A strain of Aspergillus aculeatus and method for preparing 5,7,8,4'-tetrahydroxyisoflavones using the strain
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A technology of hydroxyisoflavones and Aspergillus aculeatus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the problems of high separation cost and low content, and achieve abundant resources, high yield, and transformation high efficiency effect
Active Publication Date: 2011-12-14
NANJING NORMAL UNIVERSITY
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Most of the existing studies obtained 5,7,8,4'-tetrahydroxyisoflavones from soybean fermentation products. TE-SHENG, CHANG used Aspergillus niger to extract 33.5mg of 5,7 from 500g soybean germ solid koji. 8,4'-Tetrahydroxyisoflavone (Te-Sheng Chang. Mushroom tyrosinase inhibitory effct isofisoavone sisolated from soygerm koji fermented with Aspergillus oryzae BCRC32288[J]. Food Chemistry, 2007, (105): 1430-1438), the yie
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[0041] Preparation of Aspergillus aculeatus powder or enzyme powder: The bacteria powder or enzyme powder can be obtained by conventional liquid or solid fermentation culture and enzyme separation method.
[0042] For example, the preparation of bacteria powder includes fermentation of bacteria, separation of fermentation broth, emulsification of bacteria sludge and vacuum freeze-drying of emulsion. Enzyme powder was obtained by freezing the enzyme solution fermented by Aspergillus aculeatus and drying it in vacuum for 30 h at a vacuum degree of 1-5 Pa.
[0043] The material used in the present invention is the conversion substrate: picked from the campus of Nanjing Normal University in Nanjing, Jiangsu.
Embodiment 1
[0045] Example 1: Prepare 10% conversion stock solution pH5, see HPLC test results figure 2 , inoculate the Aspergillus aculeatus solution at 5% inoculum amount, culture at 30°C for 4 days, centrifuge at 8000rpm after shaking the flask, take the supernatant, and see the HPLC test results in image 3 As shown, the solid product of 5,7,8,4’-tetrahydroxyisoflavone was obtained by the conventional extraction method with a purity of 98%. The HPLC test results are shown in Figure 4 shown.
Embodiment 2
[0046] Example 2: Take appropriate amount of Sophora japonicus powder, add appropriate amount of water, add 10% (W / W) bacterial solution, and transform at 30°C for 3.5 days. After separation and purification, high-purity 5,7,8,4'-tetrahydroxyisoflavones were obtained.
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Abstract
The invention relates to an Aspergillus aculeatus ZJ bacterial strain and an application thereof. The collection number of the Aspergillus aculeatus ZJ is CCTCC NO.M2011264. The bacterial strain is mainly used for converting effective components of the pod of Chinese scholartree to produce 5,7,8,4'-tetrahydroxyisoflavone. The specific method provided by the invention comprises the following steps of: acting the Aspergillus aculeatus ZJ with strong specificity or the produced enzyme on the pod of Chinese scholartree or extracting solution thereof; converting glucosides compounds including sophoricoside and the like, genistein and the like to produce the 5,7,8,4'-tetrahydroxyisoflavone. The invention provides the method for producing the 5,7,8,4'-tetrahydroxyisoflavone through utilizing the Aspergillus aculeatus with high specificity and high conversion efficiency to convert the sophoricoside; and the method provided by the invention has moderate conditions, green process and simple requirements on the process and the device, and has a very good application value.
Description
technical field [0001] The present invention relates to a new method for preparing 5,7,8,4'-tetrahydroxyisoflavones, in particular, using microorganisms or enzymes to convert genistein and its glycosides into 5,7,8,4'-tetrahydroxyisoflavones Methods. It belongs to the field of research and development of new traditional Chinese medicine. Background technique [0002] 5,7,8,4'-Tetrahydroxyisoflavone [0003] Molecular formula: C 15 h 10 o 6 [0004] Molecular weight: 286 [0005] Molecular Structure: [0006] . [0007] 5,7,8,4'-Tetrahydroxyisoflavone, yellowish solid, soluble in methanol, ethanol, ethyl acetate and other organic solvents, hardly soluble in water. 5,7,8,4'-Tetrahydroxyisoflavone belongs to isoflavones, which is a derivative of genistein with a hydroxyl group added. [0008] Genistein is an estrogen-like compound drug, known as phytoestrogens, and is mainly used in medicine to treat gynecological diseases and senile osteoporosis (Chinese patent CN...
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