Key genes, microRNAs and other non-coding RNAs or combination thereof used for identifying or regulating cell pluripotency
A pluripotent, non-coding technology, applied in the field of identifying or regulating key genes, microRNAs, other non-coding RNAs or their combinations for cell pluripotency, can solve the problem of not being able to verify the pluripotency and success rate of human iPS cells Low and slow down the development and application of iPS technology
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[0023] The inventor's research is based on rigorous animal experiments, identifying and classifying embryonic stem cells and pluripotent stem cells with different developmental potentials, and tracing the differences between these pluripotent stem cells through large-scale Solexa sequencing and gene chip data, thereby It was found that genes, microRNAs and other non-coding RNAs located in the Dlk1-Dio3 segment were highly expressed in fully pluripotent cells with the highest developmental potential, but were significantly suppressed or silenced in incomplete pluripotent stem cells with lower developmental potential; through The results obtained by detecting this cluster of genes and non-coding small RNAs and then verifying pluripotency are also completely consistent with this conclusion. Thus, in mice, the expression of this cluster of genes is an important marker of stem cell pluripotency.
[0024] MicroRNA (miRNA) refers to a short chain of up to 22 nucleotides whose precurs...
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