The invention discloses beta-mannase, a coding gene as well as a producing strain and application thereof. The fungus of the high-temperature beta-mannase is Aspergillus sp.T16 with a preservation number of CCTCC NO: M2013122. The novel high-temperature mannase gene manT16 obtained by cloning the fungus has a nucleotide sequence as shown in SEQ ID NO.2 or 3. The beta-mannase obtained by coding has an amino acid sequence as shown in SEQ ID NO.1, as well as a recombinant vector for coding the mannase gene and yeast genetically engineered bacterium. The mannase has an optimum temperature of 75 DEG C and an optimum pH value of 3.5-5.0, and is tolerable to the high temperature of 75 DEG C and stable under the pH value of 2.2-8.0. Moreover, as novel high temperature enzyme, the beta-mannase has a great production application value and extensive application prospect in industries of feed, food, medicines, wine brewing, energy and the like.