A kind of Paenibacillus polymyxa, its application in the preparation of genistein and the method for preparing genistein

A polymyx spore-like and genistein technology is applied in the field of research and development of new traditional Chinese medicines, which can solve problems such as undiscovered transformation research, and achieve the effects of strong transformation specificity, high transformation efficiency and abundant resources.

Active Publication Date: 2020-10-09
南京师范大学泰州学院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research on the transformation of Paenibacillus polymyxa to genistin in Sophora japonicus

Method used

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  • A kind of Paenibacillus polymyxa, its application in the preparation of genistein and the method for preparing genistein
  • A kind of Paenibacillus polymyxa, its application in the preparation of genistein and the method for preparing genistein
  • A kind of Paenibacillus polymyxa, its application in the preparation of genistein and the method for preparing genistein

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preparation example Construction

[0061] The preparation method of the Sophora japonicus extract in the present invention: take the solid Sophora japonicus powder, fully extract with water according to the solid-liquid ratio, and then filter to obtain the extract. Taking 10% W / V Sophora japonica extract as an example, its preparation method is as follows: take 10 g of solid Sophora japonica powder, fully boil and extract it with 100 ml of water, filter and take the filtrate to dilute to 100 ml.

[0062] The preparation method of the Paenibacillus polymyxa bacteria liquid in the present invention: transfer the strain stored in the refrigerator to a PDA slope for activation, cultivate at 37°C for 36 hours, transfer to the fermentation medium, and shake and cultivate for 2 days at 37°C and 220r / min on a shaking table That is, the bacterial liquid is obtained.

[0063] The preparation method of Paenibacillus polymyxa bacterial powder in the present invention: transfer the bacterial strains stored in the refrigerat...

Embodiment 1

[0073] Prepare 10% W / V, pH5 Sophora japonica extract, inoculate the Paenibacillus polymyxa bacterial solution of the present invention into the above-mentioned transformation substrate according to the inoculum amount of 5% (V / V) for transformation.

[0074] Transformation method: Shake flask culture at 37°C for 4 days.

[0075] After the conversion, centrifuge at 8000rpm, take the supernatant, and obtain the solid product of genistein by conventional extraction methods.

[0076] The content of genistin and genistein before and after conversion was detected by HPLC method. The result is as image 3 and Figure 4 shown.

[0077] figure 2 It is the standard HPLC figure of genistin and genistein. Wherein A is genistin, the retention time is 10.797min, B is genistein, the retention time is 19.01min.

[0078] image 3 It is the HPLC chart of the conversion stock solution of the present invention.

[0079] Figure 4 It is the HPLC figure after conversion of the present inv...

Embodiment 2-19

[0086] The biotransformation process of Examples 2-19 is basically the same as Example 1.

[0087] The addition form, dosage, transformation substrate and reaction conditions of the transformation method of the microorganisms used are slightly different, as shown in the table below:

[0088]

[0089]

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Abstract

The invention provides a Paenibacillus polymyxa ( Paenibacillus polymyxa ), and its deposit number is CCTCC No.M2018877. Also provided is an application of the Paenibacillus polymyxa in preparing genistein. Also provided is a method for preparing genistein by using the Paenibacillus polymyxa. The present invention has the following technical effects: 1) The present invention discovers Paenibacillus polymyxa capable of converting genistein into genistein for the first time, which has new properties compared with similar Paenibacillus polymyxa; 2) This invention The invention has strong transformation specificity and high transformation efficiency, which can reach 100%. 3) In the method of the present invention, the raw material Sophora japonicus used is rich in resources, cheap and easy to obtain, and is an ideal raw material for preparing genistein.

Description

technical field [0001] The invention relates to Paenibacillus polymyxa and its application in preparing genistein and a method for preparing genistein, belonging to the field of research and development of new traditional Chinese medicines. Background technique [0002] Molecular formula of genistein: C 15 h 10 o 5 [0003] Molecular weight: 270.23 [0004] Molecular Structure: [0005] [0006] Genistein is an estrogen-like compound drug, known as phytoestrogens, and is mainly used clinically to treat gynecological diseases and senile osteoporosis (Chinese patent CN101497594A). In addition, in anti-tumor (SanjeevBanerjee et al, 2005, Molecular evidence for increased antitum oractivity of gemcitabine by genistein invitro and invivo using an or the topic model of Pancreatic cancer [J]. Cancer Res. 2005, 65:9064-9072), anti-inflammatory, Labor pain, lowering uric acid (Huang Jingqun et al. Experimental study on anti-inflammatory, analgesic, and lowering uric acid effe...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P17/06C12R1/01
CPCC12P17/06C12N1/205C12R2001/01
Inventor 张玉千周学郑丹张宇
Owner 南京师范大学泰州学院
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