Cutting propagation method for splash-ink dendrobe old stems
A technology of cutting propagation and old stems, which is applied in the field of asexual propagation of Dendrobium nobile, can solve the problems of ornamental value and economic value decline, high technical content, virus accumulation, etc., and achieve high bud induction rate and survival rate, high genetic stability, The effect of extending the service life
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Embodiment 1
[0021] Collection of old stems
[0022] In August 2014, 20 2-year-old, bare, leafless, and slightly shrunken old stems were selected and cut with scissors as cuttings. The length of the stem segment was 5-8 cm.
[0023] The collection time of old stems should be selected after the temperature warms up in spring or in high temperature weather in summer and autumn. In winter, the temperature is low and it is difficult to induce new buds.
[0024] stem segment processing
[0025] Cut the incision end of the lower end of the old stem flat, save 0.5 cm from the stem at the lower end incision, and the length of the stem segment is 5-8 cm, and the incision is coated with 75% carbendazim powder.
[0026] stem cuttings
[0027] The three substrates (diameter: 2-3cm) of bark, stone and charcoal are mixed at a ratio of 2:1:1 as the cutting substrate, placed in a culture tray, and the treated old stems are placed flat on the surface of the substrate.
[0028] Management after inser...
Embodiment 2
[0033] Collection of old stems
[0034] In July 2015, 20 3-year-old to perennial, bare, leafless, and slightly shrunken middle and upper old stems were selected and cut with scissors as cuttings. The length of the stem segment was 5-8 cm.
[0035] stem segment processing
[0036] Cut the incision end of the lower end of the old stem flat, save 0.5 cm from the stem at the lower end incision, and the length of the stem segment is 5-8 cm, and the incision is coated with 75% carbendazim powder.
[0037] stem cuttings
[0038] The three substrates (diameter: 2-3cm) of bark, stone and charcoal are mixed at a ratio of 2:1:1 as the cutting substrate, placed in a culture tray, and the treated old stems are placed flat on the surface of the substrate.
[0039] Management after insertion
[0040] Watering was stopped 10 to 20 days after cutting, and new shoots were induced from stem segments through drought stress. After the new shoots of the stems germinate, resume water and fer...
Embodiment 3
[0044] Collection of old stems
[0045] In May 2016, 20 2-year-old, bare, leafless, and slightly shrunken old stems were selected, and cut with scissors as cuttings, the length of the stem segment was 5-8 cm.
[0046] stem segment processing
[0047] Cut the incision end of the lower end of the old stem flat, save 0.5 cm from the stem at the lower end incision, and the length of the stem segment is 5-8 cm, and the incision is coated with 75% carbendazim powder.
[0048] stem cuttings
[0049] The three substrates (diameter: 2-3cm) of bark, stone and charcoal are mixed at a ratio of 2:1:1 as the cutting substrate, placed in a culture tray, and the treated old stems are placed flat on the surface of the substrate.
[0050] Management after insertion
[0051]Watering was stopped 10 to 20 days after cutting, and new shoots were induced from stem segments through drought stress. After the new shoots of the stems germinate, resume water and fertilizer management, water and f...
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