A kind of method utilizing Aspergillus aculeatus to prepare steviol and purify rebaudioside A

A technology of Aspergillus aculeatus and steviol, which is applied in biochemical equipment and methods, microorganism-based methods, microorganisms and other directions, can solve the problems of other uses of strains not mentioned, and achieves low separation cost, simple process and good quality. Effect

Active Publication Date: 2014-10-15
巴州正达绿源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

and a method for preparing 5,7,8,4'-tetrahydroxyisoflavones with the strain, but no other uses of the strain are mentioned

Method used

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  • A kind of method utilizing Aspergillus aculeatus to prepare steviol and purify rebaudioside A
  • A kind of method utilizing Aspergillus aculeatus to prepare steviol and purify rebaudioside A
  • A kind of method utilizing Aspergillus aculeatus to prepare steviol and purify rebaudioside A

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Use conventional Aspergillus aculeatus solid koji culture medium, add water after fermentation to obtain Aspergillus aculeatus enzyme liquid, add 30ml each time

[0034] The substrate was 1.0% (w / v) steviol glycoside, transformed in a water bath at 45°C for 24 hours, separated and purified to obtain steviol and RA.

Embodiment 2

[0036]Use conventional Aspergillus aculeatus solid koji culture medium, add water to extract after fermentation to obtain Aspergillus aculeatus enzyme liquid, add 100ml of substrate with 15% (w / v) steviol glycoside, transform in 50°C water bath for 48h, separate and purify to obtain stevia Alcohol and RA.

Embodiment 3

[0038] Use conventional Aspergillus aculeatus solid koji culture medium, after fermentation, add water to extract Aspergillus aculeatus enzyme liquid, add 7% (w / v) steviol glycoside as a substrate in 100ml, transform in a water bath at 50°C for 40h, separate and purify to obtain stevia Alcohol and RA.

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Abstract

The invention relates to a method for converting stevioside or rubusoside raw materials into steviol by utilizing Aspergillusaculeatus ZJ (the preservation number of which is CCTCC No:M2011264) bacterium and enzymes thereof, and purifying rebaudioside A (RA). The method specially comprises the steps of: acting Aspergillusaculeatus or fermentation liquor thereof or extracted enzyme liquor on the stevioside or stevia rabaudiana extract liquid, and converting ingredients, except RA, of the stevioside to product steviol; settling and separating the steviol from the solution; purifying and separating by adopting a conventional crystallization and other purification methods, wherein the RA in the system is not converted and left in the solution; and purifying the RA by adopting the following convention extracting, separating and purifying method. The invention provides a method for converting stevioside into steviol by utilizing Aspergillusaculeatus bacterium and enzyme liquor thereof, and purifying rebaudioside A. According to the method, the conditions are mild, the environment compatibility is good, and the process equipment is simple, thus application values are high.

Description

technical field [0001] The present invention relates to a most effective new method for simultaneous preparation of steviol and purification of rebaudioside A (RA), especially the use of enzyme liquid produced by microbial fermentation to convert stevioside or rubusoside raw materials or their derivatives into steviol The method belongs to the field of biotransformation of natural products. Background technique [0002] steviol [0003] Molecular formula: C 20 h 30 o 3 [0004] Molecular Structure: [0005] [0006] Steviol (ent-13-hydroxykaur-16-en-19-oic acid, steviol) is an aglycone of stevioside, and the molecule has an enantiokaurene-type tetracyclic diterpene skeleton structure, and an enantiokaurene-type diterpene Most of these compounds have a wide range of physiological activities, such as heat-clearing and detoxifying, anti-bacterial and anti-inflammatory, anti-tumor effects (Hu Yanqiu, Shi Hao. Research progress of steviol and its derivatives. Zhejiang Ch...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/42C12P19/56C12R1/66
Inventor 陈育如马迎迎闫莽
Owner 巴州正达绿源生物科技有限公司
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