity in the earthworms (CAT, POD enzymes). The data are shown in Table 5:

[0102] Table 5

[0103] deal with Weight loss rate / % mortality rate / % <![CDATA[CAT / U·mg -1 ]]> <![CDATA[POD / U·mg -1 ]]> Example 1 8.42 5.56 7.85 1.51 Comparison group 1 10.24 7.78 8.69 1.62 Comparison group 2 10.58 11.11 8.97 1.65 Comparison group 3 10.87 12.22 8.95 1.62 Comparison group 4 10.81 10.00 9.25 1.64 Comparison group 5 11.25 8.89 9.36 1.63 Comparison group 6 12.65 11.11 9.17 1.65 Comparison group 7 12.25 12.22 9.22 1.61 Comparison group 8 10.85 7.78 9.24 1.64 Comparison group 9 10.68 10.00 9.16 1.63 Comparison group 10 9.65 11.11 8.96 1.62 Comparison group 11 10.24 11.11 8.85 1.64 Rapeseed Oil 14.24 14.44 10.58 1.65 Shimizu 18.65 20.00 12.02 1.71

[0104] According to the results of Table 4 and Table 5, the following conclusions are obtained:

[0105] 1. Compared with the initial values, the pH of Example 1 increased by 0.11 and 0.04, the organic matter content increased by 0.73 and 0.72, the water content increased by 0.17 and 0.15, the soil bulk density increased by 0.15, and the porosity increased by 10.73 and 9.73, respectively, in the first survey and the second survey. This shows that the conditioning agent prepared in Example 1 can significantly improve the physical and chemical conditions of the soil when applied to the soil, and the effect can be maintained for a long time. According to the results of Table 4, when the conditioning agent of Example 1 is mixed with soil to feed earthworms, the weight loss rate is small and the mortality rate is low, especially the difference in the enzyme activity of CAT and POD is the smallest, which shows that while maintaining the high activity of earthworms, the acute phase of enzyme production is prevented from being significantly inhibited, thereby reducing its mortality rate, thereby promoting its improvement of the physical and chemical properties of the soil.

[0106] 2. Rapeseed oil or clean water cannot provide enough nutrition for earthworms, resulting in a significant decrease in their weight loss rate. Excessive clean water will cause the soil moisture content to be too high, which can easily lead to a high mortality rate and a high difference in enzyme activity changes in earthworms. The addition of rapeseed oil also led to an increase in the mortality rate and the difference in enzyme activity changes in earthworms. The reason is that no raw materials such as sodium citrate, whey protein, and L-methionine were added, which increased the acute inhibition of earthworms caused by oil substances, thereby increasing the killing activity of earthworms, resulting in excessively high mortality rates and excessive differences in enzyme activity changes.

[0107] 3. Combining the data in Table 4 and Table 5, it can be seen from the comparison between Example 1 and comparison groups 1-11 that the earthworms in Example 1 have higher activity, lower mortality, and significantly improve the physical and chemical conditions of the soil, indicating that while adding vegetable oil conditioners to prevent and control tobacco bacterial wilt, vegetable oil conditioners can also be used to change the activity of soil animals, so as to solve the problem of soil compaction and poor air permeability caused by the long-term use of vegetable oil to prevent and control tobacco bacterial wilt.

[0108] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the purpose and scope of the technical solutions of the present invention, which should be included in the scope of the claims of the present invention. The techniques, shapes, and structural parts not described in detail in the present invention are all known technologies.

Claims

1. A vegetable oil conditioning agent, characterized in that The vegetable oil conditioning agent comprises the following raw materials: Vegetable oil, eggshell powder, sodium citrate, whey protein, wormwood oil, peanut shell powder, L-methionine and riboflavin. The raw materials include the following parts by weight: 10-15 parts of vegetable oil, 10-15 parts of eggshell powder, 2-5 parts of sodium citrate, 8-10 parts of whey protein, 0.5-0.8 parts of wormwood oil, 25-35 parts of peanut shell powder, 2-6 parts of L-methionine and 0.5-1 parts of riboflavin.

2. A vegetable oil conditioner according to claim 1, characterized in that: The total amount of the vegetable oil conditioner used is 0.05-0.1 L / nest.

3. A vegetable oil conditioning agent according to claim 2, characterized in that: The vegetable oil is one or more of corn oil, sunflower oil, peanut oil and rapeseed oil.

4. A vegetable oil conditioning agent according to claim 3, characterized in that: The preparation method of the vegetable oil conditioner is as follows: The whey protein, L-methionine, riboflavin and water are mixed evenly, and then dried and sieved eggshell powder and peanut powder are added, and stirred evenly. Then, vegetable oil, schizonepeta oil and sodium citrate are added, and then ultrasonic homogenization is performed to obtain a vegetable oil conditioner.

5. A vegetable oil conditioning agent according to claim 4, characterized in that: After the eggshell powder and peanut powder are added, the water content is 20-30%.

6. The method for preventing bacterial wilt and soil compaction by using vegetable oil according to claim 5, characterized in that: The method comprises the following steps: (1) Before transplanting tobacco seedlings, apply vegetable oil conditioner to the nest, stir the soil in the nest evenly, and expose it to the sun for 3-4 days; (2) 30 days after transplanting the tobacco seedlings, apply vegetable oil conditioner around the tobacco seedlings again; (3) 50 days after transplanting the tobacco seedlings, apply vegetable oil conditioner around the tobacco seedlings again.

7. The method for preventing bacterial wilt and soil compaction by using vegetable oil according to claim 6, characterized in that: The mass ratio of the application amount of the vegetable oil conditioner in step (1), step (2) and step (3) is 1: (0.5-1): (0.2-0.4).

8. The method for preventing bacterial wilt and soil compaction by using vegetable oil according to claim 7, characterized in that: In the step (2), 30 days after the tobacco seedlings are transplanted, the temperature when the vegetable oil conditioner is applied is ≤30°C.

9. The method for preventing bacterial wilt and soil compaction by using vegetable oil according to claim 8, characterized in that: In the step (3), 50 days after the tobacco seedlings are transplanted, the temperature when the vegetable oil conditioner is applied is ≤35°C.

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