Detection kit and detection method for H1, H3 and H9 type avian influenza viruses

A detection kit and avian influenza virus technology, which are applied in the field of biology, can solve the problems such as cumbersome detection of avian influenza virus, difficulty in meeting requirements, etc., and achieve the effects of quick and simple detection, simplified detection steps, and shortened detection time.

Active Publication Date: 2016-02-03
INST OF ANIMAL HUSBANDRY & VETERINARY SCI SHANXI ACAD OF AGRI SCI SAAS +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the detection of avian influenza virus in the prior art is cumbersome and dif...

Method used

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  • Detection kit and detection method for H1, H3 and H9 type avian influenza viruses
  • Detection kit and detection method for H1, H3 and H9 type avian influenza viruses
  • Detection kit and detection method for H1, H3 and H9 type avian influenza viruses

Examples

Experimental program
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Embodiment 1

[0059] Depend on figure 1 As shown, Example 1 of the present invention provides a H1, H3 and H9 type avian influenza virus detection kit, which may specifically include an RNA extraction kit, an RNA reverse transcription kit and an RPA detection kit. Wherein, the RNA extraction kit is mainly used to extract the RNA of the sample to be tested as a detection template, and the RNA extraction kit contains an RNA extraction reagent, and in the process of extracting the RNA of the sample to be tested, the RNA extraction reagent acts on The sample RNA to be tested is finally obtained as a detection template.

[0060] The RNA reverse transcription kit is mainly used to reverse transcribe the RNA in the detection template to obtain cDNA. Specifically, the RNA reverse transcription kit contains RNA reverse transcription reagents. When the RNA in the template is reverse-transcribed, the RNA reverse transcription reagent acts on the detection template RNA, and then cDNA is obtained by re...

Embodiment 2

[0078] On the basis of Example 1, the kit described in the embodiment of the present invention may further include a reagent strip for detecting the amplified DNA sequence, and the reagent strip is a rapid chromogenic reagent strip. Using the rapid color development reagent strip in the kit can quickly and easily detect the avian influenza virus without RPA amplification detector and other equipment conditions.

Embodiment 3

[0080] as attached figure 2 As shown, the embodiment of the present invention provides a kind of H1, H3 and H9 type avian influenza virus detection method, wherein, described specific method comprises:

[0081] S101, the extraction reagent extracts the RNA of the sample to be tested as a detection template;

[0082] Specifically, the RNA in the sample to be tested is extracted by an RNA extraction kit, and the RNA extraction kit includes an RNA extraction reagent, and the RNA extraction reagent acts on the sample to be tested to extract RNA, and then the extracted RNA as a detection template.

[0083] S102. Perform reverse transcription on the detection template to obtain cDNA;

[0084] Specifically, the RNA reverse transcription kit performs reverse transcription on the detection template, and the RNA reverse transcription kit has an RNA reverse transcription reagent, which acts on the detection template, performs reverse transcription, and finally obtains cDNA .

[0085...

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Abstract

The invention discloses a detection kit and detection method for H1, H3 and H9 type avian influenza viruses and belongs to the technical field of biology. The kit comprises an RNA extraction kit, an RNA reverse transcription kit and an RPA detection kit. The RNA extraction kit is used for extracting RNA of a to-be-detected sample to be used as a detection template. The RNA reverse transcription kit is used for conducting reverse transcription on the RNA in the detection template to obtain cDNA. The RPA detection kit comprises primers and fluorescent dye. The primers are used for amplifying the cDNA to obtain an amplified DNA sequence. The primers comprise the forward primer and the reverse primer. The fluorescent dye is used for conducting fluorescence detection on the amplified DNA sequence. According to the detection kit and the detection method, the avian influenza viruses can be rapidly, easily and conveniently detected.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a detection kit and detection method for H1, H3 and H9 type avian influenza viruses. Background technique [0002] Avian influenza (Avian influenza, AI) is an acute and severe infectious disease of poultry caused by type A influenza virus of the family Orthomyxoviridae. Classified as a class A infectious disease. Avian influenza virus (AIV) belongs to type A influenza virus. Avian influenza virus (AIV) mutates frequently and has many serotypes. According to the difference of HA antigenicity, it is divided into 16 subtypes of HA. Strong antigen variability, wide range of hosts, and no cross-protection between subtypes lead to frequent outbreaks of avian influenza, and due to the extreme incubation period of avian influenza, sudden outbreaks, and sudden death without any clinical symptoms, the morbidity and mortality can be as high as 100 %, becoming a devastating disease in...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/68
CPCC12Q1/686C12Q1/701C12Q2521/507C12Q2563/107C12Q2565/625
Inventor 李红丽伏显华王红宝郭雪丽孙爱娟
Owner INST OF ANIMAL HUSBANDRY & VETERINARY SCI SHANXI ACAD OF AGRI SCI SAAS
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