31 results about "Sophoricoside" patented technology

The invention relates to a herbal drug for hemorrhoids with natural plants, in particular to a drug with specific formulation, comprising a plurality of active pharmaceutical ingredients, certain amount of penetration enhancer and certain amount of pharmacyeutical adjuvant with the following weight proportion. The active pharmaceutical ingredient comprises one hundred shares of sophoricoside, eighty shares of panax notoginseng, one hundred and ten shares of lightyellow sophora root, one hundred shares of bletilla striata, one hundred shares of common cnidium fruit, one hundred shares of patrinia scabiosaefolia link, sixty-five shares of moxa leaf, eighty shares of pursslane, sixty-five shares of honeysuckle, sixty-five shares of parsnip, fifty shares of alumen, fifty shares of borax, five shares of borneol, and thirty shares of licorice. The penetration enhancer comprises a certain amount of laurocapram and a certain amount of propanediol with the weight proportion of eighty to fifteen - thirty; the adding amount of the penetration enhancer is point zero eight to point four percent of the weight of the active pharmaceutical ingredient. The drug has the advantage of being more effective than oral drugs and external lotion due to full play of effective ingredients by adding penetration enhancer in specific prescription and formulation.

The invention discloses a method for extracting flavone ingredients and extracting kaempferide from sophoricoside and relates to the medicine. The method comprises the following steps: de-ionized water is added into the raw material of the sophoricoside, and reflux extracting is carried out in heating condition; extracting liquid-purpose diatomite and filtering is carried out; the filter solution is put on a macroporous resin column, impurity is washed by water and the filter solution is eluted by high-concentration ethanol; concentrating and drying is carried out on the eluting solution, thus obtaining the sophoricoside flavone extract; hydrolysate is added into the sophoricoside flavone extract and hydrolyzing is carried out in heating condition; pH value is adjusted to neutral after hydrolysis; steaming and removing alcohol, filtering, washing repeatedly by water are carried out on filter residue; drying and extracting are carried out on the filter residue by an extracting agent of mixed solution of aether and ethanol, the extract solution is steamed and the high-purity kaempferide is obtained. The method can be used for producing high-purity kaempferide flavone extract and kaempferide and has the advantages of simple process, good product quality, high degree of comprehensive utilization, being suitable for industrial production and low cost.

The present invention discloses Rhizopus oryzae LJH3 and applications of the Rhizopus oryzae LJH3 in preparation of genistein through biotransformation of sophoricoside. According to the present invention, the applications comprise that a fermentation broth obtained by carrying out fermentation culture on Rhizopus oryzae LJH3, or the mixed liquid of a filtrate obtained by filtering out bacteria from the fermentation broth and an equal volume of distilled water is used as a biological catalyst and sophoricoside is used as a substrate to form a transformation system, a transformation reaction is performed at a temperature of 30-38 DEG C under a 200-250 r / min constant temperature oscillation condition, and the transformation liquid is separated and purified to obtain the genistein after the transformation reaction is completed; the transformation yield of the genistein is 84.2% and the purity is 92.5% when the substrate feeding concentration is 1 g / L; and the low-activity sophoricoside is transformed into the good activity genistein through the method, and the method is the optional genistein production method, and has advantages of low production cost, high transformation rate, low environmental pollution, and the like.

The invention relates to an Aspergillus aculeatus ZJ bacterial strain and an application thereof. The collection number of the Aspergillus aculeatus ZJ is CCTCC NO.M2011264. The bacterial strain is mainly used for converting effective components of the pod of Chinese scholartree to produce 5,7,8,4'-tetrahydroxyisoflavone. The specific method provided by the invention comprises the following steps of: acting the Aspergillus aculeatus ZJ with strong specificity or the produced enzyme on the pod of Chinese scholartree or extracting solution thereof; converting glucosides compounds including sophoricoside and the like, genistein and the like to produce the 5,7,8,4'-tetrahydroxyisoflavone. The invention provides the method for producing the 5,7,8,4'-tetrahydroxyisoflavone through utilizing the Aspergillus aculeatus with high specificity and high conversion efficiency to convert the sophoricoside; and the method provided by the invention has moderate conditions, green process and simple requirements on the process and the device, and has a very good application value.

The invention discloses a novel technology for extracting genistein by using sophoricoside as a raw material, comprising the following steps of: crushing sophoricoside, heating by water and carrying out reflux extraction; concentrating a water decoction to an extract, followed by reflux and hydrolysis; cooling and filtering to obtain a crude product; carrying out recrystallization on the crude product to obtain the genistein with the purity being over 98%. The invention has the following advantages: the price of raw materials is low, the product output and high purity are high, the production cost is low, the technology is simple and easy to operate, the requirement on equipment is low and reagents are easy to recover.

The invention discloses a preparation method of a traditional Chinese medicine thermosensible gel for treating hemorrhoids. The preparation method comprises the following steps: weighing the raw materials in percentage by weight: 10-40 percent of panax notoginseng, 5-20 percent of cortex moutan, 5-20 percent of phellodendron, 5-20 percent of sophora flavescens ait, 5-15 percent of sophoricoside, 5-15 percent of galla chinensis and 5-15 percent of borneol; crushing the panax notoginseng, the cortex moutan, the phellodendron, the sophora flavescens ait and the sophoricoside into coarse powder, putting the coarse powder in a percolating extraction pot, adding 70 percent ethyl alcohol, soaking, percolating, and carrying out vacuum concentration to obtain a liquid extract 1; putting the galla chinensis in a multifunctional extraction pot, adding water, decocting twice, combining filtrates, and carrying out vacuum concentration to obtain a liquid extract 2; combining the liquid extract 1 and the liquid extract 2, and spray drying to obtain an extract powder; putting the borneol in a crusher, and crushing into fine powder; mixing the extract powder and the borneol fine powder according to an equivalent increasing method; and according to the weight ratio, adding 30-50 parts of poloxamer 407, 1-5 parts of poloxamer 188, 0.4-1 part of benzalkonium bromide and 180 parts of purified water after the per 100 parts of medicinal materials are extracted, so as to prepare the thermosensible gel. According to the preparation method, as water and the ethyl alcohol are used as extraction solvents only in the whole technological process, no wastewater and waste gas are generated in the production process, and the traditional Chinese medicine thermosensible gel is suitable for industrial production.

The invention relates to a medicine that is a mongolian medicine for treating hemorrhoids. The medicine is made from raw ingredient medicines by weight parts as follows: sophoricoside is 30 to 35 parts, garden burnet is 15 to 20 parts, bitter orange is 15 to 20 parts, scutellaria is 15 to 20 parts, catnip is 15 to 20 parts, coptis is 15 to 20 parts, biota orientalis is 15 to 20 parts, radix saposhinikoviae is 8 to 12 parts, dangguiwei is 12 to 18 parts, and Gentiana macrophylla is 15 to 25 parts. The mongolian medicine which is made by adopting the raw ingredient medicines has the efficacies of detumescence as well as blood stasis removing, regeneration as well as hemostasis, and heat clearing as well as acesodyne; a large number of experiments and clinical application prove that the mongolian medicine has unique therapeutic effect on various types of the hemorrhoids and has the advantages that the course of treatment is short, the cost is low, no toxic and side effects exist, etc., and the application prospect is very considerable.

The invention provides a method for extracting sophoricoside from sophora fruits, which is characterized by enabling sophora fruit medicinal materials to be smashed, adding 8-12 times amount of alkalinity thin alcohol liquid containing sodium borate to heat and reflux for extracting 2-3 times, adjusting extracting solution to be neutral, passing macroporous resin column, eluting by using ethanol water, enabling eluent to be concentrated to extract, adding defined amount ethyl acetate for dissolving, filtering, dissolving undissolved substance by using acetone, filtering, obtaining filter cakes to conduct backflow dissolving by using 70-90% ethanol, and crystallizing 2-4 times to obtain products. The products manufactured by the method are high in product contents, good in repeatability and large in handling capacity of macroporous resin and have industrialization significances.

The invention relates to a drug for internal hemorrhoid, external hemorrhoid and mixed hemorrhoid treatment, with therapeutic effect to hemorrhoid bleeding, belonging to the field of Chinese patent medicine, comprising cordate houttuyia, purslane, liquirice, angelica, angelica sinensis, gentiana, astragalus, burnet, sophoricoside, plaster, Chinese rhubarb and Chinese honeylocust (or Chinese locust abnormal fruit), which is characterized that the drug can radically curing internal hemorrhoid, external hemorrhoid and mixed hermorrhoid; and the therapeutic effect greatly exceeds prior hemorrhoids drugs on the market according to a research on Chinese herbal medicine market for over thirty years. The drug has the advantages of world advanced radical therapeutic effect for hemorrhoids, safety and reliability, adequacy of drug resource, low cost and simple manufacturing technique, hopefully to be recognized soon.

The invention relates to application of sophoricoside in the preparation of a drug for preventing and / or remedying postmenopausal female articular cartilage degeneration or osteoarthritis. The sophoricoside has obvious curative effect, does not have side effects on uterus, breast and the like caused by animal estrogen, is convenient for extracting and has abundant sources and wide market prospect.

The invention provides a medicine preparation containing sanguisorba and sophoricoside, which comprises medicine components and medicine auxiliary materials. The medicine components for each 500g of the medicine preparation comprises the following materials in weight portion: 104g of sanguisorba, 157g of sophoricoside, 104g of sophora flower, 52g of rhubarb, 104g of scutellaria, 104g of rehmanniae, 52g of angelica, 52g of red peony root, 13g of safflower, 52g of radix sileris, 52g of schizonepeta spike and 52g of bitter orange. The medicine preparation keeps the fixed mixing ratio of the material components in the original formula, has the same curative effect and is easier to use widely.

The invention discloses a quality evaluation method of sophora japonica-scutellaria baicalensis ointment. The method is characterized in that a method of combining fingerprint spectrum similarity analysis and index component relative content analysis is adopted; when the similarity between the fingerprint spectrum of the to-be-detected sophora japonica-scutellaria baicalensis ointment and the contrast fingerprint spectrum of the sophora japonica-scutellaria baicalensis ointment is higher than 0.95, index component relative content analysis is carried out, and an HPLC-DAD method is adopted forfingerprint spectrum similarity analysis and index component relative content analysis of the sophora japonica-scutellaria baicalensis ointment. The method is verified by methods such as precision, stability, repeatability and the like, is accurate and sensitive, compares whether 21 common peaks exist in the fingerprint spectrum or not, has a large amount of information, can effectively evaluate the quality of the sophora japonica-scutellaria baicalensis ointment, and can realize qualitative analysis. The sophoricoside, the paeoniflorin and the baicalin are adopted to realize synchronous monitoring of 21 components, so that the method is more efficient and convenient; particularly, the relative content control of 21 index components of the sophora japonica-scutellaria baicalensis ointmentcan be realized under the condition of lack of reference substances; and the cost of multi-component content determination can be effectively reduced.

The invention discloses a method for producing long-chain glycosylated sophoricoside, and belongs to the technical field of enzyme engineering and fermentation engineering. The invention provides a method for producing long-chain glycosylated sophoricoside, wherein cyclodextrin glucosyltransferase mutants S77N, Y195S, Y195I, S77N, S77N / Y195S and S77N / Y195I are utilized to improve the content of the long-chain glycosylated sophoricoside remarkably, and through the optimization of reaction conditions, the content of the long-chain glycosylated sophoricoside in reaction liquid is increased to 9.8g / L, and the ratio of the content of the long-chain glycosylated sophoricoside to the content of the total glycosylated sophoricoside in the reaction solution to 45%.

The invention relates to a rodenticide resisting breeding of female rats by taking sophoricoside as an active ingredient, belonging to the technical fields of biological prevention and control. The damage caused by rats is always a serious disaster problem in the world, which causes damage to agriculture, forestry and animal husbandry, seriously damages ecological environment and threatens human health. Through the years, people use multiple methods to control the population quantity of rats, and a method for controlling the population quantity of the rats by a breeding-resisting technique is a new technique developed in recent years. The invention relates to the rodenticide resisting breeding of female rats by taking sophoricoside as an active ingredient, which is used for controlling the murine reproduction. Compared with a traditional ecological deratization method and chemical poison bait method, the breeding resisting method has long effective time, obvious control effect, small toxic and side effects on the environment and the like, and can more effectively inhibit the super compensatory effect of rat population. If the rodenticide resisting breeding of female rats provided by the invention can be applied to the production of agriculture, forestry and animal husbandry, the rat population can be effectively controlled and the wide economic benefit can be achieved.

The invention relates to an Aspergillus aculeatus ZJ bacterial strain and an application thereof. The collection number of the Aspergillus aculeatus ZJ is CCTCC NO.M2011264. The bacterial strain is mainly used for converting effective components of the pod of Chinese scholartree to produce 5,7,8,4'-tetrahydroxyisoflavone. The specific method provided by the invention comprises the following steps of: acting the Aspergillus aculeatus ZJ with strong specificity or the produced enzyme on the pod of Chinese scholartree or extracting solution thereof; converting glucosides compounds including sophoricoside and the like, genistein and the like to produce the 5,7,8,4'-tetrahydroxyisoflavone. The invention provides the method for producing the 5,7,8,4'-tetrahydroxyisoflavone through utilizing the Aspergillus aculeatus with high specificity and high conversion efficiency to convert the sophoricoside; and the method provided by the invention has moderate conditions, green process and simple requirements on the process and the device, and has a very good application value.

The invention provides a rodenticide for resisting the breeding of the female lasiopodomys brandtii, which adopts sophoricoside as the active ingredient, belonging to the technical field of biological prevention and treatment. The lasiopodomys brandtii mainly inhabits on the meadow with vegetation degradation, the population is strong in fertility, the quantitive variation amplitude each year and each season is great, the breeding is rapid, the collapsing force for the steppe vegetation is strong, therefore, the lasiopodomys brandtii is a formidable enemy for the animal husbandry, and is one of the main rat damages in the Inner Mongolia steppe, and the rat damage severely damages the ecological environment, and also threatens the human health. For a long time, people adopt many methods to control the population quantity of the lasiopodomys brandtii, wherein the method for controlling the population quantity of the injurious rats by adopting the antifertility technology is a novel technology developed in recent years. The invention relates to the female sterile rodenticide prepared by taking sophoricoside as the active ingredient, which is used for controlling the breeding of the lasiopodomys brandtii. Compared with the traditional ecological deratting method and chemical poison bait method, the antifertility technology has the advantages of long acting time, obvious control effect, small toxic and side effects caused to the environment, and the like, and can more effectively inhibit the over-compensatory effect of the population of the injurious rats. If the novel technology is applied to the agriculture-forestry-stockbreeding production, the population quantity of the injurious rats can be effectively controlled, and thus the broad economic benefit is obtained.

The invention relates to an extraction method of genistein. The method comprises the following steps: fully mixing the grinded raw material pagodatree pod with the grain size of 20-80 meshes and 80-95v% ethanol in the ratio of 1 / 8-1 / 15(w / v), pumping into a hydrodynamic force sonic energy generator, introducing sophoricoside to extraction solvent under the action of acoustic wave with the frequency of 1k-3kHz and the sound pressure level of 216-236dB which is generated by the flowing of the hydrodynamic force sonic energy generator, while performing further hydrolysis; after the solid-liquid separation, using ethanol to dissolve water-insoluble precipitate which is obtained by removing solvent in liquid part, after the ethanol dissolving process, performing silica gel column chromatography, using ethanol for elution, collecting the eluant, and removing solvent to obtain genistein. The method is a green extraction method without additional energy under the action of hydrodynamic force sound energy, the genistein can be obtained directly, the extraction process can be completed without any additional auxiliary component at room temperature, high purity extract can be obtained, and the damage of the effective ingredient can be avoided mostly. The treatment process is simple, the equipment is stable and reliable, the extraction equipment and the floor space are greatly reduced, andthe continuous operation can be realized, the unit capacity is high, the extraction time is greatly reduced, and the extraction cost is only 1 / 10-1 / 20 of that of the conventional method.

The invention discloses a kind of Rhizopus oryzae LJH3 and its application in the preparation of genistein by biotransforming sophoraside. The application is the fermented liquid obtained by fermenting and cultivating Rhizopus oryzae LJH3, or the fermented liquid is filtered to remove bacteria. The mixture of the filtrate of the body and the equal volume of distilled water is used as a biocatalyst, and the transformation system is formed with sophorin as the substrate. The transformation reaction is carried out at 30-38°C and 200-250r / min constant temperature oscillation. After the transformation reaction is completed, The conversion liquid is separated and purified to obtain genistein; in the present invention, when the substrate feeding concentration is 1 g / L, the conversion yield of genistein is 84.2%, and the purity is 92.5%. The invention converts low-activity sophorin into genistein with better activity, which is an optional production method for producing genistein. The process has the advantages of low production cost, high conversion rate, and small environmental pollution.

The invention discloses a detection method of a fructus-sophorae formula granule. The invention also discloses a detection method of sophoricoside in a fructus-sophorae preparation. According to a quality detection method of the fructus-sophorae formula granule, which is provided by the invention, the thin-layer chromatography of a fructus sophorae containing formula granule and a content determination method of the fructus-sophorae formula granule are established; the detection method is simple; the invention also provides the detection method of the sophoricoside in the fructus-sophorae preparation; and the method is high in detection accuracy and has a favorable application prospect.

The invention discloses a medicine composition for treating depression and a preparation method thereof. The medicine composition is prepared from neotorularia humilis, sophoricoside, abelmoschus manihot seeds and glepidotin C, can be prepared into various dosage forms according to a conventional preparation process and has a remarkable treatment effect on treating depression.

The invention discloses a pharmaceutical composition for treating vitreous opacity and a preparation method thereof. The pharmaceutical composition is prepared from pokeberry root saponin A, common anotis herb, stenosolenium saxatile roots, cucurbitacine L, vernomenin and sophoricoside in a matching mode, various preparations can be prepared according to a conventional preparation process, and the treatment effect on vitreous opacity is remarkable.

The invention belongs to the technical field of medicine research, particularly relates to an experimental method for determining the durability of a pharmacopoeia method by using the content of huaijiao pills, and provides the following detection method for detecting the content of huaijiao pills: bonded phenyl porous silica gel microspheres serve as a filling agent (ChromCoreTM Phenyl 5 [mu]m 4.6*150mm); a 2% glacial acetic acid solution is used as a mobile phase A, a methanol-acetonitrile (2:1) mixed solution is used as a mobile phase B, and the flow velocity is 1.2 ml per minute; the detection wavelength of sophoricoside is 260 nm, and the detection wavelength of naringin and baicalin is 280 nm, and the column temperature is 50 DEG C. According to the present invention, the test on the huaijiao pill content detection method in the 2015 Chinese Pharmacopoeia finds that the result difference is large when the content of the same sample is determined by using the phenyl columns of different brands, the method capable of accurately detecting the huaijiao pill content data is provided according to the experiment result, and the durability is strong, and the method is worthy of vigorous promotion.

The invention relates to a drug for internal hemorrhoid, external hemorrhoid and mixed hemorrhoid treatment, with therapeutic effect to hemorrhoid bleeding, belonging to the field of Chinese patent medicine, comprising cordate houttuyia, purslane, liquirice, angelica, angelica sinensis, gentiana, astragalus, burnet, sophoricoside, plaster, Chinese rhubarb and Chinese honeylocust (or Chinese locust abnormal fruit), which is characterized that the drug can radically curing internal hemorrhoid, external hemorrhoid and mixed hermorrhoid; and the therapeutic effect greatly exceeds prior hemorrhoids drugs on the market according to a research on Chinese herbal medicine market for over thirty years. The drug has the advantages of world advanced radical therapeutic effect for hemorrhoids, safety and reliability, adequacy of drug resource, low cost and simple manufacturing technique, hopefully to be recognized soon.

The invention relates to a process for preparing genistein. The process is characterized by: directly using an inorganic solvent to extract dye wood glucoside (sophoricoside), and performing acid hydrolysis after precipitation and purification to generate genistein or using the inorganic solvent to extract a sophoricoside mixture, then directly performing acid hydrolysis to generate genistein, andpurifying. Two improving processes are characterized in that steps of alcohol extraction and degreasing through organic solvents like trichloromethane (chloroform), ethyl ether and ethyl acetate areomitted, so that environment protection is facilitated, the steps are simplified, cost is saved, yield is increased, and the process is suitable for mass production. The genistein prepared by the process can be applied in preventing and treating osteoporosis, female climacteric syndrome, tumor and cardiovascular and cerebrovascular disease and resisting radiation and belongs to the field of drum preparation and drug treatment.