Solid-state fermentation wheat bran feed, and preparation method and application thereof

A technology of solid-state fermentation and wheat bran, which is applied in the direction of biochemical equipment and methods, applications, animal feed, etc., can solve the problems of ineffective degradation of wheat bran, limited application, and poor cellulose decomposition effect, so as to improve the application value and reduce the Diarrhea rate, effect of increasing content

Active Publication Date: 2020-04-10
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, fermented wheat bran is mostly fermented by a single strain. This kind of fermented wheat bran can greatly increase its protein content, especially soluble protein content, but it has poor decomposition effect on refractory substances such as cellulose, and cannot effectively degrade wheat bran. its application in animal husbandry

Method used

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  • Solid-state fermentation wheat bran feed, and preparation method and application thereof
  • Solid-state fermentation wheat bran feed, and preparation method and application thereof
  • Solid-state fermentation wheat bran feed, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Screening of Fermentation Strain Combinations Optimum for Cellulose Degradation

[0040] The present invention selects 3 kinds of bacterial strains (Aspergillus niger (H), Trichoderma viride (L), Neurospora crassa (C)) altogether. There are 7 combinations, they are H, L, C, HL, HC, LC, HLC, each combination is inoculated in solid medium according to the orthogonal design table to ferment wheat bran, the inoculation amount, material-water ratio, fermentation Time and fermentation temperature are the fermentation factors, and each factor is set to 3 levels, using L 9 ( 3 4) Orthogonal table (Table 2) An orthogonal fermentation experiment with four factors and three levels was carried out, with 9 groups in total, and each group had 3 repetitions. Orthogonal experimental design is shown in Table 1.

[0041] Table 1 Orthogonal experimental design

[0042]

[0043] Note: A represents the inoculation amount (0%), B represents the material-water ratio (W / V), C represents...

Embodiment 2

[0061] Screening of the Combination of Fermentation Strains Optimum for Producing Somatic Protein

[0062] The above-mentioned combinations with the top three cellulase activities were used to re-ferment the wheat bran according to the test conditions of Example 1, and immediately after the fermentation was completed, the Candida tropicalis suspension was added and mixed, and at the same time, it was fermented according to the same test conditions 1d, the combination with the highest crude protein content and the lowest crude fiber content in the obtained fermentation product is the optimum fermentation combination.

[0063] The changes in the amount of yeast suspension added, crude protein, crude fiber, etc. are shown in Table 7.

[0064] Table 7 Changes of crude protein and crude fiber content before and after solid-state fermentation of wheat bran

[0065]

[0066] Note: "-" means not added or none.

[0067] It can be seen from Table 7 that compared with unfermented wh...

Embodiment 3

[0075] After selecting the strain combination (HLC6+J), the present embodiment provides a solid-state fermented wheat bran feed, which consists of: 1 part of wheat bran, 1.2 parts of nutrient solution, Aspergillus niger-Trichoderma viride-rough 0.15 part of compound bacterial culture solution of Neurospora, 0.05 part of Candida tropicalis suspension, wherein, the composition of described nutrient solution is: every 1L distilled water contains (NH 4 ) 2 SO 4 5g, KH 2 PO 4 1g, CaCl 2 0.6g, MgSO 4 ·7H 2 O 0.5g, FeSO 4 ·7H 2 O 0.5g, ZnSO 4 0.5g, MnSO 4 0.5g, CoCl 2 ·6H 2 O 0.5 g. The composite culture liquid of described Aspergillus niger-Trichoderma viride-Neurospora crassa consists of: every 1L of distilled water contains Aspergillus niger suspension 1mL, Trichoderma viride suspension 1mL, Neurospora crassa suspension 1mL, glucose 10g, soluble starch 15g, peptone 0.75g, KH 2 PO 4 1.5g, (NH 4 ) 2 SO 4 2.5g, MgSO 40.75g. The number of spores in the compou...

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Abstract

The invention provides a solid-state fermentation wheat bran feed, and a preparation method and an application thereof. The feed comprises the following raw materials in parts by weight: 1-3 parts ofwheat bran, 1.2-3.2 parts of a nutrient solution, 0.15-0.45 part of an aspergillus niger-trichoderma viride-streptomyces rouxii composite bacterial suspension and 0.05-0.15 part of a candida tropicalis suspension. The preparation method comprises the following steps: respectively activating and culturing aspergillus niger, trichoderma viride, streptomyces rouxii and candida tropicalis, then compounding the aspergillus niger, the trichoderma viride and the streptomyces rouxii, mixing an obtained mixture with wheat bran for primary fermentation, and adding candida tropicalis for secondary fermentation after completion of primary fermentation. According to the invention, by selection of the streptomyces rouxii, the aspergillus niger and the trichoderma viride, abundant enzymes like cellulase,pectinase and amylase can be generated in the growth process, so components like cellulose uneasily utilized by animals in wheat bran can be easily degraded; meanwhile, the candida tropicalis can berapidly propagated by utilizing a fermentation product, namely reducing sugar in the later period of fermentation, and a large amount of mycoprotein is generated at the same time; and through synergistic effect of the streptomyces rouxii, the aspergillus niger and the trichoderma viride, the content of cellulose in the feed can be effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of feed preparation, and in particular relates to a solid-state fermented wheat bran feed and a preparation method and application thereof. Background technique [0002] In recent years, with the increasing population and decreasing arable land area, the shortage of feed raw materials has become more and more serious, which seriously restricts the development of my country's animal husbandry industry. At present, my country's feed accounts for about 35% of the total grain output, and it is estimated that by 2020 and 2030, this proportion will reach 45 and 50% respectively. However, the expected increment of grain is only about 1% every year, and the shortage of feed grain is inevitable. Therefore, improving the utilization rate of my country's existing feed resources is the key link to alleviate this contradiction. Wheat bran is the main by-product of the milling industry. The annual output of wheat in my ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/37A23K50/30A23K50/60A23K20/26A23K20/24A23K20/20C12N1/16C12N1/14C12R1/685C12R1/885C12R1/74C12R1/645
CPCA23K10/12A23K10/37A23K50/30A23K50/60A23K20/26A23K20/24A23K20/30A23K20/20C12N1/16C12N1/14Y02P60/87
Inventor 罗玉衡何俊兰聪李华陈洪田刚陈代文
Owner SICHUAN AGRI UNIV
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