Apparatus and method for stimulated Raman detection

Abstract

According to the technical scheme, the device and method are characterized in that the device comprises a femtosecond stimulated Raman spectrometer body, supporting frames are fixedly installed on the two sides of the femtosecond stimulated Raman spectrometer body respectively, a side plate is arranged on one side of each supporting frame, a baffle plate is arranged on one side of each side plate, and the femtosecond stimulated Raman spectrometer body is fixedly installed on the other side of each supporting frame. The femtosecond stimulated Raman spectrometer comprises a femtosecond stimulated Raman spectrometer body, a supporting frame, a side plate, a shielding plate, a transparent plate, an operation column, a temperature rising frame, a heating wire, a blocking net, a metal net, a supporting frame and a micro fan which are matched for use; the femtosecond stimulated Raman spectrometer prevents the high-humidity environment from condensing water vapor on the surface of an optical element of the femtosecond stimulated Raman spectrometer body to influence light path transmission and signal intensity, and prevents the moisture content in a sample from being increased due to the increase of humidity, so that the molecular structure and the vibration mode of the sample are changed to influence generation and transmission of Raman signals.

Description

Technical Field

[0001] The present invention relates to the technical field of stimulated Raman detection, and in particular to a device and a method for stimulated Raman detection. Background Art

[0002] Stimulated Raman scattering refers to the strong interaction between high-intensity lasers and material molecules, which makes the scattering process have the properties of stimulated emission. This scattered light is Raman scattered light, so this nonlinear optical effect is called stimulated Raman scattering. Stimulated Raman scattering exhibits threshold characteristics, just like a laser, it can only be produced with appropriate pump power. Stimulated Raman scattering microscopy technology uses this principle to achieve rapid scanning imaging of biological samples without fluorescent labeling. This technology has the advantages of high sensitivity, non-destructiveness, and high spatial resolution. It has been widely used in many fields such as cell biology, tumor research, and neuroscience research. The equipment for stimulated Raman detection mainly includes femtosecond stimulated Raman spectrometers, collinear multi-beam stimulated Raman scattering microscopes, and phase-locked amplifiers. Femtosecond stimulated Raman spectrometers are a type of nonlinear optical spectroscopy with the advantages of time-resolved vibrational spectroscopy, allowing additional tools for interpreting and assigning molecular dynamics and detecting "dark" molecular states.

[0003] In the prior art, femtosecond stimulated Raman spectrometer is an advanced time-resolved vibrational spectroscopy technology used to study the structural dynamics of molecular excited states. However, during the detection of samples, factors such as temperature, humidity and vibration may have a certain impact on its performance. When using femtosecond stimulated Raman spectrometer in humid weather such as in the south, the high humidity environment may cause condensation of water vapor on the surface of the optical components, affecting the optical path transmission and signal intensity. At the same time, humidity changes may change the physical and chemical properties of the sample, especially for samples that are hygroscopic or easily affected by moisture, their Raman spectra may be significantly affected, which may affect the detection accuracy of the femtosecond stimulated Raman spectrometer. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a device and method for stimulated Raman detection to solve the problems raised in the above background technology.

[0005] The above technical objectives of the present invention are achieved through the following technical solutions:

[0006] The device and method for stimulated Raman detection include a femtosecond stimulated Raman spectrometer body, support frames are fixedly installed on both sides of the femtosecond stimulated Raman spectrometer body, a side panel is provided on one side of the support frame, a shielding plate is provided on one side of the side panel, observation holes are respectively opened on one side of the side panel and the shielding plate, and a transparent plate is fixedly sleeved inside the observation hole; a temperature and humidity adjustment component is arranged on one side of the two side panels, and the temperature and humidity adjustment component includes: two storage holes, the two storage holes are respectively opened on one side of the two side panels, an operating column is arranged inside the storage hole, a heating rack is fixedly installed on one side of the operating column, a heating wire is fixedly installed inside the heating rack, a blocking net is fixedly sleeved inside the heating rack, a metal net is fixedly installed on one side of the heating rack, a support frame is fixedly installed on the bottom surface of the operating column, a mounting hole is opened on one side of the support frame, and a fixing hole is fixed inside the mounting hole. A micro fan is sleeved, an air outlet slot is provided on one side of the operating column, a dustproof net is fixedly sleeved inside the air outlet slot, a cooling hole is provided on one side of the operating column, the cooling hole is communicated with the air outlet slot, a semiconductor cooling sheet is fixedly installed inside the cooling hole, a heat dissipation net is fixedly sleeved inside the cooling hole, and the hot end of the semiconductor cooling sheet faces the heat dissipation net, an exhaust hole is provided on one side of the operating column, the exhaust hole is communicated with the air outlet slot, an exhaust fan is fixedly installed inside the exhaust hole, a PLC controller is fixedly installed on one side of the femtosecond stimulated Raman spectrometer body, and the PLC controller is electrically connected to the heating wire, the micro fan, the semiconductor cooling sheet and the exhaust fan respectively; a detection component for detecting the temperature and humidity of the femtosecond stimulated Raman spectrometer body is provided on one side of the operating column; an adjustment component for moving the operating column is provided inside the storage hole.

[0007] By adopting the above technical solution, through the setting of the heating wire, firstly, the position of the operating column and the heating rack is moved to the sample detection position of the femtosecond stimulated Raman spectrometer body by using the adjustment component, when the position of the operating column and the heating rack is close to the sample stage of the femtosecond stimulated Raman spectrometer body, by using the heating wire, the temperature of the heating wire is increased and the temperature inside the heating rack is increased, and then the micro fan is started at the same time, after the micro fan generates wind force, the wind force blown by the micro fan will reach the position of the heating wire through the metal mesh, and then the hot wind will be discharged through the blocking mesh and reach the sample detection position of the femtosecond stimulated Raman spectrometer body, so as to reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body and prevent the high humidity environment from causing the femtosecond stimulated Raman spectrometer body to be blown away. Water vapor condenses on the surface of the optical elements of the femtosecond stimulated Raman spectrometer, affecting the light path transmission and signal strength, while preventing the increase in humidity from causing an increase in the moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and further affecting the generation and transmission of the Raman signal. Then, by using a semiconductor refrigeration sheet, the cooling surface of the semiconductor refrigeration sheet faces the inside of the air outlet slot, and then after refrigeration, the cold air of the semiconductor refrigeration sheet is extracted by using an exhaust fan and sprayed out through a dustproof net, thereby facilitating the reduction of the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body, preventing the temperature at the detection position from being too high, leading to intensified molecular motion in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and linearity of the Raman signal.

[0008] Preferably, the detection component includes: a detection platform, the detection platform is fixedly installed on one side of the operating column, a fixing groove is opened on one side of the detection platform, a temperature sensor is fixedly sleeved on the inner circular wall surface of the fixing groove, and the temperature sensor is electrically connected to the PLC controller; a mounting groove is opened on one side of the detection platform, a humidity sensor is fixedly sleeved on the inner circular wall surface of the mounting groove, and the humidity sensor is electrically connected to the PLC controller.

[0009] By adopting the above technical solution, through the set temperature sensor, before using the femtosecond stimulated Raman spectrometer body to detect the sample, during the preparation work, the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body can be detected through the temperature sensor. When the temperature is too high, the temperature sensor will transmit a signal to the PLC controller, and then the PLC controller will control the semiconductor refrigeration plate and the exhaust fan to start, so as to reduce the temperature around the detection position of the femtosecond stimulated Raman spectrometer body. At the same time, the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body can be detected through the humidity sensor. When the humidity is too high, the humidity sensor will transmit a signal to the PLC controller, and then the PLC controller will start the heating wire and the micro fan, so as to timely reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body.

[0010] Preferably, the adjustment assembly includes: an adjustment column, the adjustment column is movably sleeved inside the receiving hole, a limiting groove is opened on one side of the adjustment column, an electric push rod is fixedly sleeved inside the limiting groove, the telescopic rod of the electric push rod is fixedly installed with the operating column, the top and bottom surfaces of the adjustment column are respectively fixedly installed with a rotating column, the inner top and bottom surfaces of the receiving hole are respectively opened with a rotating groove and a rotating hole, the rotating column located at the top of the adjustment column is movably sleeved with the rotating hole, the rotating column located at the bottom of the adjustment column is movably sleeved with the rotating groove, a stepper motor is fixedly installed on the top surface of the side panel, and the driving shaft of the stepper motor is fixedly installed with the rotating column.

[0011] By adopting the above technical solution, by setting up an adjustment column, by using a stepper motor, the rotation of the driving shaft of the stepper motor will drive the rotating column, the adjustment column, the operating column and the heating rack to rotate, so that the operating column and the heating rack can be moved out of the storage hole, and then by using an electric push rod, the movement of the telescopic rod of the electric push rod will push the operating column and the heating rack to move, so that the operating column, the heating rack and the detection table can be close to the sample detection position of the femtosecond stimulated Raman spectrometer body, thereby facilitating the detection of the humidity and temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body, so that the sample can be detected in a suitable environment.

[0012] Preferably, fixed blocks are fixedly installed on the top surface and one side of the side panel, the positions of the two fixed blocks correspond to each other, a rotating column is fixedly installed on the side where the two fixed blocks are close to each other, and sliding grooves are respectively opened on the top surface and bottom surface of the shielding plate, and the rotating column is movably connected to the sliding groove.

[0013] By adopting the above technical solution, through the setting of the shielding plate, when the environmental humidity and temperature have a greater impact on sample detection, first, the initial positions of the side plate and the shielding plate are parallel, and then the shielding plate is slid to allow the rotating column to reach the inner end of the sliding groove, and then the shielding plate is rotated to make the side plate and the shielding plate form an L shape. At this time, the two shielding plates will block the front of the femtosecond stimulated Raman spectrometer body, thereby facilitating the shielding of the sample detected by the femtosecond stimulated Raman spectrometer body.

[0014] Preferably, a support hole is opened on one side of the shielding plate, a spring clip frame is fixedly installed inside the support hole, a limiting frame is fixedly installed on one side of the side plate, a clip column is fixedly installed inside the limiting frame, and the clip column is movably clipped to the spring clip frame.

[0015] By adopting the above technical solution, through the provided snap-in column, when the shielding plate is stored and parallel to the position of the side plate, by pressing one end of the shielding plate, the snap-in column is snapped into the interior of the spring snap-in frame, thereby facilitating the restriction of the shielding plate.

[0016] Preferably, a guide column is fixedly installed on one side of the interior of the support frame, a blocking block is provided on one side of the support frame, two clipping plates are fixedly installed on one side of the blocking block, two clipping grooves are provided on one side of the support frame, the clipping plates are movably connected with the clipping grooves, a support column is fixedly installed on one side of the side plate, a positioning hole is provided on one side of the support column, and the positioning hole is movably connected with the guide column.

[0017] By adopting the above technical solution, a blocking block is set up, and the clamping plate is moved by moving the blocking block to move the blocking block away from one side of the support frame. Subsequently, the support column is moved by moving the side panel, and the support column can be pulled out from the inside of the support frame, thereby facilitating the disassembly of the side panel.

[0018] Preferably, a dehumidification box is provided on the top surface of the support frame, a partition net is fixedly installed inside the dehumidification box, a sealing groove is provided on the inner wall of the dehumidification box, a filter net is movably sleeved inside the dehumidification box, a snap-on frame is fixedly sleeved outside the filter net, and when the snap-on frame is movably engaged with the sealing groove, two snap-on slots are provided on the top surface of the support frame, and two snap-on blocks are fixedly installed on the bottom surface of the dehumidification box, and the snap-on blocks are movably engaged with the snap-on slots.

[0019] By adopting the above technical solution, by setting up a dehumidification box and by placing a dehumidifier inside the dehumidification box, the humidity of the detection environment of the femtosecond stimulated Raman spectrometer body can be reduced in severely humid weather in the south.

[0020] Preferably, a drainage hole is provided on one side of the dehumidification box, the inner circumferential wall of the drainage hole is provided with threads, the inner circumferential wall of the drainage hole is threadedly connected to a sealing plug, and the outer circumferential wall of the sealing plug is provided with threads.

[0021] By adopting the above technical solution, the humidity and moisture absorbed inside the dehumidification box can be discharged.

[0022] The present invention also provides a method for stimulated Raman detection, the specific steps of which are as follows:

[0023] S1: By using a stepper motor to drive the rotating column, the adjusting column, the operating column and the heating rack to rotate, the operating column and the heating rack can be moved out of the storage hole, and then by using an electric push rod, the movement of the telescopic rod of the electric push rod will push the operating column and the heating rack to move, so that the first step can be achieved to make the operating column, the heating rack and the detection table close to the sample detection position of the femtosecond stimulated Raman spectrometer body;

[0024] S2: Before using the femtosecond stimulated Raman spectrometer to detect the sample, during the preparation work, the temperature of the detection environment of the femtosecond stimulated Raman spectrometer can be detected by the temperature sensor. When the temperature is too high, the temperature sensor will transmit the signal to the PLC controller, and then the PLC controller will control the semiconductor cooling plate and the exhaust fan to start, so as to achieve the second step to detect the temperature around the sample detection position of the femtosecond stimulated Raman spectrometer;

[0025] S 3: By using an exhaust fan to extract the cold air from the semiconductor refrigeration chip and spray it out through the dustproof net, the third step can be achieved to reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body, to prevent the temperature at the detection position from being too high, which will lead to the intensification of molecular motion in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and line shape of the Raman signal;

[0026] S 4: The humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body can be detected by the humidity sensor. When the humidity is too high, the humidity sensor transmits a signal to the PLC controller, and the PLC controller starts the heating wire and the micro fan, so as to reach the fourth step to detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body;

[0027] S 5: By increasing the temperature of the heating wire, the micro fan is started at the same time. The wind blown by the micro fan will reach the position of the heating wire through the metal mesh, and then the hot air will be discharged through the blocking mesh and reach the sample detection position of the femtosecond stimulated Raman spectrometer body. The fifth step can be achieved to reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body to prevent the increase in humidity from causing an increase in the moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and further affecting the generation and transmission of the Raman signal.

[0028] In summary, the present invention mainly has the following beneficial effects:

[0029] The femtosecond stimulated Raman spectrometer body, support frame, side panels, shielding plates, observation holes, transparent plates, operating columns, heating racks, heating wires, blocking nets, metal nets, support racks and micro fans are used in coordination with each other, so that hot air is discharged through the blocking nets and reaches the sample detection position of the femtosecond stimulated Raman spectrometer body, thereby facilitating the reduction of humidity around the detection position of the femtosecond stimulated Raman spectrometer body, preventing high humidity environment from causing condensation of water vapor on the surface of the optical element of the femtosecond stimulated Raman spectrometer body, affecting light path transmission and signal strength, and preventing an increase in humidity from causing an increase in moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and further affecting the generation and transmission of Raman signals.

[0030] By using the operating column, air outlet slot, dustproof net, cooling hole, semiconductor cooling sheet, heat dissipation net, exhaust hole and exhaust fan in coordination with each other, it is convenient to reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body, and prevent the temperature at the detection position from being too high, which will lead to the intensification of molecular movement in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and line shape of the Raman signal. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a three-dimensional structural schematic diagram of the present invention;

[0032] Figure 2 It is a schematic diagram of the side plate structure of the present invention;

[0033] Figure 3 It is a schematic diagram of the shielding plate structure of the present invention;

[0034] Figure 4 It is a schematic diagram of the support frame structure of the present invention;

[0035] Figure 5 It is a schematic diagram of the transparent plate structure of the present invention;

[0036] Figure 6 It is a schematic diagram of the structure of the spring clip frame of the present invention;

[0037] Figure 7 It is a schematic diagram of the support column structure of the present invention;

[0038] Figure 8 It is a schematic diagram of the structure of the operating column of the present invention;

[0039] Fig. 9 It is a schematic diagram of the structure of the regulating column of the present invention;

[0040] Fig.10 It is a schematic diagram of the structure of the dehumidification box of the present invention.

[0041] Figure numerals: 1. femtosecond stimulated Raman spectrometer body; 2. support frame; 3. side panel; 4. shielding plate; 5. observation hole; 6. transparent plate; 7. storage hole; 8. operating column; 9. heating rack; 10. heating wire; 11. blocking net; 12. metal net; 13. support frame; 14. mounting hole; 15. micro fan; 16. air outlet slot; 17. dustproof net; 18. cooling hole; 19. semiconductor cooling sheet; 20. heat dissipation net; 21. exhaust hole; 22. exhaust fan; 23. test table; 24. fixing slot; 25. temperature sensor; 26. mounting slot; 27. humidity sensor; 28. Adjusting column; 29, limiting groove; 30, electric push rod; 31, rotating column; 32, rotating groove; 33, rotating hole; 34, stepping motor; 35, fixing block; 36, rotating column; 37, sliding groove; 38, supporting hole; 39, spring clamping frame; 40, limiting frame; 41, clamping column; 42, guide column; 43, blocking block; 44, clamping plate; 45, clamping groove; 46, supporting column; 47, positioning hole; 48, dehumidification box; 49, partition net; 50, sealing groove; 51, filter net; 52, clamping frame; 53, drainage hole; 54, sealing plug; 55, clamping groove; 56, clamping block. DETAILED DESCRIPTION

[0042] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0043] Example 1: Reference Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Fig. 9, a device and method for stimulated Raman detection, comprising a femtosecond stimulated Raman spectrometer body 1, wherein support frames 2 are fixedly installed on both sides of the femtosecond stimulated Raman spectrometer body 1, a side plate 3 is arranged on one side of the support frame 2, a shielding plate 4 is arranged on one side of the side plate 3, observation holes 5 are respectively opened on one side of the side plate 3 and the shielding plate 4, a transparent plate 6 is fixedly sleeved inside the observation hole 5, a temperature and humidity adjustment component is arranged on one side of the two side plates 3, the temperature and humidity adjustment component comprises two receiving holes 7, the two receiving holes 7 are respectively opened on one side of the two side plates 3, an operating column 8 is arranged inside the receiving hole 7, a heating frame 9 is fixedly installed on one side of the operating column 8, a heating wire 10 is fixedly installed inside the heating frame 9, and a blocking net 11 is fixedly sleeved inside the heating frame 9 A metal mesh 12 is fixedly installed on one side of the heating frame 9, a support frame 13 is fixedly installed on the bottom surface of the operating column 8, a mounting hole 14 is opened on one side of the inner side of the support frame 13, a micro fan 15 is fixedly sleeved inside the mounting hole 14, an air outlet slot 16 is opened on one side of the operating column 8, a dustproof net 17 is fixedly sleeved inside the air outlet slot 16, a cooling hole 18 is opened on one side of the operating column 8, the cooling hole 18 is connected to the air outlet slot 16, a semiconductor cooling sheet 19 is fixedly installed inside the cooling hole 18, a heat dissipation net 20 is fixedly sleeved inside the cooling hole 18, and the hot end of the semiconductor cooling sheet 19 faces the heat dissipation net 20, an exhaust hole 21 is opened on one side of the operating column 8, the exhaust hole 21 is connected to the air outlet slot 16, and an exhaust fan is fixedly installed inside the exhaust hole 21 22. A PLC controller is fixedly installed on one side of the femtosecond stimulated Raman spectrometer body 1. The PLC controller is electrically connected to the heating wire 10, the micro fan 15, the semiconductor cooling sheet 19 and the exhaust fan 22 respectively. A detection component for detecting the temperature and humidity of the femtosecond stimulated Raman spectrometer body 1 is arranged on one side of the operating column 8. An adjusting component for moving the operating column 8 is arranged inside the storage hole 7. Through the arranged heating wire 10, the operating column 8 and the heating rack 9 are first moved to the sample detection position of the femtosecond stimulated Raman spectrometer body 1 by using the adjusting component. When the operating column 8 and the heating rack 9 are close to the sample stage of the femtosecond stimulated Raman spectrometer body 1, the temperature of the heating wire 10 is increased by using the heating wire 10. The temperature inside the heating rack 9 is increased, and then the micro fan 15 is started at the same time. After the micro fan 15 generates wind, the wind blown by the micro fan 15 will pass through the metal mesh 12 to the position of the heating wire 10, and then the hot air will be discharged through the blocking mesh 11 and reach the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so as to reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1, prevent the high humidity environment from causing condensation of water vapor on the surface of the optical element of the femtosecond stimulated Raman spectrometer body 1, affecting the light path transmission and signal strength, and prevent the increase in humidity from causing the increase in moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and then affecting the generation and transmission of the Raman signal, and then, by using the semiconductor refrigeration sheet 19,The cooling surface of the semiconductor cooling sheet 19 faces the inside of the air outlet slot 16. After cooling, the cold air of the semiconductor cooling sheet 19 is extracted by using the exhaust fan 22 and sprayed out through the dustproof net 17, so as to reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body 1, and prevent the temperature of the detection position from being too high, which will lead to the intensification of the molecular motion in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and line shape of the Raman signal.

[0044] Embodiment 2: Based on the above embodiment 1, reference Figure 1 , Figure 2 , Figure 3 , Figure 5 , Figure 6 , Figure 7 , Figure 8 and Fig. 9The detection component includes a detection platform 23, which is fixedly installed on one side of the operating column 8. A fixing groove 24 is provided on one side of the detection platform 23, and a temperature sensor 25 is fixedly sleeved on the inner wall of the fixing groove 24. The temperature sensor 25 is electrically connected to the PLC controller. A mounting groove 26 is provided on one side of the detection platform 23, and a humidity sensor 27 is fixedly sleeved on the inner wall of the mounting groove 26. The humidity sensor 27 is electrically connected to the PLC controller. By setting the temperature sensor 25, before using the femtosecond stimulated Raman spectrometer body 1 to detect the sample, during the preparation work, the temperature sensor 25 can be used to detect the humidity in the environment of the femtosecond stimulated Raman spectrometer body 1. The temperature is detected. When the temperature is too high, the temperature sensor 25 will transmit a signal to the PLC controller, and then the PLC controller will control the semiconductor refrigeration plate 19 and the exhaust fan 22 to start, so as to reduce the temperature around the detection position of the femtosecond stimulated Raman spectrometer body 1. At the same time, the humidity sensor 27 can detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1. When the humidity is too high, the humidity sensor 27 will transmit a signal to the PLC controller, and then the PLC controller will start the heating wire 10 and the micro fan 15, so as to timely reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1, and adjust the components. The side plate 3 includes an adjusting column 28, which is movably sleeved in the interior of the storage hole 7. A limiting groove 29 is provided on one side of the adjusting column 28. An electric push rod 30 is fixedly sleeved in the interior of the limiting groove 29. The telescopic rod of the electric push rod 30 is fixedly installed with the operating column 8. A rotating column 31 is fixedly installed on the top and bottom surfaces of the adjusting column 28 respectively. A rotating groove 32 and a rotating hole 33 are provided on the top and bottom surfaces of the storage hole 7 respectively. The rotating column 31 at the top of the adjusting column 28 is movably sleeved in the rotating hole 33. The rotating column 31 at the bottom of the adjusting column 28 is movably sleeved in the rotating groove 32. A stepping motor 34 is fixedly installed on the top surface of the side plate 3. The driving shaft of the stepping motor 34 is fixedly mounted on the rotating column 31. The fixed installation is carried out by setting the adjustment column 28. By using the stepper motor 34, the rotation of the driving shaft of the stepper motor 34 will drive the rotating column 31, the adjustment column 28, the operating column 8 and the heating rack 9 to rotate, so that the operating column 8 and the heating rack 9 can be moved out of the storage hole 7. Then, by using the electric push rod 30, the movement of the telescopic rod of the electric push rod 30 will push the operating column 8 and the heating rack 9 to move, so that the operating column 8, the heating rack 9 and the detection table 23 can be close to the sample detection position of the femtosecond stimulated Raman spectrometer body 1, thereby facilitating the detection of the humidity and temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so that the sample can be detected in a suitable environment.

[0045] Embodiment 3: Based on the above embodiment 1 or 2, refer to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 and Figure 7 The top surface and one side of the side plate 3 are respectively fixedly installed with fixed blocks 35, and the positions of the two fixed blocks 35 correspond to each other. A rotating column 36 is fixedly installed on the side where the two fixed blocks 35 are close to each other. The top surface and the bottom surface of the shielding plate 4 are respectively provided with sliding grooves 37, and the rotating column 36 is movably connected with the sliding grooves 37. Through the setting of the shielding plate 4, when the environmental humidity and temperature have a greater impact on the sample detection, first, the initial positions of the side plate 3 and the shielding plate 4 are parallel, and then the shielding plate 4 is slid to allow the rotating column 36 to The shielding plate 4 reaches one end of the inner part of the sliding groove 37, and then rotates the shielding plate 4 to make the side plate 3 and the shielding plate 4 form an L shape. At this time, the two shielding plates 4 will block the front of the femtosecond stimulated Raman spectrometer body 1, so as to facilitate shielding the sample detected by the femtosecond stimulated Raman spectrometer body 1. A support hole 38 is opened on one side of the shielding plate 4, and a spring clamping frame 39 is fixedly installed inside the support hole 38. A limiting frame 40 is fixedly installed on one side of the side plate 3, and a clamping column 41 is fixedly installed inside the limiting frame 40. The clamping column 4 1 is movably connected with the spring clamping frame 39, and through the provided clamping column 41, when the shielding plate 4 is stored and parallel to the position of the side plate 3, by pressing one end of the shielding plate 4, the clamping column 41 is clamped into the inside of the spring clamping frame 39, so as to facilitate the restriction of the shielding plate 4, a guide column 42 is fixedly installed on one side of the inner side of the support frame 2, a blocking block 43 is provided on one side of the support frame 2, two clamping plates 44 are fixedly installed on one side of the blocking block 43, and two clamping grooves 45 are provided on one side of the support frame 2. 44 is movably engaged with the engaging groove 45, a support column 46 is fixedly installed on one side of the side panel 3, a positioning hole 47 is opened on one side of the support column 46, and the positioning hole 47 is movably sleeved with the guide column 42, and a blocking block 43 is set, and the engaging plate 44 is moved by moving the blocking block 43, and the blocking block 43 is removed from one side of the support frame 2. Subsequently, the support column 46 is moved by moving the side panel 3, and the support column 46 can be pulled out from the inside of the support frame 2, thereby facilitating the disassembly of the side panel 3.

[0046] Embodiment 4: Based on the above embodiment 1, 2 or 3, reference Figure 1 , Figure 2 , Figure 4 and Fig.10A dehumidification box 48 is provided on the top surface of the support frame 2, and a partition net 49 is fixedly installed inside the dehumidification box 48. A sealing groove 50 is provided on the inner wall of the dehumidification box 48. A filter net 51 is movably sleeved inside the dehumidification box 48, and a clamping frame 52 is fixedly sleeved outside the filter net 51. When the clamping frame 52 is movably clamped with the sealing groove 50, two clamping grooves 55 are provided on the top surface of the support frame 2, and two clamping blocks 56 are fixedly installed on the bottom surface of the dehumidification box 48. The clamping blocks 56 are movably clamped with the clamping grooves 55. By setting the dehumidification box 48, by putting a dehumidifier inside the dehumidification box 48, the humidity of the detection environment of the femtosecond stimulated Raman spectrometer body 1 can be reduced in severely humid weather in the south. A drainage hole 53 is provided on one side of the dehumidification box 48, and the inner circular wall surface of the drainage hole 53 is provided with a thread. The inner circular wall surface of the drainage hole 53 is threadedly connected with a sealing plug 54, and the outer circular wall surface of the sealing plug 54 is provided with a thread.

[0047] Embodiment 5: Based on the above embodiment 1, 2, 3 or 4, refer to Figure 1-Figure 10 The present invention also provides a method for stimulated Raman detection, the specific steps are as follows:

[0048] S1: By using the stepping motor 34 to drive the rotating column 31, the adjusting column 28, the operating column 8 and the heating rack 9 to rotate, the operating column 8 and the heating rack 9 can be moved out of the storage hole 7, and then by using the electric push rod 30, the movement of the telescopic rod of the electric push rod 30 will push the operating column 8 and the heating rack 9 to move, so as to achieve the first step of making the operating column 8, the heating rack 9 and the detection table 23 close to the sample detection position of the femtosecond stimulated Raman spectrometer body 1;

[0049] S2: Before using the femtosecond stimulated Raman spectrometer body 1 to detect the sample, during the preparation work, the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body 1 can be detected by the temperature sensor 25. When the temperature is too high, the temperature sensor 25 will transmit a signal to the PLC controller, and then the PLC controller will control the semiconductor cooling sheet 19 and the exhaust fan 22 to start, so as to achieve the second step to detect the temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body 1;

[0050] S 3: By using the exhaust fan 22 to extract the cold air from the semiconductor refrigeration chip 19 and spraying it through the dustproof net 17, the third step can be achieved to reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body 1, to prevent the temperature at the detection position from being too high, which will lead to the intensification of the molecular motion in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and line shape of the Raman signal;

[0051] S 4: The humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1 can be detected by the humidity sensor 27. When the humidity is too high, the humidity sensor 27 transmits a signal to the PLC controller, and the PLC controller starts the heating wire 10 and the micro fan 15, so as to reach the fourth step to detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1;

[0052] S 5: By increasing the temperature of the heating wire 10, the micro fan 15 is started at the same time. The wind blown by the micro fan 15 will reach the position of the heating wire 10 through the metal mesh 12, and then the hot wind will be discharged through the blocking mesh 11 and reach the sample detection position of the femtosecond stimulated Raman spectrometer body 1. The fifth step can be achieved to reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1 to prevent the increase in humidity from causing an increase in the moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and further affecting the generation and transmission of the Raman signal.

[0053] Working principle: Please refer to Figure 1-Figure 10 As shown, by setting the heating wire 10, firstly, the position of the operating column 8 and the heating rack 9 is moved to the sample detection position of the femtosecond stimulated Raman spectrometer body 1 by using the adjustment component. When the position of the operating column 8 and the heating rack 9 is close to the sample stage of the femtosecond stimulated Raman spectrometer body 1, by using the heating wire 10, the temperature of the heating wire 10 increases and the temperature inside the heating rack 9 increases at the same time, and then the micro fan 15 is started at the same time. After the micro fan 15 generates wind force, the wind force blown by the micro fan 15 will pass through the metal mesh 12 to reach the position of the heating wire 10, and then the hot air will be discharged through the blocking mesh 11 and reach the sample detection position of the femtosecond stimulated Raman spectrometer body 1, thereby facilitating the reduction of the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1 to prevent a high humidity environment. This causes water vapor to condense on the surface of the optical element of the femtosecond stimulated Raman spectrometer body 1, affecting the light path transmission and signal strength, while preventing the increase in humidity from causing an increase in the moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and further affecting the generation and transmission of the Raman signal. Then, by using a semiconductor refrigeration sheet 19, the cooling surface of the semiconductor refrigeration sheet 19 faces the inside of the air outlet slot 16, and then after refrigeration, the cold air of the semiconductor refrigeration sheet 19 is extracted by using an exhaust fan 22 and sprayed out through the dustproof net 17, thereby facilitating the reduction of the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body 1, preventing the temperature of the detection position from being too high, resulting in intensified molecular motion in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and linearity of the Raman signal.

[0054] By setting the temperature sensor 25, before using the femtosecond stimulated Raman spectrometer body 1 to detect the sample, during the preparation work, the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body 1 can be detected by the temperature sensor 25. When the temperature is too high, the temperature sensor 25 will transmit a signal to the PLC controller, and then the PLC controller will control the semiconductor refrigeration plate 19 and the exhaust fan 22 to start, so as to reduce the temperature around the detection position of the femtosecond stimulated Raman spectrometer body 1. At the same time, the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body 1 can be detected by the humidity sensor 27. When the humidity is too high, the humidity sensor 27 will transmit a signal to the PLC controller, and then the PLC controller will start the heating wire 10 and the micro fan 15, so as to timely reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body 1.

[0055] By setting the adjustment column 28, by using the stepper motor 34, the rotation of the driving shaft of the stepper motor 34 will drive the rotating column 31, the adjustment column 28, the operating column 8 and the heating rack 9 to rotate, so that the operating column 8 and the heating rack 9 can be moved out of the storage hole 7, and then by using the electric push rod 30, the movement of the telescopic rod of the electric push rod 30 will push the operating column 8 and the heating rack 9 to move, so that the operating column 8, the heating rack 9 and the detection table 23 can be close to the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so as to facilitate the detection of the humidity and temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body 1, so that the sample can be detected in a suitable environment.

[0056] By setting the shielding plate 4, when the environmental humidity and temperature have a greater impact on sample detection, first, the initial positions of the side plate 3 and the shielding plate 4 are parallel, and then the shielding plate 4 is slid to allow the rotating column 36 to reach the inner end of the sliding groove 37, and then the shielding plate 4 is rotated to make the side plate 3 and the shielding plate 4 form an L shape. At this time, the two shielding plates 4 will block the front of the femtosecond stimulated Raman spectrometer body 1, so as to facilitate shielding the sample detected by the femtosecond stimulated Raman spectrometer body 1.

[0057] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for stimulated Raman detection, characterized in that include: A femtosecond stimulated Raman spectrometer body (1), wherein support frames (2) are fixedly mounted on both sides of the femtosecond stimulated Raman spectrometer body (1), a side plate (3) is arranged on one side of the support frame (2), a shielding plate (4) is arranged on one side of the side plate (3), observation holes (5) are respectively opened on one side of the side plate (3) and the shielding plate (4), and a transparent plate (6) is fixedly sleeved inside the observation hole (5); A temperature and humidity adjustment component is arranged on one side of the two side panels (3), and the temperature and humidity adjustment component comprises: two storage holes (7), the two storage holes (7) are respectively opened on one side of the two side panels (3), an operating column (8) is arranged inside the storage hole (7), a heating frame (9) is fixedly installed on one side of the operating column (8), a heating wire (10) is fixedly installed inside the heating frame (9), a blocking net (11) is fixedly sleeved inside the heating frame (9), a metal net (12) is fixedly installed on one side of the heating frame (9), a support frame (13) is fixedly installed on the bottom surface of the operating column (8), a mounting hole (14) is opened on one side of the support frame (13), a micro fan (15) is fixedly sleeved inside the mounting hole (14), an air outlet slot (16) is opened on one side of the operating column (8), and the air outlet slot (16) is fixedly sleeved inside the air outlet slot (16). A dustproof net (17) is fixedly sleeved, a cooling hole (18) is provided on one side of the operating column (8), the cooling hole (18) is connected to the air outlet slot (16), a semiconductor cooling sheet (19) is fixedly installed inside the cooling hole (18), a heat dissipation net (20) is fixedly sleeved inside the cooling hole (18), the hot end of the semiconductor cooling sheet (19) faces the heat dissipation net (20), an exhaust hole (21) is provided on one side of the operating column (8), the exhaust hole (21) is connected to the air outlet slot (16), an exhaust fan (22) is fixedly installed inside the exhaust hole (21), and a PLC controller is fixedly installed on one side of the femtosecond stimulated Raman spectrometer body (1), and the PLC controller is electrically connected to the heating wire (10), the micro fan (15), the semiconductor cooling sheet (19) and the exhaust fan (22) respectively; A detection component for detecting the temperature and humidity of the femtosecond stimulated Raman spectrometer body (1) is provided on one side of the operating column (8); An adjustment component for moving the operating column (8) is arranged inside the receiving hole (7).

2. The device for stimulated Raman detection according to claim 1, characterized in that: The detection component comprises: A detection platform (23), the detection platform (23) is fixedly mounted on one side of the operating column (8), a fixing groove (24) is provided on one side of the detection platform (23), a temperature sensor (25) is fixedly sleeved on the inner circular wall surface of the fixing groove (24), and the temperature sensor (25) is electrically connected to the PLC controller; a mounting groove (26) is provided on one side of the detection platform (23), a humidity sensor (27) is fixedly sleeved on the inner circular wall surface of the mounting groove (26), and the humidity sensor (27) is electrically connected to the PLC controller.

3. The device for stimulated Raman detection according to claim 1, characterized in that: The adjustment component comprises: An adjusting column (28), wherein the adjusting column (28) is movably sleeved inside the receiving hole (7), a limiting groove (29) is provided on one side of the adjusting column (28), an electric push rod (30) is fixedly sleeved inside the limiting groove (29), a telescopic rod of the electric push rod (30) is fixedly mounted on the operating column (8), a rotating column (31) is fixedly mounted on the top and bottom surfaces of the adjusting column (28), a rotating groove (32) and a rotating hole (33) are respectively provided on the top and bottom surfaces of the receiving hole (7), the rotating column (31) located at the top of the adjusting column (28) is movably sleeved with the rotating hole (33), and the rotating column (31) located at the bottom of the adjusting column (28) is movably sleeved with the rotating groove (32), a stepping motor (34) is fixedly mounted on the top surface of the side plate (3), and a driving shaft of the stepping motor (34) is fixedly mounted on the rotating column (31).

4. The device for stimulated Raman detection according to claim 1, characterized in that: A fixing block (35) is fixedly mounted on the top surface and one side of the side plate (3), the positions of the two fixing blocks (35) correspond to each other, a rotating column (36) is fixedly mounted on the side where the two fixing blocks (35) are close to each other, and a sliding groove (37) is respectively provided on the top surface and the bottom surface of the shielding plate (4), and the rotating column (36) is movably sleeved with the sliding groove (37).

5. The device for stimulated Raman detection according to claim 1, characterized in that: A support hole (38) is provided on one side of the shielding plate (4), a spring clip frame (39) is fixedly installed inside the support hole (38), a limiting frame (40) is fixedly installed on one side of the side plate (3), a clip column (41) is fixedly installed inside the limiting frame (40), and the clip column (41) is movably clipped with the spring clip frame (39).

6. The device for stimulated Raman detection according to claim 1, characterized in that: A guide column (42) is fixedly mounted on one side of the inner part of the support frame (2); a blocking block (43) is provided on one side of the support frame (2); two clamping plates (44) are fixedly mounted on one side of the blocking block (43); two clamping grooves (45) are provided on one side of the support frame (2); the clamping plates (44) are movably clamped with the clamping grooves (45); a support column (46) is fixedly mounted on one side of the side plate (3); a positioning hole (47) is provided on one side of the support column (46); the positioning hole (47) is movably sleeved with the guide column (42).

7. The device for stimulated Raman detection according to claim 1, characterized in that: A dehumidification box (48) is arranged on the top surface of the support frame (2), a partition net (49) is fixedly installed inside the dehumidification box (48), a sealing groove (50) is provided on the inner wall of the dehumidification box (48), a filter net (51) is movably sleeved inside the dehumidification box (48), a snap-on frame (52) is fixedly sleeved outside the filter net (51), and when the snap-on frame (52) is movably snap-fitted with the sealing groove (50), two snap-on grooves (55) are provided on the top surface of the support frame (2), and two snap-on blocks (56) are fixedly installed on the bottom surface of the dehumidification box (48), and the snap-on blocks (56) are movably snap-fitted with the snap-on grooves (55).

8. The device for stimulated Raman detection according to claim 7, characterized in that: A drainage hole (53) is provided on one side of the dehumidification box (48), the inner circumferential wall surface of the drainage hole (53) is provided with a thread, the inner circumferential wall surface of the drainage hole (53) is threadedly connected to a sealing plug (54), and the outer circumferential wall surface of the sealing plug (54) is provided with a thread.

9. A method for stimulated Raman detection, characterized in that The following steps are involved: S1: By using a stepper motor (34) to drive the rotating column (31), the adjusting column (28), the operating column (8) and the heating rack (9) to rotate, the operating column (8) and the heating rack (9) can be moved out of the storage hole (7), and then by using an electric push rod (30), the movement of the telescopic rod of the electric push rod (30) will push the operating column (8) and the heating rack (9) to move, so as to achieve the first step of making the operating column (8), the heating rack (9) and the detection platform (23) close to the sample detection position of the femtosecond stimulated Raman spectrometer body (1); S2: Before using the femtosecond stimulated Raman spectrometer body (1) to detect a sample, during the preparation work, the temperature in the detection environment of the femtosecond stimulated Raman spectrometer body (1) can be detected by the temperature sensor (25). When the temperature is too high, the temperature sensor (25) will transmit a signal to the PLC controller, and then the PLC controller will control the semiconductor cooling plate (19) and the exhaust fan (22) to start, so as to achieve the second step of detecting the temperature around the sample detection position of the femtosecond stimulated Raman spectrometer body (1); S3: By using the exhaust fan (22) to extract the cold air from the semiconductor cooling plate (19) and spraying it out through the dustproof net (17), the third step can be achieved to reduce the temperature of the sample detection position of the femtosecond stimulated Raman spectrometer body (1), so as to prevent the temperature at the detection position from being too high, which will lead to the intensification of the molecular motion in the sample, thereby increasing the frequency of inelastic scattering events, and further affecting the intensity and line shape of the Raman signal; S 4: The humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body (1) can be detected by the humidity sensor (27). When the humidity is too high, the humidity sensor (27) transmits a signal to the PLC controller, and the PLC controller starts the heating wire (10) and the micro fan (15), so as to reach the fourth step to detect the humidity around the sample detection position of the femtosecond stimulated Raman spectrometer body (1); S 5: By increasing the temperature of the heating wire (10), the micro fan (15) is started at the same time. The wind blown by the micro fan (15) will reach the position of the heating wire (10) through the metal mesh (12), and then the hot wind will be discharged through the blocking mesh (11) and reach the sample detection position of the femtosecond stimulated Raman spectrometer body (1). The fifth step can be achieved to reduce the humidity around the detection position of the femtosecond stimulated Raman spectrometer body (1) to prevent the increase in humidity from causing an increase in the moisture content in the sample, thereby changing the molecular structure and vibration mode of the sample, and further affecting the generation and transmission of the Raman signal.

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