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Method for detecting surface enhanced Raman spectra of blood RNA

A surface-enhanced Raman and spectral detection technology, applied in the application field of biomedical optical technology in life sciences, can solve the research reports of SERS spectral detection and other issues

Inactive Publication Date: 2011-09-07
FUJIAN NORMAL UNIV
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Problems solved by technology

At present, there are reports on the detection of biological macromolecules such as DAN and RNA in tissues and cells, and better SERS spectra have been obtained, but there is no research report on the detection of SERS spectra of RNA in human blood. If this research is applied in practice , will benefit the detection of human colon cancer or other cancers

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  • Method for detecting surface enhanced Raman spectra of blood RNA
  • Method for detecting surface enhanced Raman spectra of blood RNA
  • Method for detecting surface enhanced Raman spectra of blood RNA

Examples

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Embodiment 1

[0029] 1. Separate the serum from the whole blood of patients with colon cancer and normal people, and store it in a -80°C refrigerator within two hours to prevent RNA cleavage. Use Trizol LS reagent to extract RNA from serum, and use 30 μL DEPC water Dissolve the RNA and store it in a -80°C refrigerator for later use. Use NanoDrop ND 1000 to quantify the RNA at a concentration of 18.8 to 56.4 ng / mL.

[0030]2. Dissolve 10 mg of silver nitrate in 50 ml of deionized water, heat to 100°C to make it boil, then add 1 ml of trisodium citrate with a concentration of 1% dropwise, and stir rapidly, after the dropwise addition Continue to keep boiling for 6 hours to obtain silver sol. After the silver sol is naturally cooled, use a centrifuge to centrifuge the above silver sol at 10,000 rpm for 10 minutes to make the silver sol layered. The supernatant is discarded, and the concentrated silver sol in the lower layer is taken.

[0031] 3. Take 5 μl of concentrated silver sol and drop it...

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Abstract

The invention relates to a preparation method of a silver nanometer membrane for detecting surface enhanced Raman spectra (SERS) of ribonucleic acid (RNA), and belongs to the technical field of medical science. In the method, the RNA in blood is extracted, and SERS spectra with strong signals and more spectrum peaks are acquired by taking the prepared silver nanometer membrane as an enhanced substrate to reflect the information of RNA molecules fully; and the enhanced substrate prepared by the method is contrasted to various enhanced substrates simultaneously to obtain the conclusion that the information acquired in the process of detecting the RNA molecules by using the enhanced substrate is the most and highest in strength, so high-quality SERS spectrograms can be acquired. Therefore, the high-quality spectrograms can be used for statistic analysis to obtain differences between patients and normal person so as to establish the foundation for the application of SERS detection to clinical diagnosis. The method has the advantages of short time for measurement and analysis and small using amount of samples, and is convenient and accurate in detection.

Description

technical field [0001] The invention relates to a method for detecting blood RNA surface-enhanced Raman spectroscopy, more specifically, extracting RNA molecules from human blood, dropping them on silver nano-films, and then detecting RNA molecules through surface-enhanced Raman spectroscopy The Raman (SERS) spectrum signal belongs to the application field of biomedical optical technology in life science. Background technique: [0002] An ideal early cancer screening test requires high sensitivity, high specificity, rapid and low-damage test results, in order to effectively prevent the continued development of cancer and reduce cancer mortality. But there is currently no method that matches this ideal screening technique. For example, commonly used methods for detecting colon cancer include sigmoidoscopy, barium enema, and colonoscopy. Although the sensitivity and specificity are relatively high, these inspections are invasive and costly. In contrast, the fecal occult bloo...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 陈燕坪陈荣冯尚源席刚琴林多陈希文林筱茜
Owner FUJIAN NORMAL UNIV
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