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Novel technology for extracting genistein

A technology of genistein and a new process, applied in the direction of organic chemistry, etc., can solve the problems of difficult industrial production, organic solvent residue, high production cost, etc., and achieve the goal of increasing product yield and content, low cost, and increasing yield Effect

Inactive Publication Date: 2012-02-15
李荣立
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the previous process has high production costs, low product yield, and residual organic solvents, making it difficult to adapt to the requirements of industrial production.

Method used

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  • Novel technology for extracting genistein

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Experimental program
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Effect test

Embodiment 1

[0025] Sophora japonica was crushed into 30 meshes, weighed 10kg, added 80kg of water, heated and refluxed for 3 times, 4 hours each time, combined decoction, concentrated until the specific gravity of the extract was 1.16. Add 245 kg of 2% dilute sulfuric acid, heat to reflux for 0.5 hours, and hydrolyze at 70°C. After cooling, filter, wash the filter cake with water to remove acid, and obtain a black crude product. Add 20 times the amount (mass ratio) of methanol to the crude product for heating and dissolving, cool and crystallize, and then change to 20 times the amount (mass ratio) of absolute ethanol for heating and dissolving after repeated crystallization, cool and crystallize, then vacuum dry to obtain genistein crystals 160 grams, the yield is 1.6%, and the purity of genistein is greater than 98%.

Embodiment 2

[0027] Sophora japonica was crushed into 60 mesh, weighed 10kg, added 90kg of water, heated and refluxed for 3 times, 4 hours each time, combined decoction, concentrated until the specific gravity of the extract was 1.18. Add 245 kg of 2% dilute sulfuric acid, heat to reflux for 0.5 hours, and hydrolyze at 80°C. After cooling, filter, wash the filter cake with water to remove acid, and obtain a black crude product. Add 30 times the amount (mass ratio) of methanol to the crude product for heating and dissolving, cool and crystallize, and then use 30 times the amount (mass ratio) of absolute ethanol to heat and dissolve after repeated crystallization, cool and crystallize, then vacuum dry to obtain genistein crystals 183 grams, the yield is 1.83%, and the purity of genistein is greater than 98%.

Embodiment 3

[0029] Sophora japonica was crushed into 30 meshes, weighed 10kg, added 100kg of water, heated and refluxed for 3 times, 4 hours each time, combined decoction, and concentrated until the specific gravity of the extract was 1.16. Add 245 kg of 2% dilute hydrochloric acid, heat to reflux for 0.5 hours, and hydrolyze at 90°C. After cooling, filter, wash the filter cake with water to remove acid, and obtain a black crude product. Add 30 times the amount (mass ratio) of methanol to the crude product for heating and dissolving, cool and crystallize, and then use 30 times the amount (mass ratio) of absolute ethanol to heat and dissolve after repeated crystallization, cool and crystallize, then vacuum dry to obtain genistein crystals 195 grams, the yield is 1.95%, and the purity of genistein is greater than 98%.

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Abstract

The invention discloses a novel technology for extracting genistein by using sophoricoside as a raw material, comprising the following steps of: crushing sophoricoside, heating by water and carrying out reflux extraction; concentrating a water decoction to an extract, followed by reflux and hydrolysis; cooling and filtering to obtain a crude product; carrying out recrystallization on the crude product to obtain the genistein with the purity being over 98%. The invention has the following advantages: the price of raw materials is low, the product output and high purity are high, the production cost is low, the technology is simple and easy to operate, the requirement on equipment is low and reagents are easy to recover.

Description

technical field [0001] The present invention relates to a new process for extracting genistein, especially a process for extracting and purifying genistein from leguminous plant Sophora japonica, and the role of genistein in preventing and treating osteoporosis, women Application of menopausal syndrome, tumor and cardiovascular and cerebrovascular diseases. It belongs to the field of medicine. Background technique [0002] Genistein is an isoflavone compound derived from legumes and dentate plants, and its chemical name is 4',5,7-trihydroxyisoflavone. It is the main active ingredient of soybean isoflavones, which has estrogen-like effects in the metabolism in the body, and is called phytoestrogens. Genistein has various pharmacological effects and biological activities such as preventing early atherosclerosis and chronic vascular diseases, tumors, and women's menopausal syndrome. Genistein is a powerful inhibitor of tyrosine protein kinase and topoisomerase, through the r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36
Inventor 李荣立赵素芬
Owner 李荣立
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