Genetically engineered Max Kluyveromyces yeast and its applications

By screening and modifying Kluyveromyces martensii, a genetically engineered strain with high cholesterol production was constructed, solving the problems of heavy pollution, high cost, and safety in the production of ergosterol and cholesterol in existing technologies, and achieving efficient and stable cholesterol production.

CN121801726BActive Publication Date: 2026-05-26ENZYMECODE BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202610297445.3
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2026-03-12
Publication Date
2026-05-26
Estimated Expiration
2046-03-12

AI Technical Summary

Technical Problem

Existing technologies for the production of ergosterol and cholesterol suffer from heavy pollution, high costs, fluctuating raw material supply, and safety issues. Furthermore, there are no reports of large-scale cholesterol production using genetically engineered brewing yeast, and the application of Kluyveromyces martensii in this area has not been reported at all.

Method used

By screening for high ergosterol-producing Kluyveromyces martensii, haploid strains were isolated and modified to construct high cholesterol-producing genetically engineered strains. Through genetic engineering, key enzyme genes were overexpressed in Kluyveromyces martensii to optimize metabolic pathways and improve cholesterol production levels.

Benefits of technology

It achieved efficient and stable cholesterol synthesis with a yield of 7.64 g/L, setting a new record for the highest cholesterol production by microorganisms and providing a green and safe production method.

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Abstract

This invention discloses a genetically engineered strain of *Kluyveromyces martensii* and its applications, belonging to the field of bioengineering technology. The invention discloses a genetically engineered *Kluyveromyces martensii* strain, firstly obtained through screening a strain that produces high levels of ergosterol; based on this, it induces and isolates... Eye A haploid strain was constructed and then metabolically optimized through the introduction of heterologous DHCR24 and DWF5 genes, weakening of the competitive pathway gene ERG6, increasing the metabolic flux of the post-squalene pathway during sterol synthesis, peroxisome compartmentalization engineering of the mevalonate pathway, knockout of the hypoxia-inhibiting transcription factor ROX1, improved cofactor supply, and optimization of the yeast lipid pathway, resulting in a high-cholesterol-producing engineered strain. Through fed-batch fermentation, efficient and stable cholesterol synthesis was achieved, with a yield of 7.64 g / L, laying the foundation for cholesterol biomanufacturing.
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