Method for extracting flavones ingredient and kaempferol from sophora fruit
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A technology of flavonoids and kaempferol, which is applied in the direction of organic chemistry, can solve the problems that kaempferol is not mentioned, and the flavonoid component kaempferol has not been extracted, so as to achieve the effect of high comprehensive utilization, low cost and good product quality
Inactive Publication Date: 2009-02-04
GUIZHOU UNIV
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Chinese Patent No. ZL03156282.5 "Effective Fractions, Production Methods and Applications of Sophora Flavonoids" discloses isoflavone extracts, flavonol extracts and total flavonoids extracts of Sophora japonicus, and also relates to the industrial production method of the effective fractions and its application in Applications in pharmaceuticals, functional foods or skin care products, and no mention of kaempferol
At present, there is no patent application for the technical scheme of extracting flavonoids from Huaijiao and extracting kaempferol
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Embodiment 1
[0016] Take 90kg of Sophora japonica and add it to the extraction tank, add 710L of tap water, heat to 50°C, extract at a constant temperature for 3 hours, filter the extract with diatomaceous earth until it is clear, pass through the AB-8 macroporous resin column to separate, use pure water, ethanol gradient After elution, the eluate was concentrated and dried to obtain 7.2 kg of Sophora japonicus flavonoids extract, and the total flavonoids purity was ≥50%.
[0017] After that, take 1 kg of the above-mentioned Sophora japonicus extract, add 10 L of hydrolyzate, heat to 60° C., and hydrolyze at constant temperature for 4 hours.
[0018] Finally, adjust the pH to 6-8, evaporate the alcohol, filter, and rinse the filter residue with water for 5 times; after drying the filter residue, extract it with a mixed solvent of ether and methanol, collect the extract, concentrate and dry it to obtain 70g of high-purity kaempferol, kaempferia Vegetarian purity ≥ 90%.
Embodiment 2
[0020] Take 90kg of Sophora japonica and put it into the extraction tank, add 800L of tap water, heat to 80°C, extract at constant temperature for 5 hours, filter the extract with diatomaceous earth until it is clear, separate it through a DM130 macroporous resin column, and elute with pure water and ethanol gradient , concentrating and drying the eluate to obtain 7.6 kg of Sophora flavonoids extract with a purity of ≥51%.
[0021] Afterwards, take 1 kg of the above-mentioned Sophora japonicus extract, add 9 L of hydrolyzate, heat to 55° C., and hydrolyze at constant temperature for 5 hours.
[0022] Finally, adjust the pH to 6-8, distill off the alcohol, filter, rinse the filter residue with water for 6 times, dry the filter residue, extract with a mixed solvent of ether and methanol, collect the extract, concentrate and dry to obtain 72g of high-purity kaempferol. ≥92%.
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Abstract
The invention discloses a method for extracting flavone ingredients and extracting kaempferide from sophoricoside and relates to the medicine. The method comprises the following steps: de-ionized water is added into the raw material of the sophoricoside, and reflux extracting is carried out in heating condition; extracting liquid-purpose diatomite and filtering is carried out; the filter solution is put on a macroporous resin column, impurity is washed by water and the filter solution is eluted by high-concentration ethanol; concentrating and drying is carried out on the eluting solution, thus obtaining the sophoricoside flavone extract; hydrolysate is added into the sophoricoside flavone extract and hydrolyzing is carried out in heating condition; pH value is adjusted to neutral after hydrolysis; steaming and removing alcohol, filtering, washing repeatedly by water are carried out on filter residue; drying and extracting are carried out on the filter residue by an extracting agent of mixed solution of aether and ethanol, the extract solution is steamed and the high-purity kaempferide is obtained. The method can be used for producing high-purity kaempferide flavone extract and kaempferide and has the advantages of simple process, good product quality, high degree of comprehensive utilization, being suitable for industrial production and low cost.
Description
technical field [0001] The invention is related to medicine, and relates to a method for jointly extracting active ingredients from Chinese medicinal materials, in particular to a method for extracting flavonoid components from Sophora japonica and extracting kaempferol. Background technique [0002] Sophora japonica L., a traditional Chinese medicinal material, is the dried fruit of the leguminous plant Sophora japonica L. It is used as medicine because it contains a large amount of flavonoids and isoflavones. It is cold in nature and bitter in taste. , anti-tumor and positive inotropic function to the heart. In recent years, people have been studying the extraction method of its active ingredients, but there are few reports on the extraction method of Kaempferol. Chinese Patent No. ZL03156282.5 "Effective Parts, Production Methods and Applications of Sophora Flavonoids" discloses isoflavone extracts, flavonol extracts and total flavonoids extracts of Sophora japonicus, an...
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