72 results about "Aspergillus variecolor" patented technology

The invention relates to a method for preparing high purity galacto-oligosaccharides, comprising the following product separation and purification steps: Aspergillus oryzae fermentation, ceramic membrane ultrafiltration, nanofiltration separation and the like. In the method, the Aspergillus oryzae separated from the soil is adopted as an original strain and the Aspergillus oryzae BLB-21 (with preservation number of CGMCC No.2951), a high efficiency transformed strain obtained through mutation screening in the laboratory, is directly used to ferment high concentration lactose solution, thus avoiding the steps of enzyme purification and the like in the process of preparing the galacto-oligosaccharides by an enzyme method and saving time and labor. The high purity galacto-oligosaccharides are obtained by ultrafiltration and nanofiltration separation, the process is ideal, the conditions are mild and the method has extensive industrial production prospect.

The invention relates to an Aspergillus aculeatus ZJ bacterial strain and an application thereof. The collection number of the Aspergillus aculeatus ZJ is CCTCC NO.M2011264. The bacterial strain is mainly used for converting effective components of the pod of Chinese scholartree to produce 5,7,8,4'-tetrahydroxyisoflavone. The specific method provided by the invention comprises the following steps of: acting the Aspergillus aculeatus ZJ with strong specificity or the produced enzyme on the pod of Chinese scholartree or extracting solution thereof; converting glucosides compounds including sophoricoside and the like, genistein and the like to produce the 5,7,8,4'-tetrahydroxyisoflavone. The invention provides the method for producing the 5,7,8,4'-tetrahydroxyisoflavone through utilizing the Aspergillus aculeatus with high specificity and high conversion efficiency to convert the sophoricoside; and the method provided by the invention has moderate conditions, green process and simple requirements on the process and the device, and has a very good application value.

The invention relates to expression of improved rabbit defensin NP-1 (mNP-1) employing chlorella. The defensin mNP-1 expressed in the chlorella has a high inhibiting or killing effect. The result of the defensin can be applied to preparation of medicines for resisting aspergillus.

The invention discloses a method for synthesizing xylitol by aspergillus oryzae engineering bacteria with enhanced hemicellulose saccharification capacity. The method comprises the steps of 1) obtaining an aspergillus oryzae strain; 2) deleting an orotic acid nucleoside-5'-phosphate decarboxylase gene (pyrG) as a selection marker by utilizing a gene homologous recombination technology, constructing an uridine auxotroph homologous transformation system based on pyrG deletion, and providing uridine auxotroph host bacteria for subsequent gene modification; and 3) carrying out traceless deletion on the xdh gene by using a gene deletion technology of which the selection marker can be recycled to obtain the xylitol CBP engineering bacteria with enhanced hemicellulose saccharification capacity.

The invention discloses a genetically engineered bacterium with high yield of trans-aconitic acid as well as a construction method and application of the genetically engineered bacterium, and belongsto the field of genetic engineering. Chemical synthesis of trans-aconitic acid has the defects of more byproducts and complex process. Aiming to overcome the production defects of the trans-aconitic acid, the invention constructs the genetically engineered bacterium for producing the trans-aconitic acid. The method comprises the following steps: subjecting Cytochrome P450 monooxygenase gene CICC_3028g in At-delta cadA aspergillus terreus to gene mutation to obtain recombinant aspergillus terreus. The content of the trans-aconitic acid produced by fermentation of the recombinant aspergillus terreus is remarkably increased compared with that of trans-aconitic acid produced by the At-delta cadA aspergillus terreus.

The present invention relates to a mutant polypeptide derived from aspergillus terreus NIH2624 transaminase (XP-001209325.1), a polynucleotide encoding the polypeptide, and an application of the polypeptide in the preparation of (R)-1-BOC-3-aminopiperidine. The transaminase is directionally modified through computer simulation, and modified amino acid sites are selected from one or more of Y60, V62, F115, W184, G216, F217, N218, L235, G237, V238, T239, C273, T274, T275 and A276. The mutant obtained by modification is applied to the production of (R)-1-BOC-3-aminopiperidine, has the advantagesof good stereoselectivity and catalytic activity, mild catalytic reaction, improvement of the activity by 61.9 times compared with wild transaminase under the same production conditions, obvious improvement of the chiral purity and yield of the product, and good application prospect.

The invention discloses an aspergillus oryzae functional enzyme preparation and application thereof, and belongs to the technical field of white wine brewing. The aspergillus oryzae functional enzyme preparation is prepared by performing solid state fermentation and drying on an aspergillus oryzae strain. In the white wine brewing process, the aspergillus oryzae functional enzyme preparation is additionally added into cooked and gelatinized main raw materials to be fermented. As the aspergillus oryzae functional enzyme preparation is added to ferment the raw materials, the process is simple and controllable, the wine yield and the high-quality rate of white wine can be effectively increased, the quality of the white wine is improved, and social and economic benefits are achieved.

The invention provides preparation of an amylase-rich compound enzyme as well as a strain and application of the compound enzyme. A cultured fermentation product contains amylase, neutral proteases, xylanases, cellulases, glucanases and pectinases. Aspergillus oryzae is named as aspergillus oryzae BAK200312, and the preservation number of the Aspergillus oryzae is CGMCC No.19617. The invention provides a rich complex enzyme system. The used aspergillus oryzae strain is obtained by strict domestication, has strong enzyme production capability, high amylase yield and high enzyme activity, and isan aspergillus oryzae strain with efficient amylase production capability.

The invention discloses a method for producing chitosanase by using Aspergillus usamii, which belongs to the technical field of biological engineering. In the method, agricultural and sideline products such as bran, soybean cake powder and rapeseed cake powder are used as fermentation substrates, a proper amount of inorganic nitrogen source, chitosan, inorganic salt, surfactant and the like are added, and the chitosanase is produced by the solid fermentation of an Aspergillus usamii E001 strain. The invention provides the formula and culture conditions of a solid fermentation culture medium for test production of chitosanase by the Aspergillus usamii E001 in a laboratory, and the chitosanase activity of a mature fermented grain material of the medium reaches 1,942 to 2,461U/g. The method has the advantages of simple equipment, small investment, quick effectiveness, low production cost, environmentally-friendly, high activity of chitosanase fermentation enzyme, and the like, contributes to the development and utilization of chitosanase and has high economic and practical value.

Provided are an aspergillus oryzae BLCY-006 strain and an application thereof in the preparation of a galactooligosaccharide. The strain produces β-galactosidase, and the enzyme activity can reach 300 U/ml after culturing and fermentation, which is more than 50% higher than traditional β-galactosidase activity. The enzyme also has lactose and glucose resistance properties.

The invention discloses a method for improving production capacity of Aspergillus niger saccharifying enzyme and a recombinant Aspergillus niger strain. The method comprises the following steps: integrating uracil defective (pyrG-) strains with high yield of aspergillus niger saccharifying enzyme by using a Tet-on system through a promoter of an exogenous plasmid and utilizing homologous recombination, so that at least one of acyl-coenzyme A dehydrogenase coding genes, acetyl-coenzyme A acyltransferase coding genes or cytochrome P450 monooxygenase coding genes is over-expressed; and the saccharifying enzyme production capacity of the aspergillus niger is greatly improved by regulating and controlling a fatty acid metabolic pathway.

The invention provides a solid-state fermentation wheat bran feed, and a preparation method and an application thereof. The feed comprises the following raw materials in parts by weight: 1-3 parts ofwheat bran, 1.2-3.2 parts of a nutrient solution, 0.15-0.45 part of an aspergillus niger-trichoderma viride-streptomyces rouxii composite bacterial suspension and 0.05-0.15 part of a candida tropicalis suspension. The preparation method comprises the following steps: respectively activating and culturing aspergillus niger, trichoderma viride, streptomyces rouxii and candida tropicalis, then compounding the aspergillus niger, the trichoderma viride and the streptomyces rouxii, mixing an obtained mixture with wheat bran for primary fermentation, and adding candida tropicalis for secondary fermentation after completion of primary fermentation. According to the invention, by selection of the streptomyces rouxii, the aspergillus niger and the trichoderma viride, abundant enzymes like cellulase,pectinase and amylase can be generated in the growth process, so components like cellulose uneasily utilized by animals in wheat bran can be easily degraded; meanwhile, the candida tropicalis can berapidly propagated by utilizing a fermentation product, namely reducing sugar in the later period of fermentation, and a large amount of mycoprotein is generated at the same time; and through synergistic effect of the streptomyces rouxii, the aspergillus niger and the trichoderma viride, the content of cellulose in the feed can be effectively reduced.

The invention discloses a preparation and application of a transeliminase encoding gene derived from Aspergillus parasiticus, and an enzyme of the transeliminase encoding gene, that is, a gene of transeliminase is cloned to a pichia pastoris expression vector by using a technical method of gene engineering, then a pichia pastoris recombinant strain for heterologous expression of the enzyme can beobtained, the strain is capable of heterologously expressing the prepared transeliminase and efficiently degrading pectin polysaccharides and polygalacturonic acid (PGA). The transeliminase disclosedby the invention can be widely applied to fields such as agriculture, industry, foods, feed additives, medicines and pectin oligosaccharide preparation.