Cutting propagation method for splash-ink dendrobe old stems

A technology of cutting propagation and old stems, which is applied in the field of asexual propagation of Dendrobium nobile, can solve the problems of ornamental value and economic value decline, high technical content, virus accumulation, etc., and achieve high bud induction rate and survival rate, high genetic stability, The effect of extending the service life

Active Publication Date: 2016-11-16
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Pomo" Dendrobium aseptic sowing requires certain aseptic culture conditions, the operation is complicated, and the separation of offspring is serious, and it is difficult to obtain offspring with neat traits.
It takes a long time for tissue culture to establish a tissue culture rapid propagation technology system, and the technical content is high, which is difficult for flower growers to master, which is not conducive to technology promotion
Branch propagation can easily lead to the accumulation and spread of viruses, resulting in the reduction of ornamental quality
At the same time, aseptic sowing and tissue culture of "Pomo" Dendrobium can easily cause offspring to return to their ancestors and obtain white-flowered offspring, which significantly reduces the ornamental value and economic value.

Method used

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  • Cutting propagation method for splash-ink dendrobe old stems

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Collection of old stems

[0022] In August 2014, 20 2-year-old, bare, leafless, and slightly shrunken old stems were selected and cut with scissors as cuttings. The length of the stem segment was 5-8 cm.

[0023] The collection time of old stems should be selected after the temperature warms up in spring or in high temperature weather in summer and autumn. In winter, the temperature is low and it is difficult to induce new buds.

[0024] stem segment processing

[0025] Cut the incision end of the lower end of the old stem flat, save 0.5 cm from the stem at the lower end incision, and the length of the stem segment is 5-8 cm, and the incision is coated with 75% carbendazim powder.

[0026] stem cuttings

[0027] The three substrates (diameter: 2-3cm) of bark, stone and charcoal are mixed at a ratio of 2:1:1 as the cutting substrate, placed in a culture tray, and the treated old stems are placed flat on the surface of the substrate.

[0028] Management after inser...

Embodiment 2

[0033] Collection of old stems

[0034] In July 2015, 20 3-year-old to perennial, bare, leafless, and slightly shrunken middle and upper old stems were selected and cut with scissors as cuttings. The length of the stem segment was 5-8 cm.

[0035] stem segment processing

[0036] Cut the incision end of the lower end of the old stem flat, save 0.5 cm from the stem at the lower end incision, and the length of the stem segment is 5-8 cm, and the incision is coated with 75% carbendazim powder.

[0037] stem cuttings

[0038] The three substrates (diameter: 2-3cm) of bark, stone and charcoal are mixed at a ratio of 2:1:1 as the cutting substrate, placed in a culture tray, and the treated old stems are placed flat on the surface of the substrate.

[0039] Management after insertion

[0040] Watering was stopped 10 to 20 days after cutting, and new shoots were induced from stem segments through drought stress. After the new shoots of the stems germinate, resume water and fer...

Embodiment 3

[0044] Collection of old stems

[0045] In May 2016, 20 2-year-old, bare, leafless, and slightly shrunken old stems were selected, and cut with scissors as cuttings, the length of the stem segment was 5-8 cm.

[0046] stem segment processing

[0047] Cut the incision end of the lower end of the old stem flat, save 0.5 cm from the stem at the lower end incision, and the length of the stem segment is 5-8 cm, and the incision is coated with 75% carbendazim powder.

[0048] stem cuttings

[0049] The three substrates (diameter: 2-3cm) of bark, stone and charcoal are mixed at a ratio of 2:1:1 as the cutting substrate, placed in a culture tray, and the treated old stems are placed flat on the surface of the substrate.

[0050] Management after insertion

[0051]Watering was stopped 10 to 20 days after cutting, and new shoots were induced from stem segments through drought stress. After the new shoots of the stems germinate, resume water and fertilizer management, water and f...

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Abstract

The invention discloses a cutting propagation method for splash-ink dendrobe old stems. The method comprises following steps: utilizing two-year or perennial splash-ink dendrobe pseudobulb as material; utilizing a high-temperature high-humidity condition for drought stress, forming buds induced on old stems, carrying out final-period management and seedling transplant to culture new excellent parental plant. The method is utilized for obtaining cuttage seedlings with excellent parental characters. From March to May, seedlings grow. From October to December, flowers bloom. New stem sprouts germinate for 12 to 25 days. Each pseudobulb has 1 to 4 buds of more than 90% germination rate. Compared with the aseptic sowing and tissue culture propagation, the method has following beneficial effects: the method is stable in inheritance, short in culture period, fast in florescence, low in production cost, easy in operation and easy in promotion; new plants can be cultured by effective utilization of aging pseudobulb so that nutrient consumption of new pseudobulb by utilizing aging pseudobulb is reduced and service lifetime of seedlings is prolonged; and therefore, a new way is provided for splash-ink dendrobe seedling propagation and effective utilization of old stems.

Description

technical field [0001] The invention belongs to the technical field of vegetative propagation of Dendrobium splashing ink, in particular to a cutting propagation method of old stems of Dendrobium splashing ink. Background technique [0002] "Pomo" Dendrobium (Dendrobium.Compactum White "Mutation") is a Dendrobium orchid hybrid introduced from Thailand in recent years. The petals are purple with white stripes, the number of flowers is 10-30, the flower diameter is 6-8cm, and the plant height is 20-30cm. The natural flowering period is May and September, and the group flowering period is 45-60 days. "Pomo" Dendrobium has a unique flower color, long flowering period and large amount of flowers. It is loved by the public and orchid lovers. It is currently a rare type of high-quality potted orchid. Dendrobium "Pomo" has been introduced for a short time in my country, and there are very few reports on reproduction. Currently, Dendrobium orchids are propagated mainly by aseptic se...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00
CPCA01G24/00A01G31/00
Inventor 龚建英王华新龙定建唐庆陈宝玲陈尔杨舒婷唐遒冥林茂李进华苏莉花
Owner GUANGXI FORESTRY RES INST
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