Aspergillus aculeatus strain and application thereof
A technology of Aspergillus aculeatus and strains, applied in the fields of fermentation engineering and biology, can solve the problem of low activity of β-glucosidase, and achieve the effects of reasonable design, easy process control and high enzyme activity
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[0022] The technical solution of the present invention will be further specifically described below through specific examples.
[0023] 1. Isolation and purification of bacterial strains
[0024] 1.1 Sample collection: 25 soil samples were collected in sterile test tubes from the soil under drainage ditches of small bamboo forests, wild tea trees, rotten bamboo leaves and dead peach trees.
[0025] 1.2 Sample processing
[0026] Reagent preparation: sample treatment solution containing antibiotics configuration: ampicillin 1g, kanamycin 0.55g, sodium chloride 8.5g, Tween 1g dissolved in appropriate amount of water; 1g chloramphenicol dissolved in 4.5ml ethanol; ethanol-chlorine The mycin solution was mixed into the solution obtained in the first step, and the volume was adjusted to 1 L to obtain a treatment solution.
[0027] Screening medium: Caspian medium containing methyl red (g / L): CMC-Na 15.0, sodium nitrate 3.0g, magnesium sulfate heptahydrate 0.5g, potassium chloride...
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Description
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Application Information
- IPC
- C12N1/14; C12N9/42; C12R1/66
- CPC
- C12N9/2445; C12Y302/01021; C12N1/145; C12R2001/66
- Inventors
- 袁辉
