Pleuronectiformes soleidae fish ribosome internal transcribed spacer region 2 general primer and design and amplification method thereof

Abstract

The invention relates to a pleuronectiformes soleidae fish ribosome internal transcribed spacer region 2 general primer capable of being used for fast identifying pleuronectiformes soleidae fishes anda design and amplification method thereof. The provided pleuronectiformes soleidae fish ribosome internal transcribed spacer region 2 general primer can be used for efficiently and specially amplifying pleuronectiformes soleidae fish octopus ITS2; a plurality of species can be simultaneously amplified in one step; the advantages of high efficiency, high speed and convenience are realized; in addition, the length differences of ITS2 sequences in different species of the soleidae fishes are obvious; the direct naked eye observation can be performed through a gel electrophoresis technology; further, the species with the similar forms or damaged forms can be efficiently, fast and conveniently identified.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a general primer for transcriptional spacer 2 in ribosome ribosomes and a method for designing and amplifying them, which can be used for rapid identification of fishes of the family Soleidae. Background technique [0002] Soleidae, as the most abundant group of flatfishes, has about 35 genera and 175 species. Many species have very important economic value. The study of the transcriptional spacer 2 in the ribosome is even more blank. [0003] The internal transcribed spacer 1 (ITS2) is a non-coding region located between the 5.8S rDNA and 28S rDNA of the ribosomal RNA coding gene. The evolution rate of 5.8S rDNA and 28S rDNA is slow, and the sequence is very conservative, while the evolution rate of ITS2 is relatively fast, basically tends to be similar within the species, but shows significant differences between species, so it can be used as a tool to distinguish di...

AI Technical Summary

Problems solved by technology

[0004] On September 29, 2017, the Chinese Patent Office disclosed the invention authorization of a spider mitochondrial genome full-sequence amplification primer and amplification method, the authorization publication number: CN104531688B. Long PCR (L-PCR) amplification and sequencing can identify spiders, but it can only amplify spiders; it also uses DNA technology to identify organisms, but it cannot identify specific biological species, and must Only by sequencing can it be accurately determined, and it is necessary to design multiple pairs of primers to amplify the entire genome sequence, which takes a long time and is cumbersome.

Images