Porcine reproductive and respiratory syndrome virus RT-LAMP detection kit and detection method thereof

A technology of RT-LAMP and respiratory syndrome, applied in the field of veterinary biological products, can solve the problems of long cycle, cumbersome operation, unsuitable for antigen detection, etc.
CN101575650AActive Publication Date: 2009-11-11CHINA INST OF VETERINARY DRUG CONTROL
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA INST OF VETERINARY DRUG CONTROL
Publication Date
2009-11-11

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Abstract

The invention relates to a porcine reproductive and respiratory syndrome virus RT-LAMP detection kit and a detection method thereof. Primers required by PRSSV RT-LAMP reaction system are designed according to the sequence of porcine reproductive and respiratory syndrome virus (PRSSV) published by GenBank; PRSSV virus RNA is extracted with the virus RNA extraction reagent (LBBII-RNA) designed and prepared by the inventor, the PRSSV RT-LAMP reaction system established in the invention is utilized for detection, and color developing agent is added after the reaction to judge the result; the result shows that the PRSSV virus RNA obtains efficient specific amplification after the reaction is conducted for 45 minutes at the temperature of 63 DEG C; and then, quick detection of porcine reproductive and respiratory syndrome virus American classical strain and NSP2 variant strain (highly pathogenic porcine reproductive and respiratory syndrome virus strain) is conducted by SpuI enzyme cutting. Compared with the prior art, the invention has quick detection, high sensitivity, low reaction cost, convenient and fast operation, which is capable of differentiating American classical strain and NSP2 variant strain (highly pathogenic porcine reproductive and respiratory syndrome virus strain) and meets the requirement of multi-level detection.
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Description

technical field

[0001] The invention relates to a porcine reproductive and respiratory syndrome virus RT-LAMP detection kit and a detection method thereof, belonging to the disease diagnosis technology in the field of veterinary biological products. Background technique

[0002] Porcine Reproductive and Respiratory Syndrome, also known as PRRS, is an infectious disease characterized by reproductive disorders in sows and respiratory diseases in piglets caused by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). Infectious diseases that pose a greater threat to the pig industry.

[0003] PRRSV belongs to the nestoviridae and Arteriviridae. This enveloped virus is a positive-sense single-stranded RNA virus, and its gene length is 15kb. The current PRRSV virus strains prevalent in China are the American type and the NSP2 mutated American type PRRSV strain (highly pathogenic PRRSV strain). At present, the detection methods of the virus include RT-PCR method, cell se...

Claims

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