Method for producing chitosanase by using Aspergillus usamii

Abstract

The invention discloses a method for producing chitosanase by using Aspergillus usamii, which belongs to the technical field of biological engineering. In the method, agricultural and sideline products such as bran, soybean cake powder and rapeseed cake powder are used as fermentation substrates, a proper amount of inorganic nitrogen source, chitosan, inorganic salt, surfactant and the like are added, and the chitosanase is produced by the solid fermentation of an Aspergillus usamii E001 strain. The invention provides the formula and culture conditions of a solid fermentation culture medium for test production of chitosanase by the Aspergillus usamii E001 in a laboratory, and the chitosanase activity of a mature fermented grain material of the medium reaches 1,942 to 2,461U / g. The method has the advantages of simple equipment, small investment, quick effectiveness, low production cost, environmentally-friendly, high activity of chitosanase fermentation enzyme, and the like, contributes to the development and utilization of chitosanase and has high economic and practical value.

Description

technical field

[0001] The invention relates to a method for producing chitosanase by using Aspergillus usamii, in particular to a new process for producing chitosanase by solid-state fermentation of Aspergillus usamii E001 strain, which belongs to the technical field of bioengineering. Background technique

[0002] Chitosan is the deacetylation product of chitin, and it is a polymer cationic flocculant. The chitosan oligosaccharide obtained by partial hydrolysis of chitosan has good water solubility, non-toxicity, no pyrogen, no variability, easy to be absorbed by biological organisms, and has unique physiological activity. Wide application prospects. Using chitosan, a biocatalyst with high specificity, to hydrolyze chitosan, it has the characteristics of mild reaction conditions, easy control of the reaction process, no pollution to the environment, the highest yield of chitosan oligosaccharides, and stable quality. It is an ideal production method. Chitooligosaccharide ...